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Article: Impact of unilateral ureteral obstruction on cognition and neurodegeneration

TitleImpact of unilateral ureteral obstruction on cognition and neurodegeneration
Authors
KeywordsRenal impairment
Cognitive impairment
Synapse
Tau phosphorylation
Inflammation
Issue Date2021
PublisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/brainresbull
Citation
Brain Research Bulletin, 2021, v. 169, p. 112-127 How to Cite?
AbstractIntroduction: Cognitive impairment is a common complication in chronic kidney disease (CKD) patients. Currently, limited types of animal models are available for studying cognitive impairment in CKD. We used unilateral ureteral obstruction (UUO) in mice as an animal model to study the cognitive changes and related pathology under prolonged renal impairment Methods: UUO was performed in 8-week-old male C57BL/6 N mice with double-ligation of their left ureter. A sham group was subjected to the same experimental procedure without ureteral obstruction. Cognitive and behavioral tests were performed to examine potential changes in cognition and behavior at 2, 4 and 12 weeks after surgery. Sera were collected, and kidneys and brains were harvested for the detection of systemic inflammation markers and neurodegenerative changes. Results: These mice displayed weak performance in the novel object recognition test, Y-maze test, and puzzle box test compared to the sham group. Reductions in synaptic proteins such as synapsin-1, synaptophysin, synaptotagmin, PSD95, NMDAR2B and AMPAR were confirmed by western blot analysis. Histological examination revealed elevated levels of Nrf2 and 8-hydroxyguanosine, and hyperphosphorylation of tau in the hippocampus. UUO mice also had increased levels of C-reactive protein (CRP) and TNF-alpha. Conclusions: We characterized the cognitive and neumpathological changes in UUO mice. The results show that this mouse model can be used to further study cognitive changes related to chronic renal impairment.
Persistent Identifierhttp://hdl.handle.net/10722/295834
ISSN
2021 Impact Factor: 3.715
2020 SCImago Journal Rankings: 1.097
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorHo, YS-
dc.contributor.authorLau, CF-
dc.contributor.authorLee, K-
dc.contributor.authorTan, JY-
dc.contributor.authorLee, J-
dc.contributor.authorYung, S-
dc.contributor.authorChang, RCC-
dc.date.accessioned2021-02-08T08:14:40Z-
dc.date.available2021-02-08T08:14:40Z-
dc.date.issued2021-
dc.identifier.citationBrain Research Bulletin, 2021, v. 169, p. 112-127-
dc.identifier.issn0361-9230-
dc.identifier.urihttp://hdl.handle.net/10722/295834-
dc.description.abstractIntroduction: Cognitive impairment is a common complication in chronic kidney disease (CKD) patients. Currently, limited types of animal models are available for studying cognitive impairment in CKD. We used unilateral ureteral obstruction (UUO) in mice as an animal model to study the cognitive changes and related pathology under prolonged renal impairment Methods: UUO was performed in 8-week-old male C57BL/6 N mice with double-ligation of their left ureter. A sham group was subjected to the same experimental procedure without ureteral obstruction. Cognitive and behavioral tests were performed to examine potential changes in cognition and behavior at 2, 4 and 12 weeks after surgery. Sera were collected, and kidneys and brains were harvested for the detection of systemic inflammation markers and neurodegenerative changes. Results: These mice displayed weak performance in the novel object recognition test, Y-maze test, and puzzle box test compared to the sham group. Reductions in synaptic proteins such as synapsin-1, synaptophysin, synaptotagmin, PSD95, NMDAR2B and AMPAR were confirmed by western blot analysis. Histological examination revealed elevated levels of Nrf2 and 8-hydroxyguanosine, and hyperphosphorylation of tau in the hippocampus. UUO mice also had increased levels of C-reactive protein (CRP) and TNF-alpha. Conclusions: We characterized the cognitive and neumpathological changes in UUO mice. The results show that this mouse model can be used to further study cognitive changes related to chronic renal impairment.-
dc.languageeng-
dc.publisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/brainresbull-
dc.relation.ispartofBrain Research Bulletin-
dc.subjectRenal impairment-
dc.subjectCognitive impairment-
dc.subjectSynapse-
dc.subjectTau phosphorylation-
dc.subjectInflammation-
dc.titleImpact of unilateral ureteral obstruction on cognition and neurodegeneration-
dc.typeArticle-
dc.identifier.emailLau, CF: jefferyl@hku.hk-
dc.identifier.emailLee, K: leeackt@hku.hk-
dc.identifier.emailYung, S: ssyyung@hku.hk-
dc.identifier.emailChang, RCC: rccchang@hku.hk-
dc.identifier.authorityYung, S=rp00455-
dc.identifier.authorityChang, RCC=rp00470-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.brainresbull.2021.01.001-
dc.identifier.pmid33422661-
dc.identifier.scopuseid_2-s2.0-85099806912-
dc.identifier.hkuros321209-
dc.identifier.volume169-
dc.identifier.spage112-
dc.identifier.epage127-
dc.identifier.isiWOS:000620839800013-
dc.publisher.placeUnited States-

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