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Article: Cytogenetic of Brachyura (Decapoda): testing technical aspects for obtaining metaphase chromosomes in six mangrove crab species

TitleCytogenetic of Brachyura (Decapoda): testing technical aspects for obtaining metaphase chromosomes in six mangrove crab species
Authors
Keywordsaquaculture
chromosomes
confocal microscope
mangrove crabs
DAPI
Issue Date2020
PublisherFirenze University Press. The Journal's web site is located at https://riviste.fupress.net/index.php/caryologia/index
Citation
Caryologia, 2020, v. 73 n. 2, p. 39-49 How to Cite?
AbstractBrachyura is one of the most specious infra-order belonging to Decapoda and it plays a central role from an ecological and economic point of view. Despite its importance, cytogenetic studies on Brachyura (Decapoda) are extremely limited due to the difficulties in obtaining chromosome preparations of good quality. Molecular cytogenetic have proven to provide basic knowledge on the genome organization of species and in methods for manipulating genomes. It is also very useful to support aquaculture. In this study we focused on six semi-terrestrial mangrove crab species to test several variations of technical steps to produce chromosome preparations in Brachyura. The best results were obtained using cells from early stage embryos incubated with 0.0005% nocodazole or 0.05% colchicine and hypotonized with 0.075 M KCl solution. The best method to analyze the chromosomes was the use of confocal microscope after DAPI staining. We recorded a high chromosome number for the six study species. Similar chromosome morphology was recorded for allied species likely due to phylogenetic relationship. Variable results with cytogenetic treatments in different species suggest that there may be a species-specific response to the techniques we tested. Chromosome number ranges reported in this study will be useful in future genome sequencing studies, i.e. to assess the quality of sequencing assemblies.
Persistent Identifierhttp://hdl.handle.net/10722/293633
ISSN
2022 Impact Factor: 2.1
2023 SCImago Journal Rankings: 0.182
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorIannucci, A-
dc.contributor.authorCannicci, S-
dc.contributor.authorLin, Z-
dc.contributor.authorYuen, KWY-
dc.contributor.authorCiofi, C-
dc.contributor.authorStanyon, R-
dc.contributor.authorFratini, S-
dc.date.accessioned2020-11-23T08:19:35Z-
dc.date.available2020-11-23T08:19:35Z-
dc.date.issued2020-
dc.identifier.citationCaryologia, 2020, v. 73 n. 2, p. 39-49-
dc.identifier.issn0008-7114-
dc.identifier.urihttp://hdl.handle.net/10722/293633-
dc.description.abstractBrachyura is one of the most specious infra-order belonging to Decapoda and it plays a central role from an ecological and economic point of view. Despite its importance, cytogenetic studies on Brachyura (Decapoda) are extremely limited due to the difficulties in obtaining chromosome preparations of good quality. Molecular cytogenetic have proven to provide basic knowledge on the genome organization of species and in methods for manipulating genomes. It is also very useful to support aquaculture. In this study we focused on six semi-terrestrial mangrove crab species to test several variations of technical steps to produce chromosome preparations in Brachyura. The best results were obtained using cells from early stage embryos incubated with 0.0005% nocodazole or 0.05% colchicine and hypotonized with 0.075 M KCl solution. The best method to analyze the chromosomes was the use of confocal microscope after DAPI staining. We recorded a high chromosome number for the six study species. Similar chromosome morphology was recorded for allied species likely due to phylogenetic relationship. Variable results with cytogenetic treatments in different species suggest that there may be a species-specific response to the techniques we tested. Chromosome number ranges reported in this study will be useful in future genome sequencing studies, i.e. to assess the quality of sequencing assemblies.-
dc.languageeng-
dc.publisherFirenze University Press. The Journal's web site is located at https://riviste.fupress.net/index.php/caryologia/index-
dc.relation.ispartofCaryologia-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.subjectaquaculture-
dc.subjectchromosomes-
dc.subjectconfocal microscope-
dc.subjectmangrove crabs-
dc.subjectDAPI-
dc.titleCytogenetic of Brachyura (Decapoda): testing technical aspects for obtaining metaphase chromosomes in six mangrove crab species-
dc.typeArticle-
dc.identifier.emailCannicci, S: cannicci@hku.hk-
dc.identifier.emailLin, Z: wzylin@connect.hku.hk-
dc.identifier.emailYuen, KWY: kwyyuen@hku.hk-
dc.identifier.authorityCannicci, S=rp02079-
dc.identifier.authorityYuen, KWY=rp01512-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.13128/caryologia-791-
dc.identifier.scopuseid_2-s2.0-85089117116-
dc.identifier.hkuros320195-
dc.identifier.volume73-
dc.identifier.issue2-
dc.identifier.spage39-
dc.identifier.epage49-
dc.identifier.isiWOS:000556005200004-
dc.publisher.placeItaly-

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