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Article: Direct deposition of crystalline aragonite in the controlled biomineralization of the calcareous tubeworm

TitleDirect deposition of crystalline aragonite in the controlled biomineralization of the calcareous tubeworm
Authors
Keywordstubeworms
biomineralization
internal pH
FIB-TEM
calcification
Issue Date2015
PublisherFrontiers Research Foundation. The Journal's web site is located at http://www.frontiersin.org/Marine_Science
Citation
Frontiers in Marine Science, 2015, v. 2 n. Nov, p. article no. 97 How to Cite?
AbstractAlthough space delineation is a well-accepted requirement for biologically controlled biomineralization, the actual location of the mineralizing compartment within marine invertebrates has only recently been determined. We observed that the biomineralization was compartmented within the collar region of the metamorphosing larvae of Hydrodies elegans at its earliest possible time, i.e., at the post-metamorphic stage. We have also found that these highly regulated compartments contained aragonite crystals, as detected by EBSD and confirmed by electron diffraction TEM. Within these compartments, the metamorphosed larvae maintained a pH 9, at the pKa for CaCO3 formation. This model describes how biomineralization is a space delineation event in which calcium carbonate formation is an intracellular phenomenon. Significance statement: Using the tubeworm, a popular invertebrate model for marine research, we propose a unifying mechanism of calcification that emphasizes the importance of specialized mineralizing compartments in providing delineated space for controlled biomineralization within a multicellular organism. Through the use of correlative microscopy, we have demonstrated the functional observation of organisms under fluorescent microscopy can be directly followed by performing structural analysis using the extreme edge of electron microscopy, specifically SEM-EBSD and FIB-TEM. Unlike conventional fluorescent observations that require the use of fixation artifacts that are inevitable in electron microscopy, this novel method of understanding both the function and structure of any calcifying organism avoids such artifacts, while still providing a higher structural resolution.
Persistent Identifierhttp://hdl.handle.net/10722/294633
ISSN
2023 Impact Factor: 2.8
2023 SCImago Journal Rankings: 0.907
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorChan, VBS-
dc.contributor.authorToyofuku, T-
dc.contributor.authorWetzel, G-
dc.contributor.authorSaraf, L-
dc.contributor.authorVengatesen, T-
dc.contributor.authorMount, AS-
dc.date.accessioned2020-12-08T07:39:43Z-
dc.date.available2020-12-08T07:39:43Z-
dc.date.issued2015-
dc.identifier.citationFrontiers in Marine Science, 2015, v. 2 n. Nov, p. article no. 97-
dc.identifier.issn2296-7745-
dc.identifier.urihttp://hdl.handle.net/10722/294633-
dc.description.abstractAlthough space delineation is a well-accepted requirement for biologically controlled biomineralization, the actual location of the mineralizing compartment within marine invertebrates has only recently been determined. We observed that the biomineralization was compartmented within the collar region of the metamorphosing larvae of Hydrodies elegans at its earliest possible time, i.e., at the post-metamorphic stage. We have also found that these highly regulated compartments contained aragonite crystals, as detected by EBSD and confirmed by electron diffraction TEM. Within these compartments, the metamorphosed larvae maintained a pH 9, at the pKa for CaCO3 formation. This model describes how biomineralization is a space delineation event in which calcium carbonate formation is an intracellular phenomenon. Significance statement: Using the tubeworm, a popular invertebrate model for marine research, we propose a unifying mechanism of calcification that emphasizes the importance of specialized mineralizing compartments in providing delineated space for controlled biomineralization within a multicellular organism. Through the use of correlative microscopy, we have demonstrated the functional observation of organisms under fluorescent microscopy can be directly followed by performing structural analysis using the extreme edge of electron microscopy, specifically SEM-EBSD and FIB-TEM. Unlike conventional fluorescent observations that require the use of fixation artifacts that are inevitable in electron microscopy, this novel method of understanding both the function and structure of any calcifying organism avoids such artifacts, while still providing a higher structural resolution.-
dc.languageeng-
dc.publisherFrontiers Research Foundation. The Journal's web site is located at http://www.frontiersin.org/Marine_Science-
dc.relation.ispartofFrontiers in Marine Science-
dc.rightsThis Document is Protected by copyright and was first published by Frontiers. All rights reserved. It is reproduced with permission.-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.subjecttubeworms-
dc.subjectbiomineralization-
dc.subjectinternal pH-
dc.subjectFIB-TEM-
dc.subjectcalcification-
dc.titleDirect deposition of crystalline aragonite in the controlled biomineralization of the calcareous tubeworm-
dc.typeArticle-
dc.identifier.emailVengatesen, T: rajan@hkucc.hku.hk-
dc.identifier.authorityVengatesen, T=rp00796-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.3389/fmars.2015.00097-
dc.identifier.scopuseid_2-s2.0-85008714416-
dc.identifier.hkuros320375-
dc.identifier.volume2-
dc.identifier.issueNov-
dc.identifier.spagearticle no. 97-
dc.identifier.epagearticle no. 97-
dc.identifier.isiWOS:000485324300097-
dc.publisher.placeSwitzerland-
dc.identifier.issnl2296-7745-

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