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- Publisher Website: 10.1007/978-1-0716-0892-0_1
- Scopus: eid_2-s2.0-85094935016
- PMID: 33125641
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Article: High-Throughput Protein Engineering by Massively Parallel Combinatorial Mutagenesis
Title | High-Throughput Protein Engineering by Massively Parallel Combinatorial Mutagenesis |
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Authors | |
Keywords | CombiSEAL Protein engineering Combinatorial mutagenesis Combinatorial genetics en masse High-throughput screening |
Issue Date | 2021 |
Publisher | Humana Press, Inc. The Journal's web site is located at http://link.springer.com/bookseries/7651 |
Citation | Methods in Molecular Biology, 2021, v. 2199, p. 3-12 How to Cite? |
Abstract | Exploring how combinatorial mutations can be combined to optimize protein functions is important to guide protein engineering. Given the vast combinatorial space of changing multiple amino acids, identifying the top-performing variants from a large number of mutants might not be possible without a high-throughput gene assembly and screening strategy. Here we describe the CombiSEAL platform, a strategy that allows for modularization of any protein sequence into multiple segments for mutagenesis and barcoding, and seamless single-pot ligations of different segments to generate a library of combination mutants linked with concatenated barcodes at one end. By reading the barcodes using next-generation sequencing, activities of each protein variant during the protein selection process can be easily tracked in a high-throughput manner. CombiSEAL not only allows the identification of better protein variants but also enables the systematic analyses to distinguish the beneficial, deleterious, and neutral effects of combining different mutations on protein functions. |
Persistent Identifier | http://hdl.handle.net/10722/295768 |
ISSN | 2023 SCImago Journal Rankings: 0.399 |
DC Field | Value | Language |
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dc.contributor.author | Wan, YK | - |
dc.contributor.author | Choi, GC | - |
dc.contributor.author | Wong, SL | - |
dc.date.accessioned | 2021-02-08T08:13:44Z | - |
dc.date.available | 2021-02-08T08:13:44Z | - |
dc.date.issued | 2021 | - |
dc.identifier.citation | Methods in Molecular Biology, 2021, v. 2199, p. 3-12 | - |
dc.identifier.issn | 1064-3745 | - |
dc.identifier.uri | http://hdl.handle.net/10722/295768 | - |
dc.description.abstract | Exploring how combinatorial mutations can be combined to optimize protein functions is important to guide protein engineering. Given the vast combinatorial space of changing multiple amino acids, identifying the top-performing variants from a large number of mutants might not be possible without a high-throughput gene assembly and screening strategy. Here we describe the CombiSEAL platform, a strategy that allows for modularization of any protein sequence into multiple segments for mutagenesis and barcoding, and seamless single-pot ligations of different segments to generate a library of combination mutants linked with concatenated barcodes at one end. By reading the barcodes using next-generation sequencing, activities of each protein variant during the protein selection process can be easily tracked in a high-throughput manner. CombiSEAL not only allows the identification of better protein variants but also enables the systematic analyses to distinguish the beneficial, deleterious, and neutral effects of combining different mutations on protein functions. | - |
dc.language | eng | - |
dc.publisher | Humana Press, Inc. The Journal's web site is located at http://link.springer.com/bookseries/7651 | - |
dc.relation.ispartof | Methods in Molecular Biology | - |
dc.rights | The original publication is available at www.springerlink.com | - |
dc.subject | CombiSEAL | - |
dc.subject | Protein engineering | - |
dc.subject | Combinatorial mutagenesis | - |
dc.subject | Combinatorial genetics en masse | - |
dc.subject | High-throughput screening | - |
dc.title | High-Throughput Protein Engineering by Massively Parallel Combinatorial Mutagenesis | - |
dc.type | Article | - |
dc.identifier.email | Choi, GC: gigichoi@hku.hk | - |
dc.identifier.email | Wong, SL: aslw@hku.hk | - |
dc.identifier.authority | Wong, SL=rp02139 | - |
dc.identifier.doi | 10.1007/978-1-0716-0892-0_1 | - |
dc.identifier.pmid | 33125641 | - |
dc.identifier.scopus | eid_2-s2.0-85094935016 | - |
dc.identifier.hkuros | 321215 | - |
dc.identifier.volume | 2199 | - |
dc.identifier.spage | 3 | - |
dc.identifier.epage | 12 | - |
dc.publisher.place | United States | - |