File Download
There are no files associated with this item.
Links for fulltext
(May Require Subscription)
- Publisher Website: 10.1016/j.jneumeth.2007.10.018
- Scopus: eid_2-s2.0-38749109692
- PMID: 18079000
- WOS: WOS:000253824400020
- Find via
Supplementary
- Citations:
- Appears in Collections:
Article: In vivo imaging of murine retinal ganglion cells
Title | In vivo imaging of murine retinal ganglion cells |
---|---|
Authors | |
Keywords | Confocal scanning laser ophthalmoscopy Transgenic mice CFP Retinal ganglion cell imaging Thy-1 |
Issue Date | 2008 |
Citation | Journal of Neuroscience Methods, 2008, v. 168, n. 2, p. 475-478 How to Cite? |
Abstract | Current methods for in vivo retinal ganglion cells (RGCs) imaging involve either retrograde or intravitreal injection of chemical or biological tracers, which are invasive and may require repeated injection for serial long-term assessment. We have developed a confocal scanning laser ophthalmoscope technique (blue-light CSLO or bCSLO) to image retinal ganglion cells (RGCs) in mice expressing cyan fluorescent protein under the control of a Thy-1 promoter. Fluorescent spots corresponding to CFP-expressing retinal ganglion cells were discernable with the bCSLO. 96.1 ± 2.6% of CFP expressing cells also were retrograde labeled with DiI indicating the bCSLO imaged fluorescent spots are RGCs. The imaging of Thy-1 promoter-driven CFP expression in these mice could serve as a sensitive indicator to reflect the integrity of RGCs, and provides a non-invasive method for longitudinal study of the mechanism of RGC degeneration and the effect of neuroprotective agents. © 2007 Elsevier B.V. All rights reserved. |
Persistent Identifier | http://hdl.handle.net/10722/298414 |
ISSN | 2023 Impact Factor: 2.7 2023 SCImago Journal Rankings: 0.935 |
ISI Accession Number ID |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Leung, Christopher K.S. | - |
dc.contributor.author | Lindsey, James D. | - |
dc.contributor.author | Crowston, Jonathan G. | - |
dc.contributor.author | Ju, Won Kyu | - |
dc.contributor.author | Liu, Qwan | - |
dc.contributor.author | Bartsch, Dirk Uwe | - |
dc.contributor.author | Weinreb, Robert N. | - |
dc.date.accessioned | 2021-04-08T03:08:22Z | - |
dc.date.available | 2021-04-08T03:08:22Z | - |
dc.date.issued | 2008 | - |
dc.identifier.citation | Journal of Neuroscience Methods, 2008, v. 168, n. 2, p. 475-478 | - |
dc.identifier.issn | 0165-0270 | - |
dc.identifier.uri | http://hdl.handle.net/10722/298414 | - |
dc.description.abstract | Current methods for in vivo retinal ganglion cells (RGCs) imaging involve either retrograde or intravitreal injection of chemical or biological tracers, which are invasive and may require repeated injection for serial long-term assessment. We have developed a confocal scanning laser ophthalmoscope technique (blue-light CSLO or bCSLO) to image retinal ganglion cells (RGCs) in mice expressing cyan fluorescent protein under the control of a Thy-1 promoter. Fluorescent spots corresponding to CFP-expressing retinal ganglion cells were discernable with the bCSLO. 96.1 ± 2.6% of CFP expressing cells also were retrograde labeled with DiI indicating the bCSLO imaged fluorescent spots are RGCs. The imaging of Thy-1 promoter-driven CFP expression in these mice could serve as a sensitive indicator to reflect the integrity of RGCs, and provides a non-invasive method for longitudinal study of the mechanism of RGC degeneration and the effect of neuroprotective agents. © 2007 Elsevier B.V. All rights reserved. | - |
dc.language | eng | - |
dc.relation.ispartof | Journal of Neuroscience Methods | - |
dc.subject | Confocal scanning laser ophthalmoscopy | - |
dc.subject | Transgenic mice | - |
dc.subject | CFP | - |
dc.subject | Retinal ganglion cell imaging | - |
dc.subject | Thy-1 | - |
dc.title | In vivo imaging of murine retinal ganglion cells | - |
dc.type | Article | - |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1016/j.jneumeth.2007.10.018 | - |
dc.identifier.pmid | 18079000 | - |
dc.identifier.scopus | eid_2-s2.0-38749109692 | - |
dc.identifier.volume | 168 | - |
dc.identifier.issue | 2 | - |
dc.identifier.spage | 475 | - |
dc.identifier.epage | 478 | - |
dc.identifier.isi | WOS:000253824400020 | - |
dc.identifier.issnl | 0165-0270 | - |