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Article: Combination of Isoflurane and Propofol as General Anesthesia During Orthopedic Surgery of Perioperative Cerebral Hypoperfusion Rats to Avoid Cognitive Impairment

TitleCombination of Isoflurane and Propofol as General Anesthesia During Orthopedic Surgery of Perioperative Cerebral Hypoperfusion Rats to Avoid Cognitive Impairment
Authors
Keywordscerebral hypoperfusion
cognitive function
isoflurane
propofol
GABA(A)R α
Issue Date2020
PublisherFrontiers. The Journal's web site is located at http://www.frontiersin.org/Medicine
Citation
Frontiers in Medicine, 2020, v. 7, p. article no. 549081 How to Cite?
AbstractBackground: Perioperative cerebral hypoperfusion (CH) is common, although the underlying mechanism of cognitive impairment that results due to perioperative cerebral hypoperfusion remains to be determined. Isoflurane anesthesia induces neuronal injury via endoplasmic reticulum (ER) stress, whereas a sub-anesthetic dose of propofol improves postoperative cognitive function. However, the effects of the combination of isoflurane plus propofol, which is a common aesthetic combination administered to patients, on ER stress and cognition remain unknown. Methods: We sought to determine the effects of isoflurane plus propofol on ER stress and cognitive function in rats insulted by cerebral hypoperfusion. Ligation of the bilateral common carotid arteries (CCA) was adopted to develop the cerebral hypoperfusion rat model. A second surgery, open reduction and internal fixation (ORIF), requiring general anesthesia, was performed 30 days later so that the effects of anesthetics on the cognitive function of CH rats could be assessed. Rats received isoflurane alone (1.9%), propofol alone (40 mg center dot kg(-1)center dot h(-1)) or a combination of isoflurane and propofol (1% and 20 mg center dot kg(-1)center dot h(-1) or 1.4% and 10 mg center dot kg(-1)center dot h(-1)). Behavioral studies (contextual fear conditioning [FC] test), histological analyses (Nissl staining) and biochemical analyses (western blotting of the harvested rat brain tissues) were employed. Results: Hippocampus-dependent memory of rats in group IP1 (1% isoflurane plus 20 mg center dot kg(-1)center dot h(-1) propofol) was not impaired, and expression level of gamma-aminobutyric acid A type receptor alpha 1 subunit, a key cognition-related protein, remained normal. ER stress alleviator, binding immunoglobulin protein, increased extremely while ER stress transcription factor, C/EBP homologous protein, showed no statistical difference compared with the control group. Numbers of surviving neurons confirmed the substantial neuronal damage caused by propofol or isoflurane alone. Conclusions: These data suggest that ER stress contributes to the underlying mechanism of cognitive impairment and that the combination of isoflurane and propofol did not aggravate cognitive impairment and ER stress in aging rats with CH that were further subjected to ORIF surgery.
Persistent Identifierhttp://hdl.handle.net/10722/294616
ISSN
2021 Impact Factor: 5.058
2020 SCImago Journal Rankings: 1.388
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorBu, X-
dc.contributor.authorLi, T-
dc.contributor.authorWang, H-
dc.contributor.authorXia, Z-
dc.contributor.authorGuo, D-
dc.contributor.authorWang, J-
dc.contributor.authorSun, Y-
dc.contributor.authorYang, C-
dc.contributor.authorLiu, G-
dc.contributor.authorMa, J-
dc.contributor.authorYang, Z-
dc.contributor.authorWang, G-
dc.date.accessioned2020-12-08T07:39:29Z-
dc.date.available2020-12-08T07:39:29Z-
dc.date.issued2020-
dc.identifier.citationFrontiers in Medicine, 2020, v. 7, p. article no. 549081-
dc.identifier.issn2296-858X-
dc.identifier.urihttp://hdl.handle.net/10722/294616-
dc.description.abstractBackground: Perioperative cerebral hypoperfusion (CH) is common, although the underlying mechanism of cognitive impairment that results due to perioperative cerebral hypoperfusion remains to be determined. Isoflurane anesthesia induces neuronal injury via endoplasmic reticulum (ER) stress, whereas a sub-anesthetic dose of propofol improves postoperative cognitive function. However, the effects of the combination of isoflurane plus propofol, which is a common aesthetic combination administered to patients, on ER stress and cognition remain unknown. Methods: We sought to determine the effects of isoflurane plus propofol on ER stress and cognitive function in rats insulted by cerebral hypoperfusion. Ligation of the bilateral common carotid arteries (CCA) was adopted to develop the cerebral hypoperfusion rat model. A second surgery, open reduction and internal fixation (ORIF), requiring general anesthesia, was performed 30 days later so that the effects of anesthetics on the cognitive function of CH rats could be assessed. Rats received isoflurane alone (1.9%), propofol alone (40 mg center dot kg(-1)center dot h(-1)) or a combination of isoflurane and propofol (1% and 20 mg center dot kg(-1)center dot h(-1) or 1.4% and 10 mg center dot kg(-1)center dot h(-1)). Behavioral studies (contextual fear conditioning [FC] test), histological analyses (Nissl staining) and biochemical analyses (western blotting of the harvested rat brain tissues) were employed. Results: Hippocampus-dependent memory of rats in group IP1 (1% isoflurane plus 20 mg center dot kg(-1)center dot h(-1) propofol) was not impaired, and expression level of gamma-aminobutyric acid A type receptor alpha 1 subunit, a key cognition-related protein, remained normal. ER stress alleviator, binding immunoglobulin protein, increased extremely while ER stress transcription factor, C/EBP homologous protein, showed no statistical difference compared with the control group. Numbers of surviving neurons confirmed the substantial neuronal damage caused by propofol or isoflurane alone. Conclusions: These data suggest that ER stress contributes to the underlying mechanism of cognitive impairment and that the combination of isoflurane and propofol did not aggravate cognitive impairment and ER stress in aging rats with CH that were further subjected to ORIF surgery.-
dc.languageeng-
dc.publisherFrontiers. The Journal's web site is located at http://www.frontiersin.org/Medicine-
dc.relation.ispartofFrontiers in Medicine-
dc.rightsThis Document is Protected by copyright and was first published by Frontiers. All rights reserved. It is reproduced with permission.-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.subjectcerebral hypoperfusion-
dc.subjectcognitive function-
dc.subjectisoflurane-
dc.subjectpropofol-
dc.subjectGABA(A)R α-
dc.titleCombination of Isoflurane and Propofol as General Anesthesia During Orthopedic Surgery of Perioperative Cerebral Hypoperfusion Rats to Avoid Cognitive Impairment-
dc.typeArticle-
dc.identifier.emailXia, Z: zyxia@hkucc.hku.hk-
dc.identifier.authorityXia, Z=rp00532-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.3389/fmed.2020.549081-
dc.identifier.pmid33195298-
dc.identifier.pmcidPMC7646644-
dc.identifier.scopuseid_2-s2.0-85096923360-
dc.identifier.hkuros320395-
dc.identifier.volume7-
dc.identifier.spagearticle no. 549081-
dc.identifier.epagearticle no. 549081-
dc.identifier.isiWOS:000586800600001-
dc.publisher.placeSwitzerland-

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