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Article: Proteomic Analysis of Caspofungin-Induced Responses in Planktonic Cells and Biofilms of Candida albicans

TitleProteomic Analysis of Caspofungin-Induced Responses in Planktonic Cells and Biofilms of Candida albicans
Authors
KeywordsCandida albicans
caspofungin
biofilms
antifungal resistance
proteomics
Issue Date2021
PublisherFrontiers Research Foundation. The Journal's web site is located at http://www.frontiersin.org/microbiology/
Citation
Frontiers in Microbiology, 2021, v. 12, p. article no. 639123 How to Cite?
AbstractCandida albicans biofilms display markedly increased antifungal resistance, and the underlying mechanisms remain unclear. This study investigated the signature profiles of C. albicans planktonic cells and biofilms in response to caspofungin (CAS) by mass spectrometry-based shotgun proteomics. We found that C. albicans biofilms were twofold more resistant to CAS with reference to planktonic cells. Notably, 9.6% of C. albicans biofilm cells survived the lethal treatment of CAS (128 mu g/ml), confirmed by LIVE/DEAD staining, confocal laser scanning microscopy (CLSM) and scanning electron microscopy analyses. The responses of C. albicans planktonic cells and biofilms to CAS treatment at respective minimum inhibitory concentrations (MICs) were assessed by high-throughput proteomics and bioinformatics approaches. There were 148 and 224 proteins with >twofold difference identified from the planktonic cells and biofilms, respectively. CAS treatment downregulated several cell wall- and oxidative stress-related proteins. Whereas, CAS-induced action was compensated by markedly increased expression of many other proteins involved in cell wall integrity and stress response (e.g., heat shock proteins). Moreover, considerable expression changes were identified in metabolism-associated proteins like glycolysis, tricarboxylic acid (TCA) cycle and ATP biosynthesis. Importantly, various key proteins for cell wall integrity, stress response and metabolic regulation (e.g., PIL1, LSP1, HSP90, ICL1, and MLS1) were exclusively enriched and implicated in C. albicans biofilms. This study demonstrates that C. albicans biofilms undergo highly complicated yet complex regulation of multiple cellular pathways in response to CAS. Signature proteins essential for modulating cell wall integrity, stress response and metabolic activities may account for the antifungal resistance of C. albicans biofilms.
Persistent Identifierhttp://hdl.handle.net/10722/297281
ISSN
2020 Impact Factor: 5.64
2015 SCImago Journal Rankings: 1.970
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorLi, P-
dc.contributor.authorSeneviratne, CJ-
dc.contributor.authorLuan, Q-
dc.contributor.authorJin, L-
dc.date.accessioned2021-03-08T07:16:44Z-
dc.date.available2021-03-08T07:16:44Z-
dc.date.issued2021-
dc.identifier.citationFrontiers in Microbiology, 2021, v. 12, p. article no. 639123-
dc.identifier.issn1664-302X-
dc.identifier.urihttp://hdl.handle.net/10722/297281-
dc.description.abstractCandida albicans biofilms display markedly increased antifungal resistance, and the underlying mechanisms remain unclear. This study investigated the signature profiles of C. albicans planktonic cells and biofilms in response to caspofungin (CAS) by mass spectrometry-based shotgun proteomics. We found that C. albicans biofilms were twofold more resistant to CAS with reference to planktonic cells. Notably, 9.6% of C. albicans biofilm cells survived the lethal treatment of CAS (128 mu g/ml), confirmed by LIVE/DEAD staining, confocal laser scanning microscopy (CLSM) and scanning electron microscopy analyses. The responses of C. albicans planktonic cells and biofilms to CAS treatment at respective minimum inhibitory concentrations (MICs) were assessed by high-throughput proteomics and bioinformatics approaches. There were 148 and 224 proteins with >twofold difference identified from the planktonic cells and biofilms, respectively. CAS treatment downregulated several cell wall- and oxidative stress-related proteins. Whereas, CAS-induced action was compensated by markedly increased expression of many other proteins involved in cell wall integrity and stress response (e.g., heat shock proteins). Moreover, considerable expression changes were identified in metabolism-associated proteins like glycolysis, tricarboxylic acid (TCA) cycle and ATP biosynthesis. Importantly, various key proteins for cell wall integrity, stress response and metabolic regulation (e.g., PIL1, LSP1, HSP90, ICL1, and MLS1) were exclusively enriched and implicated in C. albicans biofilms. This study demonstrates that C. albicans biofilms undergo highly complicated yet complex regulation of multiple cellular pathways in response to CAS. Signature proteins essential for modulating cell wall integrity, stress response and metabolic activities may account for the antifungal resistance of C. albicans biofilms.-
dc.languageeng-
dc.publisherFrontiers Research Foundation. The Journal's web site is located at http://www.frontiersin.org/microbiology/-
dc.relation.ispartofFrontiers in Microbiology-
dc.rightsThis Document is Protected by copyright and was first published by Frontiers. All rights reserved. It is reproduced with permission.-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.subjectCandida albicans-
dc.subjectcaspofungin-
dc.subjectbiofilms-
dc.subjectantifungal resistance-
dc.subjectproteomics-
dc.titleProteomic Analysis of Caspofungin-Induced Responses in Planktonic Cells and Biofilms of Candida albicans-
dc.typeArticle-
dc.identifier.emailJin, L: ljjin@hkucc.hku.hk-
dc.identifier.authorityJin, L=rp00028-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.3389/fmicb.2021.639123-
dc.identifier.pmid33679674-
dc.identifier.pmcidPMC7931687-
dc.identifier.hkuros321495-
dc.identifier.volume12-
dc.identifier.spagearticle no. 639123-
dc.identifier.epagearticle no. 639123-
dc.identifier.isiWOS:000625236800001-
dc.publisher.placeSwitzerland-

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