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- Publisher Website: 10.1002/1873-3468.13923
- Scopus: eid_2-s2.0-85091172874
- PMID: 32888212
- WOS: WOS:000570297800001
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Article: Crystal structure of the rice acyl‐CoA‐binding protein OsACBP2 in complex with C18:3‐CoA reveals a novel pattern of binding to acyl‐CoA esters
Title | Crystal structure of the rice acyl‐CoA‐binding protein OsACBP2 in complex with C18:3‐CoA reveals a novel pattern of binding to acyl‐CoA esters |
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Authors | |
Keywords | acyl-CoA binding multiangle light scattering Oryza sativa protein–lipid interaction X-ray diffraction |
Issue Date | 2020 |
Publisher | Elsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/febslet |
Citation | FEBS Letters, 2020, v. 594 n. 21, p. 3568-3575 How to Cite? |
Abstract | Acyl-CoA-binding proteins (ACBPs) are a family of proteins that bind acyl-CoA esters at a conserved acyl-CoA-binding domain. ACBPs maintain intracellular acyl-CoA pools to regulate lipid metabolism. Here, we report on the structure of rice OsACBP2 in complex with C18:3-CoA ester. The residues Y33, K34 and K56 of OsACBP2 play a crucial role in binding the CoA group, while residues N23, L27, K52 and Y55 in one molecule of OsACBP2 cooperate with L27, L28, A59 and A62 from another anchoring the fatty acyl group. Multiangle light scattering assays indicate that OsACBP2 binds C18:3-CoA as a monomer. The first complex structure of a plant ACBP binding with C18:3-CoA is therefore presented, providing a novel model for the interaction between an acyl-CoA ester and the acyl-CoA-binding domain(s). |
Persistent Identifier | http://hdl.handle.net/10722/295891 |
ISSN | 2023 Impact Factor: 3.0 2023 SCImago Journal Rankings: 1.208 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Jin, J | - |
dc.contributor.author | Guo, ZH | - |
dc.contributor.author | Hao, Q | - |
dc.contributor.author | Chye, ML | - |
dc.date.accessioned | 2021-02-08T08:15:30Z | - |
dc.date.available | 2021-02-08T08:15:30Z | - |
dc.date.issued | 2020 | - |
dc.identifier.citation | FEBS Letters, 2020, v. 594 n. 21, p. 3568-3575 | - |
dc.identifier.issn | 0014-5793 | - |
dc.identifier.uri | http://hdl.handle.net/10722/295891 | - |
dc.description.abstract | Acyl-CoA-binding proteins (ACBPs) are a family of proteins that bind acyl-CoA esters at a conserved acyl-CoA-binding domain. ACBPs maintain intracellular acyl-CoA pools to regulate lipid metabolism. Here, we report on the structure of rice OsACBP2 in complex with C18:3-CoA ester. The residues Y33, K34 and K56 of OsACBP2 play a crucial role in binding the CoA group, while residues N23, L27, K52 and Y55 in one molecule of OsACBP2 cooperate with L27, L28, A59 and A62 from another anchoring the fatty acyl group. Multiangle light scattering assays indicate that OsACBP2 binds C18:3-CoA as a monomer. The first complex structure of a plant ACBP binding with C18:3-CoA is therefore presented, providing a novel model for the interaction between an acyl-CoA ester and the acyl-CoA-binding domain(s). | - |
dc.language | eng | - |
dc.publisher | Elsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/febslet | - |
dc.relation.ispartof | FEBS Letters | - |
dc.subject | acyl-CoA binding | - |
dc.subject | multiangle light scattering | - |
dc.subject | Oryza sativa | - |
dc.subject | protein–lipid interaction | - |
dc.subject | X-ray diffraction | - |
dc.title | Crystal structure of the rice acyl‐CoA‐binding protein OsACBP2 in complex with C18:3‐CoA reveals a novel pattern of binding to acyl‐CoA esters | - |
dc.type | Article | - |
dc.identifier.email | Jin, J: jinjing@hku.hk | - |
dc.identifier.email | Guo, ZH: zhguo1990@hku.hk | - |
dc.identifier.email | Hao, Q: qhao@hku.hk | - |
dc.identifier.email | Chye, ML: mlchye@hku.hk | - |
dc.identifier.authority | Hao, Q=rp01332 | - |
dc.identifier.authority | Chye, ML=rp00687 | - |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1002/1873-3468.13923 | - |
dc.identifier.pmid | 32888212 | - |
dc.identifier.scopus | eid_2-s2.0-85091172874 | - |
dc.identifier.hkuros | 321231 | - |
dc.identifier.hkuros | 322519 | - |
dc.identifier.volume | 594 | - |
dc.identifier.issue | 21 | - |
dc.identifier.spage | 3568 | - |
dc.identifier.epage | 3575 | - |
dc.identifier.isi | WOS:000570297800001 | - |
dc.publisher.place | Netherlands | - |