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Article: Antigen Capture Enzyme-Linked Immunosorbent Assay for Detecting Middle East Respiratory Syndrome Coronavirus in Humans

TitleAntigen Capture Enzyme-Linked Immunosorbent Assay for Detecting Middle East Respiratory Syndrome Coronavirus in Humans
Authors
KeywordsMERS-CoV
Nucleocapsid protein
Molecular detection
Clinical diagnosis
Issue Date2020
PublisherHumana Press, Inc. The Journal's web site is located at http://link.springer.com/bookseries/7651
Citation
Methods in Molecular Biology, 2020, v. 2099, p. 89-97 How to Cite?
AbstractThe Middle East respiratory syndrome (MERS) is the second novel zoonotic disease infecting humans caused by coronavirus (CoV) in this century. To date, more than 2200 laboratory-confirmed human cases have been identified in 27 countries, and more than 800 MERS-CoV associated deaths have been reported since its outbreak in 2012. Rapid laboratory diagnosis of MERS-CoV is the key to successful containment and prevention of the spread of infection. Though the gold standard for diagnosing MERS-CoV infection in humans is still nucleic acid amplification test (NAAT) of the up-E region, an antigen capture enzyme-linked immunosorbent assay (ELISA) could also be of use for early diagnosis in less developed locations. In the present method, a step-by-step guide to perform a MERS-CoV nucleocapsid protein (NP) capture ELISA using two NP-specific monoclonal antibodies is provided for readers to develop their in-house workflow or diagnostic kit for clinical use and for mass-screening project of animals (e.g., dromedaries and bats) to better understand the spread and evolution of the virus.
DescriptionBronze open access
Persistent Identifierhttp://hdl.handle.net/10722/293688
ISSN
2023 SCImago Journal Rankings: 0.399
PubMed Central ID

 

DC FieldValueLanguage
dc.contributor.authorFung, J-
dc.contributor.authorLau, SKP-
dc.contributor.authorWoo, PCY-
dc.date.accessioned2020-11-23T08:20:23Z-
dc.date.available2020-11-23T08:20:23Z-
dc.date.issued2020-
dc.identifier.citationMethods in Molecular Biology, 2020, v. 2099, p. 89-97-
dc.identifier.issn1064-3745-
dc.identifier.urihttp://hdl.handle.net/10722/293688-
dc.descriptionBronze open access-
dc.description.abstractThe Middle East respiratory syndrome (MERS) is the second novel zoonotic disease infecting humans caused by coronavirus (CoV) in this century. To date, more than 2200 laboratory-confirmed human cases have been identified in 27 countries, and more than 800 MERS-CoV associated deaths have been reported since its outbreak in 2012. Rapid laboratory diagnosis of MERS-CoV is the key to successful containment and prevention of the spread of infection. Though the gold standard for diagnosing MERS-CoV infection in humans is still nucleic acid amplification test (NAAT) of the up-E region, an antigen capture enzyme-linked immunosorbent assay (ELISA) could also be of use for early diagnosis in less developed locations. In the present method, a step-by-step guide to perform a MERS-CoV nucleocapsid protein (NP) capture ELISA using two NP-specific monoclonal antibodies is provided for readers to develop their in-house workflow or diagnostic kit for clinical use and for mass-screening project of animals (e.g., dromedaries and bats) to better understand the spread and evolution of the virus.-
dc.languageeng-
dc.publisherHumana Press, Inc. The Journal's web site is located at http://link.springer.com/bookseries/7651-
dc.relation.ispartofMethods in Molecular Biology-
dc.rightsThe original publication is available at www.springerlink.com-
dc.subjectMERS-CoV-
dc.subjectNucleocapsid protein-
dc.subjectMolecular detection-
dc.subjectClinical diagnosis-
dc.titleAntigen Capture Enzyme-Linked Immunosorbent Assay for Detecting Middle East Respiratory Syndrome Coronavirus in Humans-
dc.typeArticle-
dc.identifier.emailLau, SKP: skplau@hkucc.hku.hk-
dc.identifier.emailWoo, PCY: pcywoo@hkucc.hku.hk-
dc.identifier.authorityLau, SKP=rp00486-
dc.identifier.authorityWoo, PCY=rp00430-
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1007/978-1-0716-0211-9_7-
dc.identifier.pmid31883089-
dc.identifier.pmcidPMC7123003-
dc.identifier.scopuseid_2-s2.0-85077340905-
dc.identifier.hkuros319727-
dc.identifier.volume2099-
dc.identifier.spage89-
dc.identifier.epage97-
dc.publisher.placeUnited States-

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