File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Quantity and quality of engrafting cells in cord blood and autologous mobilized peripheral blood

TitleQuantity and quality of engrafting cells in cord blood and autologous mobilized peripheral blood
Authors
KeywordsChimera assays
Hematopoiesis
Stem cells
CD34
Bone marrow transplantation
Issue Date1999
Citation
Biology of Blood and Marrow Transplantation, 1999, v. 5, n. 2, p. 69-76 How to Cite?
AbstractCord blood (CB) and autologous mobilized peripheral blood stem/progenitor cells (PBSC) are now used widely for clinical transplantation. We characterized the short-term (<8 weeks) and long-term (>8 weeks) engraftment in NOD/SCID mice resulting from transplanted CD34+ cells from these two sources. We also quantified the frequency of long-term engrafting cells, and the average proliferative capacity of individual engrafting cells by a competitive repopulation assay with binomial variance-covariance modeling. We found that 0.5 million CD34+ CB cells were able to generate sustained, high-level, multilineage human hematopoiesis, whereas a sixfold higher number of CD34+ PBSC (3 million) from cancer patients undergoing chemotherapy generated comparable short-term, but much lower sustained multilineage human hematopoiesis after transplantation. In comparison to CD34+ cells from PBSC from cancer patients, long-term engrafting cells were approximately eightfold enriched in CB CD34+ cells, and each CB long-term engrafting cell had an ∼15-fold higher multilineage proliferative capacity. Thus, the number and function of transplantable hematopoietic cells were remarkably different between these two sources of stem/progenitor cells.
Persistent Identifierhttp://hdl.handle.net/10722/294387
ISSN
2022 Impact Factor: 4.3
2020 SCImago Journal Rankings: 2.301

 

DC FieldValueLanguage
dc.contributor.authorLeung, Wing-
dc.contributor.authorRamírez, Manuel-
dc.contributor.authorCivin, Curt I.-
dc.date.accessioned2020-12-03T08:22:37Z-
dc.date.available2020-12-03T08:22:37Z-
dc.date.issued1999-
dc.identifier.citationBiology of Blood and Marrow Transplantation, 1999, v. 5, n. 2, p. 69-76-
dc.identifier.issn1083-8791-
dc.identifier.urihttp://hdl.handle.net/10722/294387-
dc.description.abstractCord blood (CB) and autologous mobilized peripheral blood stem/progenitor cells (PBSC) are now used widely for clinical transplantation. We characterized the short-term (<8 weeks) and long-term (>8 weeks) engraftment in NOD/SCID mice resulting from transplanted CD34+ cells from these two sources. We also quantified the frequency of long-term engrafting cells, and the average proliferative capacity of individual engrafting cells by a competitive repopulation assay with binomial variance-covariance modeling. We found that 0.5 million CD34+ CB cells were able to generate sustained, high-level, multilineage human hematopoiesis, whereas a sixfold higher number of CD34+ PBSC (3 million) from cancer patients undergoing chemotherapy generated comparable short-term, but much lower sustained multilineage human hematopoiesis after transplantation. In comparison to CD34+ cells from PBSC from cancer patients, long-term engrafting cells were approximately eightfold enriched in CB CD34+ cells, and each CB long-term engrafting cell had an ∼15-fold higher multilineage proliferative capacity. Thus, the number and function of transplantable hematopoietic cells were remarkably different between these two sources of stem/progenitor cells.-
dc.languageeng-
dc.relation.ispartofBiology of Blood and Marrow Transplantation-
dc.subjectChimera assays-
dc.subjectHematopoiesis-
dc.subjectStem cells-
dc.subjectCD34-
dc.subjectBone marrow transplantation-
dc.titleQuantity and quality of engrafting cells in cord blood and autologous mobilized peripheral blood-
dc.typeArticle-
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1053/bbmt.1999.v5.pm10371358-
dc.identifier.pmid10371358-
dc.identifier.scopuseid_2-s2.0-0032603279-
dc.identifier.volume5-
dc.identifier.issue2-
dc.identifier.spage69-
dc.identifier.epage76-
dc.identifier.issnl1083-8791-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats