File Download
There are no files associated with this item.
Links for fulltext
(May Require Subscription)
- Publisher Website: 10.1016/j.canlet.2015.07.042
- Scopus: eid_2-s2.0-84940467289
- PMID: 26276724
- WOS: WOS:000361265500008
- Find via
Supplementary
- Citations:
- Appears in Collections:
Article: Activated and expanded natural killer cells target osteosarcoma tumor initiating cells in an NKG2D-NKG2DL dependent manner
Title | Activated and expanded natural killer cells target osteosarcoma tumor initiating cells in an NKG2D-NKG2DL dependent manner |
---|---|
Authors | |
Keywords | Osteosarcoma tumor initiating cells NKG2D-NKG2DL interactions Natural killer cells Immunotherapy |
Issue Date | 2015 |
Citation | Cancer Letters, 2015, v. 368, n. 1, p. 54-63 How to Cite? |
Abstract | © 2015 Elsevier Ireland Ltd. Current therapies fail to cure most metastatic or recurrent bone cancer. We explored the efficacy and the pathways involved in natural killer (NK) cells' elimination of osteosarcoma (OS) cells, including tumor initiating cells (TICs), which are responsible for chemotherapy resistance, recurrence, and metastasis.The expression of ligands for NK cell receptors was studied in primary OS cell lines by flow cytometry. In vitro cytotoxicity of activated and expanded NK (NKAE) cells against OS was tested, and the pathways involved explored by using specific antibody blockade. NKAE cells' ability to target OS TICs was analyzed by flow cytometry and sphere formation assays. Spironolactone (SPIR) was tested for its ability to increase OS cells' susceptibility to NK cell lysis in vitro and in vivo.We found OS cells were susceptible to NKAE cells' lysis both in vivo and in vitro, and this cytolytic activity relied on interaction between NKG2D receptor and NKG2D ligands (NKG2DL). SPIR increased OS cells' susceptibility to lysis by NKAE cells, and could shrink the OS TICs.Our results show NKAE cells target OS cells including the TICs compartment, supporting the use of NK-cell based immunotherapies for OS. |
Persistent Identifier | http://hdl.handle.net/10722/294482 |
ISSN | 2023 Impact Factor: 9.1 2023 SCImago Journal Rankings: 2.595 |
ISI Accession Number ID |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Fernández, L. | - |
dc.contributor.author | Valentín, J. | - |
dc.contributor.author | Zalacain, M. | - |
dc.contributor.author | Leung, W. | - |
dc.contributor.author | Patiño-García, A. | - |
dc.contributor.author | Pérez-Martínez, A. | - |
dc.date.accessioned | 2020-12-03T08:22:50Z | - |
dc.date.available | 2020-12-03T08:22:50Z | - |
dc.date.issued | 2015 | - |
dc.identifier.citation | Cancer Letters, 2015, v. 368, n. 1, p. 54-63 | - |
dc.identifier.issn | 0304-3835 | - |
dc.identifier.uri | http://hdl.handle.net/10722/294482 | - |
dc.description.abstract | © 2015 Elsevier Ireland Ltd. Current therapies fail to cure most metastatic or recurrent bone cancer. We explored the efficacy and the pathways involved in natural killer (NK) cells' elimination of osteosarcoma (OS) cells, including tumor initiating cells (TICs), which are responsible for chemotherapy resistance, recurrence, and metastasis.The expression of ligands for NK cell receptors was studied in primary OS cell lines by flow cytometry. In vitro cytotoxicity of activated and expanded NK (NKAE) cells against OS was tested, and the pathways involved explored by using specific antibody blockade. NKAE cells' ability to target OS TICs was analyzed by flow cytometry and sphere formation assays. Spironolactone (SPIR) was tested for its ability to increase OS cells' susceptibility to NK cell lysis in vitro and in vivo.We found OS cells were susceptible to NKAE cells' lysis both in vivo and in vitro, and this cytolytic activity relied on interaction between NKG2D receptor and NKG2D ligands (NKG2DL). SPIR increased OS cells' susceptibility to lysis by NKAE cells, and could shrink the OS TICs.Our results show NKAE cells target OS cells including the TICs compartment, supporting the use of NK-cell based immunotherapies for OS. | - |
dc.language | eng | - |
dc.relation.ispartof | Cancer Letters | - |
dc.subject | Osteosarcoma tumor initiating cells | - |
dc.subject | NKG2D-NKG2DL interactions | - |
dc.subject | Natural killer cells | - |
dc.subject | Immunotherapy | - |
dc.title | Activated and expanded natural killer cells target osteosarcoma tumor initiating cells in an NKG2D-NKG2DL dependent manner | - |
dc.type | Article | - |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1016/j.canlet.2015.07.042 | - |
dc.identifier.pmid | 26276724 | - |
dc.identifier.scopus | eid_2-s2.0-84940467289 | - |
dc.identifier.volume | 368 | - |
dc.identifier.issue | 1 | - |
dc.identifier.spage | 54 | - |
dc.identifier.epage | 63 | - |
dc.identifier.eissn | 1872-7980 | - |
dc.identifier.isi | WOS:000361265500008 | - |
dc.identifier.issnl | 0304-3835 | - |