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Article: Fluorescent Probes For In Vitro And In Vivo Quantification of Hydrogen Peroxide

TitleFluorescent Probes For In Vitro And In Vivo Quantification of Hydrogen Peroxide
Authors
Ye, SHu, JJZhao, QAYang, D
Issue Date2020
PublisherRoyal Society of Chemistry: Open Access Journals. The Journal's web site is located at http://www.rsc.org/publishing/journals/sc/About.asp
Citation
Chemical Science, 2020, v. 11 n. 44, p. 11989-11997 How to Cite?
DOI: http://dx.doi.org/10.1039/D0SC04888G
AbstractHydrogen peroxide (H2O2) plays essential roles in redox signaling and oxidative stress, and its dynamic concentration is critical to human health and diseases. Here we report the design, syntheses, and biological applications of HKPerox-Red and HKPerox-Ratio for quantitative measurement of H2O2. Both probes were successfully applied to detect endogenous H2O2 fluxes in living cells or zebrafish, and biological effects of multiple stress inducers including rotenone, arsenic trioxide, and starvation were investigated. As H2O2 is a common by-product for oxidase oxidation, a general assay was developed for ultrasensitive detection of various metabolites (glucose, uric acid, and sarcosine). Moreover, cellular H2O2 measurements were achieved for the first time by combining flow cytometry with live cell calibration. This study provides a pair of unique molecular tools for advanced H2O2 bio-imaging and assay development.
Persistent Identifierhttp://hdl.handle.net/10722/295322
ISSN
2041-6520
2023 Impact Factor: 7.6
2023 SCImago Journal Rankings: 2.333
ISI Accession Number ID
WOS:000590392400025

 

DC FieldValueLanguage
dc.contributor.authorYe, S-
dc.contributor.authorHu, JJ-
dc.contributor.authorZhao, QA-
dc.contributor.authorYang, D-
dc.date.accessioned2021-01-11T13:58:28Z-
dc.date.available2021-01-11T13:58:28Z-
dc.date.issued2020-
dc.identifier.citationChemical Science, 2020, v. 11 n. 44, p. 11989-11997-
dc.identifier.issn2041-6520-
dc.identifier.urihttp://hdl.handle.net/10722/295322-
dc.description.abstractHydrogen peroxide (H2O2) plays essential roles in redox signaling and oxidative stress, and its dynamic concentration is critical to human health and diseases. Here we report the design, syntheses, and biological applications of HKPerox-Red and HKPerox-Ratio for quantitative measurement of H2O2. Both probes were successfully applied to detect endogenous H2O2 fluxes in living cells or zebrafish, and biological effects of multiple stress inducers including rotenone, arsenic trioxide, and starvation were investigated. As H2O2 is a common by-product for oxidase oxidation, a general assay was developed for ultrasensitive detection of various metabolites (glucose, uric acid, and sarcosine). Moreover, cellular H2O2 measurements were achieved for the first time by combining flow cytometry with live cell calibration. This study provides a pair of unique molecular tools for advanced H2O2 bio-imaging and assay development.-
dc.languageeng-
dc.publisherRoyal Society of Chemistry: Open Access Journals. The Journal's web site is located at http://www.rsc.org/publishing/journals/sc/About.asp-
dc.relation.ispartofChemical Science-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.titleFluorescent Probes For In Vitro And In Vivo Quantification of Hydrogen Peroxide-
dc.typeArticle-
dc.identifier.emailYe, S: yesen@hku.hk-
dc.identifier.emailYang, D: yangdan@hku.hk-
dc.identifier.authorityYe, S=rp02805-
dc.identifier.authorityYang, D=rp00825-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1039/D0SC04888G-
dc.identifier.scopuseid_2-s2.0-85096465918-
dc.identifier.hkuros320933-
dc.identifier.volume11-
dc.identifier.issue44-
dc.identifier.spage11989-
dc.identifier.epage11997-
dc.identifier.isiWOS:000590392400025-
dc.publisher.placeUnited Kingdom-

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