File Download
Links for fulltext
(May Require Subscription)
- Publisher Website: 10.1016/j.mex.2020.101190
- Scopus: eid_2-s2.0-85098480587
- PMID: 33425688
- WOS: WOS:000707188500007
- Find via
Supplementary
- Citations:
- Appears in Collections:
Article: The method to dynamically screen and print single cells using microfluidics with pneumatic microvalves
Title | The method to dynamically screen and print single cells using microfluidics with pneumatic microvalves |
---|---|
Authors | |
Keywords | Dynamic screening Printing Microfluidics Pneumatic microvalves Single cells |
Issue Date | 2021 |
Publisher | Elsevier: Creative Commons Licenses. The Journal's web site is located at https://www.journals.elsevier.com/methodsx/ |
Citation | MethodsX, 2021, v. 8, p. article no. 101190 How to Cite? |
Abstract | Printing single cells into individual chambers is of critical importance for single-cell analysis using traditional equipment, for instance, single-cell clonal expansion or sequencing. The size of cells can usually be a reflection of their types, functions, and even cell cycle phases. Therefore, printing individual cells within the desired size range is of essential application potential in single-cell analysis. This paper presents a method for the development of a microfluidic chip integrating pneumatic microvalves to print single cells with appropriate size into standard well plates. The reported method provided essential guidelines for the fabrication of multi-layer microfluidic chips, control of the membrane deflection to screen cell size, and printing of single cells. In brief, this paper reports:
• the manufacturing of the chip using standard soft lithography;
• the protocol to dynamically screen both the lower and the upper size limit of cells passing through the valves by deflection of the valve membrane;
• the screening and dispensing of suspended human umbilical vein endothelial cells (HUVECs) into 384-well plates with high viability. |
Persistent Identifier | http://hdl.handle.net/10722/295461 |
ISSN | 2023 Impact Factor: 1.6 2023 SCImago Journal Rankings: 0.393 |
PubMed Central ID | |
ISI Accession Number ID |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Chen, C | - |
dc.contributor.author | Zhu, Y | - |
dc.contributor.author | Ho, JWK | - |
dc.contributor.author | Chen, H | - |
dc.date.accessioned | 2021-01-25T11:15:15Z | - |
dc.date.available | 2021-01-25T11:15:15Z | - |
dc.date.issued | 2021 | - |
dc.identifier.citation | MethodsX, 2021, v. 8, p. article no. 101190 | - |
dc.identifier.issn | 2215-0161 | - |
dc.identifier.uri | http://hdl.handle.net/10722/295461 | - |
dc.description.abstract | Printing single cells into individual chambers is of critical importance for single-cell analysis using traditional equipment, for instance, single-cell clonal expansion or sequencing. The size of cells can usually be a reflection of their types, functions, and even cell cycle phases. Therefore, printing individual cells within the desired size range is of essential application potential in single-cell analysis. This paper presents a method for the development of a microfluidic chip integrating pneumatic microvalves to print single cells with appropriate size into standard well plates. The reported method provided essential guidelines for the fabrication of multi-layer microfluidic chips, control of the membrane deflection to screen cell size, and printing of single cells. In brief, this paper reports: • the manufacturing of the chip using standard soft lithography; • the protocol to dynamically screen both the lower and the upper size limit of cells passing through the valves by deflection of the valve membrane; • the screening and dispensing of suspended human umbilical vein endothelial cells (HUVECs) into 384-well plates with high viability. | - |
dc.language | eng | - |
dc.publisher | Elsevier: Creative Commons Licenses. The Journal's web site is located at https://www.journals.elsevier.com/methodsx/ | - |
dc.relation.ispartof | MethodsX | - |
dc.rights | This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License. | - |
dc.subject | Dynamic screening | - |
dc.subject | Printing | - |
dc.subject | Microfluidics | - |
dc.subject | Pneumatic microvalves | - |
dc.subject | Single cells | - |
dc.title | The method to dynamically screen and print single cells using microfluidics with pneumatic microvalves | - |
dc.type | Article | - |
dc.identifier.email | Ho, JWK: jwkho@hku.hk | - |
dc.identifier.authority | Ho, JWK=rp02436 | - |
dc.description.nature | published_or_final_version | - |
dc.identifier.doi | 10.1016/j.mex.2020.101190 | - |
dc.identifier.pmid | 33425688 | - |
dc.identifier.pmcid | PMC7779779 | - |
dc.identifier.scopus | eid_2-s2.0-85098480587 | - |
dc.identifier.hkuros | 320992 | - |
dc.identifier.volume | 8 | - |
dc.identifier.spage | article no. 101190 | - |
dc.identifier.epage | article no. 101190 | - |
dc.identifier.isi | WOS:000707188500007 | - |
dc.publisher.place | Netherlands | - |