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Article: Combination of matrix solid phase dispersion and response surface evaluation for simultaneous detections of multiple bioactive constituents of Traditional Chinese Medicine Formula: Using Baoyuan Capsule as an example

TitleCombination of matrix solid phase dispersion and response surface evaluation for simultaneous detections of multiple bioactive constituents of Traditional Chinese Medicine Formula: Using Baoyuan Capsule as an example
Authors
KeywordsTraditional Chinese medicine
Bioactive constituents
Quality control
Solid phase extraction
Response surface evaluation
Issue Date2020
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jpba
Citation
Journal of Pharmaceutical and Biomedical Analysis, 2020, v. 190, p. article no. 113495 How to Cite?
AbstractTraditional Chinese Medicine (TCM) formulae contain abundant chemical ingredients, leading to the development of high quality TCM productions be difficult. With different pharmacokinetic and pharmacodynamic parameters of the chemical ingredients in a TCM formula, it is desirable to simultaneously identify multiple ingredients for accurate quality control. In the present study, we introduce a novel strategy for TCM formula quality assessment by using the combined methods of the extraction condition optimization, quantitative analyzation, and response surface evaluation. We used Bao-Yuan Capsule (BYC), a patented TCM production, as a model system for quality assessment. We applied matrix solid phase dispersion (MSPD) as a rapid and efficient method to prepare sample extraction. Q-Trap-MS related accurate methods were applied to simultaneously analyze 13 bioactive constituents as bioactive markers in the BYC. Those methods revealed a high sensitivity to detect the target compounds at the concentrations ranging from 0.12 to 0.95 ng/mL for flavonoids and ginsenosides whose recoveries were ranged from 91.93%–105.84%. We employed the response surface methodology to optimize the extraction conditions including dispersant/sample ratio, solvent concentration, and elution volume based on the content of ginsenosides as the test samples. The results showed the high extraction efficiency of ginsenosides with dispersant/sample ratio at 3/4, methanol concentration at 85 %, and elution volume at 15 mL. Taken together, we conclude that the combination strategy of MSPD and response surface evaluation for simultaneous detections of multiple bioactive constituents could be a powerful and efficient method for performing accurately quality control of complex TCM production preparations.
Persistent Identifierhttp://hdl.handle.net/10722/293950
ISSN
2023 Impact Factor: 3.1
2023 SCImago Journal Rankings: 0.584
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorDU, Q-
dc.contributor.authorDeng, R-
dc.contributor.authorGao, C-
dc.contributor.authorShen, J-
dc.date.accessioned2020-11-23T08:24:12Z-
dc.date.available2020-11-23T08:24:12Z-
dc.date.issued2020-
dc.identifier.citationJournal of Pharmaceutical and Biomedical Analysis, 2020, v. 190, p. article no. 113495-
dc.identifier.issn0731-7085-
dc.identifier.urihttp://hdl.handle.net/10722/293950-
dc.description.abstractTraditional Chinese Medicine (TCM) formulae contain abundant chemical ingredients, leading to the development of high quality TCM productions be difficult. With different pharmacokinetic and pharmacodynamic parameters of the chemical ingredients in a TCM formula, it is desirable to simultaneously identify multiple ingredients for accurate quality control. In the present study, we introduce a novel strategy for TCM formula quality assessment by using the combined methods of the extraction condition optimization, quantitative analyzation, and response surface evaluation. We used Bao-Yuan Capsule (BYC), a patented TCM production, as a model system for quality assessment. We applied matrix solid phase dispersion (MSPD) as a rapid and efficient method to prepare sample extraction. Q-Trap-MS related accurate methods were applied to simultaneously analyze 13 bioactive constituents as bioactive markers in the BYC. Those methods revealed a high sensitivity to detect the target compounds at the concentrations ranging from 0.12 to 0.95 ng/mL for flavonoids and ginsenosides whose recoveries were ranged from 91.93%–105.84%. We employed the response surface methodology to optimize the extraction conditions including dispersant/sample ratio, solvent concentration, and elution volume based on the content of ginsenosides as the test samples. The results showed the high extraction efficiency of ginsenosides with dispersant/sample ratio at 3/4, methanol concentration at 85 %, and elution volume at 15 mL. Taken together, we conclude that the combination strategy of MSPD and response surface evaluation for simultaneous detections of multiple bioactive constituents could be a powerful and efficient method for performing accurately quality control of complex TCM production preparations.-
dc.languageeng-
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jpba-
dc.relation.ispartofJournal of Pharmaceutical and Biomedical Analysis-
dc.subjectTraditional Chinese medicine-
dc.subjectBioactive constituents-
dc.subjectQuality control-
dc.subjectSolid phase extraction-
dc.subjectResponse surface evaluation-
dc.titleCombination of matrix solid phase dispersion and response surface evaluation for simultaneous detections of multiple bioactive constituents of Traditional Chinese Medicine Formula: Using Baoyuan Capsule as an example-
dc.typeArticle-
dc.identifier.emailShen, J: shenjg@hku.hk-
dc.identifier.authorityShen, J=rp00487-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.jpba.2020.113495-
dc.identifier.pmid32795779-
dc.identifier.scopuseid_2-s2.0-85089201353-
dc.identifier.hkuros319894-
dc.identifier.volume190-
dc.identifier.spagearticle no. 113495-
dc.identifier.epagearticle no. 113495-
dc.identifier.isiWOS:000573429300019-
dc.publisher.placeNetherlands-
dc.identifier.issnl0731-7085-

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