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Article: Human Intestinal Organoids Recapitulate Enteric Infections of Enterovirus and Coronavirus

TitleHuman Intestinal Organoids Recapitulate Enteric Infections of Enterovirus and Coronavirus
Authors
KeywordsIntestinal organoids
EV-A71
CVA16
SARS-CoV
SARS-CoV-2
Pathogenicity
Issue Date2021
Citation
Stem Cell Reports, 2021, v. 16, n. 3, p. 493-504 How to Cite?
AbstractEnteroviruses, such as EV-A71 and CVA16, mainly infect the human gastrointestinal tract. Human coronaviruses, including SARS-CoV and SARS-CoV-2, have been variably associated with gastrointestinal symptoms. We aimed to optimize the human intestinal organoids and hypothesize that these optimized intestinal organoids can recapitulate enteric infections of enterovirus and coronavirus. We demonstrate that the optimized human intestinal organoids enable better simulation of the native human intestinal epithelium, and that they are significantly more susceptible to EV-A71 than CVA16. Higher replication of EV-A71 than CVA16 in the intestinal organoids triggers a more vigorous cellular response. However, SARS-CoV and SARS-CoV-2 exhibit distinct dynamics of virus-host interaction; more robust propagation of SARS-CoV triggers minimal cellular response, whereas, SARS-CoV-2 exhibits lower replication capacity but elicits a moderate cellular response. Taken together, the disparate profile of the virus-host interaction of enteroviruses and coronaviruses in human intestinal organoids may unravel the cellular basis of the distinct pathogenicity of these viral pathogens.
Persistent Identifierhttp://hdl.handle.net/10722/297317
ISSN
2023 Impact Factor: 5.9
2023 SCImago Journal Rankings: 2.518
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorZhao, Xiaoyu-
dc.contributor.authorLi, Cun-
dc.contributor.authorLiu, Xiaojuan-
dc.contributor.authorChiu, Man Chun-
dc.contributor.authorWang, Dong-
dc.contributor.authorWei, Yuxuan-
dc.contributor.authorChu, Hin-
dc.contributor.authorCai, Jian Piao-
dc.contributor.authorChan, Ivy Hau-Yee-
dc.contributor.authorWong, Kenneth Kak-Yuen-
dc.contributor.authorChan, Jasper Fuk-Woo-
dc.contributor.authorTo, Kelvin Kai-Wang-
dc.contributor.authorYuen, Kwok Yung-
dc.contributor.authorZhou, Jie-
dc.date.accessioned2021-03-15T07:33:30Z-
dc.date.available2021-03-15T07:33:30Z-
dc.date.issued2021-
dc.identifier.citationStem Cell Reports, 2021, v. 16, n. 3, p. 493-504-
dc.identifier.issn2213-6711-
dc.identifier.urihttp://hdl.handle.net/10722/297317-
dc.description.abstractEnteroviruses, such as EV-A71 and CVA16, mainly infect the human gastrointestinal tract. Human coronaviruses, including SARS-CoV and SARS-CoV-2, have been variably associated with gastrointestinal symptoms. We aimed to optimize the human intestinal organoids and hypothesize that these optimized intestinal organoids can recapitulate enteric infections of enterovirus and coronavirus. We demonstrate that the optimized human intestinal organoids enable better simulation of the native human intestinal epithelium, and that they are significantly more susceptible to EV-A71 than CVA16. Higher replication of EV-A71 than CVA16 in the intestinal organoids triggers a more vigorous cellular response. However, SARS-CoV and SARS-CoV-2 exhibit distinct dynamics of virus-host interaction; more robust propagation of SARS-CoV triggers minimal cellular response, whereas, SARS-CoV-2 exhibits lower replication capacity but elicits a moderate cellular response. Taken together, the disparate profile of the virus-host interaction of enteroviruses and coronaviruses in human intestinal organoids may unravel the cellular basis of the distinct pathogenicity of these viral pathogens.-
dc.languageeng-
dc.relation.ispartofStem Cell Reports-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.subjectIntestinal organoids-
dc.subjectEV-A71-
dc.subjectCVA16-
dc.subjectSARS-CoV-
dc.subjectSARS-CoV-2-
dc.subjectPathogenicity-
dc.titleHuman Intestinal Organoids Recapitulate Enteric Infections of Enterovirus and Coronavirus-
dc.typeArticle-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1016/j.stemcr.2021.02.009-
dc.identifier.pmid33626333-
dc.identifier.scopuseid_2-s2.0-85101326211-
dc.identifier.hkuros326096-
dc.identifier.volume16-
dc.identifier.issue3-
dc.identifier.spage493-
dc.identifier.epage504-
dc.identifier.isiWOS:000631902800011-
dc.identifier.issnl2213-6711-

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