File Download
There are no files associated with this item.
Links for fulltext
(May Require Subscription)
- Publisher Website: 10.3168/jds.2019-17439
- Scopus: eid_2-s2.0-85085567837
- PMID: 32475665
- WOS: WOS:000550190500010
- Find via
Supplementary
- Citations:
- Appears in Collections:
Article: Structural characterization of exopolysaccharide from Streptococcus thermophilus ASCC 1275
Title | Structural characterization of exopolysaccharide from Streptococcus thermophilus ASCC 1275 |
---|---|
Authors | |
Keywords | exopolysaccharide structure glycosyl linkage nuclear magnetic resonance (NMR) |
Issue Date | 2020 |
Publisher | Elsevier, published in association with American Dairy Science Association. The Journal's web site is located at http://www.journalofdairyscience.org/ |
Citation | Journal of Dairy Science, 2020, v. 103 n. 8, p. 6830-6842 How to Cite? |
Abstract | In this study, we purified and characterized exopolysaccharide (EPS) produced by a high-EPS-producing dairy starter bacterium, Streptococcus thermophilus ASCC 1275. Crude EPS was extracted from S. thermophilus ASCC 1275 and partially purified using dialysis. Further purification and fractionation of exopolysaccharide was conducted using HPLC on a Superose 6 column (Cytiva/Global Life Sciences Solutions, Marlborough, MA). Glycosyl composition analysis, linkage analysis along with 1-dimensional and 2-dimensional nuclear magnetic resonance spectroscopy were performed to deduce the structure of EPS. Three fractions (F) obtained from gel permeation chromatography were termed F1 (2.6%), F2 (45.8%), and F3 (51.6%) with average molecular weights of approximately 511, 40, and 5 kDa, respectively. Monosaccharide composition analysis revealed the dominance of glucose, galactose, and mannose in all 3 fractions. Major linkages observed in F3 were terminal galactopyranosyl (t-Gal), 3-linked glucopyranosyl (3-Glc), 3-linked galactofuranosyl (3-Galf), and 3,6-linked glucopyranosyl (3,6-Glc) and major linkages present in F2 were 4-Glc (48 mol%), followed by terminal mannopyranosyl (t-Man), 2- + 3-linked mannopyranosyl (2-Man+3-Man), and 2,6-linked mannopyranosyl (2,6-Man; total ∼28 mol%). The 1-dimensional and 2-dimensional nuclear magnetic resonance spectroscopy revealed that F2 comprised mannans linked by (1→2) linkages and F3 consisted of linear chains of α-d-glucopyranosyl (α-d-Glcp), β-d-glucopyranosyl (β-d-Glcp), and β-d-galactofuranosyl (β-d-Galf) connected by (1→3) linkages; branching was through (1→6) linkage in F3. A possible structure of EPS in F2 and F3 was proposed. |
Description | Bronze open access |
Persistent Identifier | http://hdl.handle.net/10722/293782 |
ISSN | 2021 Impact Factor: 4.225 2020 SCImago Journal Rankings: 1.483 |
ISI Accession Number ID |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Padmanabhan, A | - |
dc.contributor.author | Shah, NP | - |
dc.date.accessioned | 2020-11-23T08:21:43Z | - |
dc.date.available | 2020-11-23T08:21:43Z | - |
dc.date.issued | 2020 | - |
dc.identifier.citation | Journal of Dairy Science, 2020, v. 103 n. 8, p. 6830-6842 | - |
dc.identifier.issn | 0022-0302 | - |
dc.identifier.uri | http://hdl.handle.net/10722/293782 | - |
dc.description | Bronze open access | - |
dc.description.abstract | In this study, we purified and characterized exopolysaccharide (EPS) produced by a high-EPS-producing dairy starter bacterium, Streptococcus thermophilus ASCC 1275. Crude EPS was extracted from S. thermophilus ASCC 1275 and partially purified using dialysis. Further purification and fractionation of exopolysaccharide was conducted using HPLC on a Superose 6 column (Cytiva/Global Life Sciences Solutions, Marlborough, MA). Glycosyl composition analysis, linkage analysis along with 1-dimensional and 2-dimensional nuclear magnetic resonance spectroscopy were performed to deduce the structure of EPS. Three fractions (F) obtained from gel permeation chromatography were termed F1 (2.6%), F2 (45.8%), and F3 (51.6%) with average molecular weights of approximately 511, 40, and 5 kDa, respectively. Monosaccharide composition analysis revealed the dominance of glucose, galactose, and mannose in all 3 fractions. Major linkages observed in F3 were terminal galactopyranosyl (t-Gal), 3-linked glucopyranosyl (3-Glc), 3-linked galactofuranosyl (3-Galf), and 3,6-linked glucopyranosyl (3,6-Glc) and major linkages present in F2 were 4-Glc (48 mol%), followed by terminal mannopyranosyl (t-Man), 2- + 3-linked mannopyranosyl (2-Man+3-Man), and 2,6-linked mannopyranosyl (2,6-Man; total ∼28 mol%). The 1-dimensional and 2-dimensional nuclear magnetic resonance spectroscopy revealed that F2 comprised mannans linked by (1→2) linkages and F3 consisted of linear chains of α-d-glucopyranosyl (α-d-Glcp), β-d-glucopyranosyl (β-d-Glcp), and β-d-galactofuranosyl (β-d-Galf) connected by (1→3) linkages; branching was through (1→6) linkage in F3. A possible structure of EPS in F2 and F3 was proposed. | - |
dc.language | eng | - |
dc.publisher | Elsevier, published in association with American Dairy Science Association. The Journal's web site is located at http://www.journalofdairyscience.org/ | - |
dc.relation.ispartof | Journal of Dairy Science | - |
dc.subject | exopolysaccharide | - |
dc.subject | structure | - |
dc.subject | glycosyl linkage | - |
dc.subject | nuclear magnetic resonance (NMR) | - |
dc.title | Structural characterization of exopolysaccharide from Streptococcus thermophilus ASCC 1275 | - |
dc.type | Article | - |
dc.identifier.email | Shah, NP: npshah@hku.hk | - |
dc.identifier.authority | Shah, NP=rp01571 | - |
dc.description.nature | link_to_OA_fulltext | - |
dc.identifier.doi | 10.3168/jds.2019-17439 | - |
dc.identifier.pmid | 32475665 | - |
dc.identifier.scopus | eid_2-s2.0-85085567837 | - |
dc.identifier.hkuros | 320069 | - |
dc.identifier.volume | 103 | - |
dc.identifier.issue | 8 | - |
dc.identifier.spage | 6830 | - |
dc.identifier.epage | 6842 | - |
dc.identifier.isi | WOS:000550190500010 | - |
dc.publisher.place | United States | - |