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Article: A Microfabricated Sandwiching Assay for Nanoliter and High-Throughput Biomarker Screening

TitleA Microfabricated Sandwiching Assay for Nanoliter and High-Throughput Biomarker Screening
Authors
Keywordsdrug screening
high-throughput screening
microwells
micropillars
Issue Date2019
Citation
Small, 2019, v. 15, n. 15, article no. 1900300 How to Cite?
Abstract© 2019 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim Cells secrete substances that are essential to the understanding of numerous immunological phenomena and are extensively used in clinical diagnoses. Countless techniques for screening of biomarker secretion in living cells have generated valuable information on cell function and physiology, but low volume and real-time analysis is a bottleneck for a range of approaches. Here, a simple, highly sensitive assay using a high-throughput micropillar and microwell array chip (MIMIC) platform is presented for monitoring of biomarkers secreted by cancer cells. The sensing element is a micropillar array that uses the enzyme-linked immunosorbent assay (ELISA) mechanism to detect captured biomolecules. When integrated with a microwell array where few cells are localized, interleukin 8 (IL-8) secretion can be monitored with nanoliter volume using multiple micropillar arrays. The trend of cell secretions measured using MIMICs matches the results from conventional ELISA well while it requires orders of magnitude less cells and volumes. Moreover, the proposed MIMIC is examined to be used as a drug screening platform by delivering drugs using micropillar arrays in combination with a microfluidic system and then detecting biomolecules from cells as exposed to drugs.
Persistent Identifierhttp://hdl.handle.net/10722/295430
ISSN
2023 Impact Factor: 13.0
2023 SCImago Journal Rankings: 3.348
ISI Accession Number ID
Errata

 

DC FieldValueLanguage
dc.contributor.authorBandaru, Praveen-
dc.contributor.authorChu, Dafeng-
dc.contributor.authorSun, Wujin-
dc.contributor.authorLasli, Soufian-
dc.contributor.authorZhao, Chuanzhen-
dc.contributor.authorHou, Shuang-
dc.contributor.authorZhang, Shiming-
dc.contributor.authorNi, Jiahua-
dc.contributor.authorCefaloni, Giorgia-
dc.contributor.authorAhadian, Samad-
dc.contributor.authorDokmeci, Mehmet Remzi-
dc.contributor.authorSengupta, Shiladitya-
dc.contributor.authorLee, Junmin-
dc.contributor.authorKhademhosseini, Ali-
dc.date.accessioned2021-01-18T15:46:51Z-
dc.date.available2021-01-18T15:46:51Z-
dc.date.issued2019-
dc.identifier.citationSmall, 2019, v. 15, n. 15, article no. 1900300-
dc.identifier.issn1613-6810-
dc.identifier.urihttp://hdl.handle.net/10722/295430-
dc.description.abstract© 2019 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim Cells secrete substances that are essential to the understanding of numerous immunological phenomena and are extensively used in clinical diagnoses. Countless techniques for screening of biomarker secretion in living cells have generated valuable information on cell function and physiology, but low volume and real-time analysis is a bottleneck for a range of approaches. Here, a simple, highly sensitive assay using a high-throughput micropillar and microwell array chip (MIMIC) platform is presented for monitoring of biomarkers secreted by cancer cells. The sensing element is a micropillar array that uses the enzyme-linked immunosorbent assay (ELISA) mechanism to detect captured biomolecules. When integrated with a microwell array where few cells are localized, interleukin 8 (IL-8) secretion can be monitored with nanoliter volume using multiple micropillar arrays. The trend of cell secretions measured using MIMICs matches the results from conventional ELISA well while it requires orders of magnitude less cells and volumes. Moreover, the proposed MIMIC is examined to be used as a drug screening platform by delivering drugs using micropillar arrays in combination with a microfluidic system and then detecting biomolecules from cells as exposed to drugs.-
dc.languageeng-
dc.relation.ispartofSmall-
dc.subjectdrug screening-
dc.subjecthigh-throughput screening-
dc.subjectmicrowells-
dc.subjectmicropillars-
dc.titleA Microfabricated Sandwiching Assay for Nanoliter and High-Throughput Biomarker Screening-
dc.typeArticle-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1002/smll.201900300-
dc.identifier.pmid30884183-
dc.identifier.scopuseid_2-s2.0-85064192078-
dc.identifier.volume15-
dc.identifier.issue15-
dc.identifier.spagearticle no. 1900300-
dc.identifier.epagearticle no. 1900300-
dc.identifier.eissn1613-6829-
dc.identifier.isiWOS:000467418900006-
dc.relation.erratumdoi:10.1002/smll.201903137-
dc.relation.erratumeid:eid_2-s2.0-85070673743-
dc.identifier.issnl1613-6810-

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