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Article: A human-murine chimera model for in utero human hematopoietic stem cell transplantation

TitleA human-murine chimera model for in utero human hematopoietic stem cell transplantation
Authors
KeywordsMice-SCID
Hematopoietic stem cell
Human in utero transplantation
Biological assay
Issue Date1999
Citation
Biology of Blood and Marrow Transplantation, 1999, v. 5, n. 1, p. 1-7 How to Cite?
AbstractTo date, 16 in utero hematopoietic stem cell (HSC) transplants for diseases other than immunodeficiency disorders have been reported. No therapeutic level of engraftment was detected in 15 of these transplants. To overcome engraftment failure, we transplanted a very large number (5 billion paternal CD34+ cells/kg) of HSCs to a fetus with leukodystrophy during the first trimester of gestation. As reported previously, the fetus died in utero 7 weeks after the procedure and the cause of death appeared to be overwhelming donor engraftment. In the present investigation, we developed a human-murine chimera model to test for the optimal donor cell dose for human in utero transplantation. We found a strong correlation between the level of donor engraftment in three human fetuses transplanted for leukodystrophy during the first trimester of gestation and the results of parallel xenotransplants of the same human donor cells using the NOD/SCID mouse model. This small animal model appears to predict both extremes of hyperengraftment (seen in the first human fetus transplanted) and engraftment failure (seen in the second and third human fetuses transplanted in utero). These and future correlated clinical and laboratory assay results may be useful for the development of in utero transplants for a variety of congenital disorders.
Persistent Identifierhttp://hdl.handle.net/10722/294523
ISSN
2022 Impact Factor: 4.3
2020 SCImago Journal Rankings: 2.301

 

DC FieldValueLanguage
dc.contributor.authorLeung, Wing-
dc.contributor.authorBlakemore, Karin-
dc.contributor.authorJones, Richard J.-
dc.contributor.authorMoser, Hugo W.-
dc.contributor.authorMukherjee, Goutam-
dc.contributor.authorGriffin, Constance A.-
dc.contributor.authorRosenblum-Vos, Lynne S.-
dc.contributor.authorCivin, Curt I.-
dc.date.accessioned2020-12-03T08:22:55Z-
dc.date.available2020-12-03T08:22:55Z-
dc.date.issued1999-
dc.identifier.citationBiology of Blood and Marrow Transplantation, 1999, v. 5, n. 1, p. 1-7-
dc.identifier.issn1083-8791-
dc.identifier.urihttp://hdl.handle.net/10722/294523-
dc.description.abstractTo date, 16 in utero hematopoietic stem cell (HSC) transplants for diseases other than immunodeficiency disorders have been reported. No therapeutic level of engraftment was detected in 15 of these transplants. To overcome engraftment failure, we transplanted a very large number (5 billion paternal CD34+ cells/kg) of HSCs to a fetus with leukodystrophy during the first trimester of gestation. As reported previously, the fetus died in utero 7 weeks after the procedure and the cause of death appeared to be overwhelming donor engraftment. In the present investigation, we developed a human-murine chimera model to test for the optimal donor cell dose for human in utero transplantation. We found a strong correlation between the level of donor engraftment in three human fetuses transplanted for leukodystrophy during the first trimester of gestation and the results of parallel xenotransplants of the same human donor cells using the NOD/SCID mouse model. This small animal model appears to predict both extremes of hyperengraftment (seen in the first human fetus transplanted) and engraftment failure (seen in the second and third human fetuses transplanted in utero). These and future correlated clinical and laboratory assay results may be useful for the development of in utero transplants for a variety of congenital disorders.-
dc.languageeng-
dc.relation.ispartofBiology of Blood and Marrow Transplantation-
dc.subjectMice-SCID-
dc.subjectHematopoietic stem cell-
dc.subjectHuman in utero transplantation-
dc.subjectBiological assay-
dc.titleA human-murine chimera model for in utero human hematopoietic stem cell transplantation-
dc.typeArticle-
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1053/bbmt.1999.v5.pm10232735-
dc.identifier.pmid10232735-
dc.identifier.scopuseid_2-s2.0-0032617681-
dc.identifier.volume5-
dc.identifier.issue1-
dc.identifier.spage1-
dc.identifier.epage7-
dc.identifier.issnl1083-8791-

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