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Article: A novel incompatibility group X3 plasmid carrying blaNDM-1 encodes a small RNA that regulates host fucose metabolism and biofilm formation

TitleA novel incompatibility group X3 plasmid carrying blaNDM-1 encodes a small RNA that regulates host fucose metabolism and biofilm formation
Authors
KeywordsDrug resistance
MDR
plasmid
NDM1
RNA-seq
Issue Date2020
PublisherTaylor & Francis Inc. The Journal's web site is located at http://www.tandfonline.com/krnb
Citation
RNA Biology, 2020, v. 17 n. 12, p. 1767-1776 How to Cite?
AbstractThe emergence of New Delhi metallo-beta-lactamase (NDM-1) has become a major health threat to clinical managements of gram-negative bacteria infections. A novel incompatibility group X3 plasmid (IncX3) pNDM-HN380 carrying bla (NDM-1) has recently been found to epidemiologically link with multiple geographical areas in China. In this paper, we studied the metabolic responses of host bacteria E. coli J53 upon introduction of pNDM-HN380. A reduction of bacterial motility was observed in J53/pNDM-HN380. We profiled the RNA repertoires of the transconjugants and found a downregulation of genes involved in flagella and chemotaxis metabolic pathways at logarithmic (log) phase. We also identified a novel intragenic region (IGR) small RNA plas2. The plasmid-transcribed sRNA IGR plas2 was further characterized as a regulator of fucRwhich controls the fucose metabolism. By knockdown of IGR plas2 using an antisense decoy, we managed to inhibit the formation of bacterial biofilm of the host. Our study demonstrated a potential way of utilizing plasmid-transcribed sRNA against infectious bacteria.
Persistent Identifierhttp://hdl.handle.net/10722/294114
ISSN
2023 Impact Factor: 3.6
2023 SCImago Journal Rankings: 1.510
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorHuang, C-
dc.contributor.authorLiu, LZ-
dc.contributor.authorKong, HK-
dc.contributor.authorLaw, COK-
dc.contributor.authorHoa, PQ-
dc.contributor.authorHo, PL-
dc.contributor.authorLau, TCK-
dc.date.accessioned2020-11-23T08:26:31Z-
dc.date.available2020-11-23T08:26:31Z-
dc.date.issued2020-
dc.identifier.citationRNA Biology, 2020, v. 17 n. 12, p. 1767-1776-
dc.identifier.issn1547-6286-
dc.identifier.urihttp://hdl.handle.net/10722/294114-
dc.description.abstractThe emergence of New Delhi metallo-beta-lactamase (NDM-1) has become a major health threat to clinical managements of gram-negative bacteria infections. A novel incompatibility group X3 plasmid (IncX3) pNDM-HN380 carrying bla (NDM-1) has recently been found to epidemiologically link with multiple geographical areas in China. In this paper, we studied the metabolic responses of host bacteria E. coli J53 upon introduction of pNDM-HN380. A reduction of bacterial motility was observed in J53/pNDM-HN380. We profiled the RNA repertoires of the transconjugants and found a downregulation of genes involved in flagella and chemotaxis metabolic pathways at logarithmic (log) phase. We also identified a novel intragenic region (IGR) small RNA plas2. The plasmid-transcribed sRNA IGR plas2 was further characterized as a regulator of fucRwhich controls the fucose metabolism. By knockdown of IGR plas2 using an antisense decoy, we managed to inhibit the formation of bacterial biofilm of the host. Our study demonstrated a potential way of utilizing plasmid-transcribed sRNA against infectious bacteria.-
dc.languageeng-
dc.publisherTaylor & Francis Inc. The Journal's web site is located at http://www.tandfonline.com/krnb-
dc.relation.ispartofRNA Biology-
dc.rightsThis is an Accepted Manuscript of an article published by Taylor & Francis in [JOURNAL TITLE] on [date of publication], available online: http://www.tandfonline.com/[Article DOI].-
dc.subjectDrug resistance-
dc.subjectMDR-
dc.subjectplasmid-
dc.subjectNDM1-
dc.subjectRNA-seq-
dc.titleA novel incompatibility group X3 plasmid carrying blaNDM-1 encodes a small RNA that regulates host fucose metabolism and biofilm formation-
dc.typeArticle-
dc.identifier.emailHo, PL: plho@hku.hk-
dc.identifier.authorityHo, PL=rp00406-
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1080/15476286.2020.1780040-
dc.identifier.pmid32594845-
dc.identifier.pmcidPMC7714467-
dc.identifier.scopuseid_2-s2.0-85087500366-
dc.identifier.hkuros319292-
dc.identifier.volume17-
dc.identifier.issue12-
dc.identifier.spage1767-
dc.identifier.epage1776-
dc.identifier.isiWOS:000549501500001-
dc.publisher.placeUnited States-
dc.identifier.issnl1547-6286-

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