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Article: A DYRK1B-dependent pathway suppresses rDNA transcription in response to DNA damage

TitleA DYRK1B-dependent pathway suppresses rDNA transcription in response to DNA damage
Authors
Issue Date2021
PublisherOxford University Press: Policy C - Creative Commons Attribution and Creative Commons Attribution Non-Commercial. The Journal's web site is located at http://nar.oxfordjournals.org/
Citation
Nucleic Acids Research, 2021, v. 49 n. 3, p. 1485-1496 How to Cite?
AbstractDNA double-strand breaks (DSBs) at ribosomal gene loci trigger inhibition of ribosomal DNA (rDNA) transcription and extensive nucleolar reorganization, including the formation of nucleolar caps where rDNA DSBs engage with canonical DSB signaling and repair factors. While these nucleolar responses underlie maintenance of rDNA stability, the molecular components that drive each of these events remain to be defined. Here we report that full suppression of rRNA synthesis requires the DYRK1B kinase, a nucleolar DSB response that can be uncoupled from ATM-mediated DSB signaling events at the nucleolar periphery. Indeed, by targeting DSBs onto rDNA arrays, we uncovered that chemical inhibition or genetic inactivation of DYRK1B led to sustained nucleolar transcription. Not only does DYRK1B exhibit robust nucleolar accumulation following laser micro-irradiation across cell nuclei, we further showed that DYRK1B is required for rDNA DSB repair and rDNA copy number maintenance, and that DYRK1B-inactivated cells are hypersensitised to DSBs induced at the rDNA arrays. Together, our findings not only identify DYRK1B as a key signaling intermediate that coordinates DSB repair and rDNA transcriptional activities, but also support the idea of specialised DSB responses that operate within the nucleolus to preserve rDNA integrity.
Persistent Identifierhttp://hdl.handle.net/10722/295832
ISSN
2023 Impact Factor: 16.6
2023 SCImago Journal Rankings: 7.048
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorDONG, C-
dc.contributor.authorAN, L-
dc.contributor.authorYu, CH-
dc.contributor.authorHuen, MSY-
dc.date.accessioned2021-02-08T08:14:39Z-
dc.date.available2021-02-08T08:14:39Z-
dc.date.issued2021-
dc.identifier.citationNucleic Acids Research, 2021, v. 49 n. 3, p. 1485-1496-
dc.identifier.issn0305-1048-
dc.identifier.urihttp://hdl.handle.net/10722/295832-
dc.description.abstractDNA double-strand breaks (DSBs) at ribosomal gene loci trigger inhibition of ribosomal DNA (rDNA) transcription and extensive nucleolar reorganization, including the formation of nucleolar caps where rDNA DSBs engage with canonical DSB signaling and repair factors. While these nucleolar responses underlie maintenance of rDNA stability, the molecular components that drive each of these events remain to be defined. Here we report that full suppression of rRNA synthesis requires the DYRK1B kinase, a nucleolar DSB response that can be uncoupled from ATM-mediated DSB signaling events at the nucleolar periphery. Indeed, by targeting DSBs onto rDNA arrays, we uncovered that chemical inhibition or genetic inactivation of DYRK1B led to sustained nucleolar transcription. Not only does DYRK1B exhibit robust nucleolar accumulation following laser micro-irradiation across cell nuclei, we further showed that DYRK1B is required for rDNA DSB repair and rDNA copy number maintenance, and that DYRK1B-inactivated cells are hypersensitised to DSBs induced at the rDNA arrays. Together, our findings not only identify DYRK1B as a key signaling intermediate that coordinates DSB repair and rDNA transcriptional activities, but also support the idea of specialised DSB responses that operate within the nucleolus to preserve rDNA integrity.-
dc.languageeng-
dc.publisherOxford University Press: Policy C - Creative Commons Attribution and Creative Commons Attribution Non-Commercial. The Journal's web site is located at http://nar.oxfordjournals.org/-
dc.relation.ispartofNucleic Acids Research-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.titleA DYRK1B-dependent pathway suppresses rDNA transcription in response to DNA damage-
dc.typeArticle-
dc.identifier.emailYu, CH: chyu1@hku.hk-
dc.identifier.emailHuen, MSY: huen.michael@hku.hk-
dc.identifier.authorityYu, CH=rp01930-
dc.identifier.authorityHuen, MSY=rp01336-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1093/nar/gkaa1290-
dc.identifier.scopuseid_2-s2.0-85102219916-
dc.identifier.hkuros321207-
dc.identifier.volume49-
dc.identifier.issue3-
dc.identifier.spage1485-
dc.identifier.epage1496-
dc.identifier.isiWOS:000626724500024-
dc.publisher.placeUnited Kingdom-

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