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Article: Effects of supplementation of citrulline and Lactobacillus helveticus ASCC 511 on intestinal epithelial cell integrity
Title | Effects of supplementation of citrulline and Lactobacillus helveticus ASCC 511 on intestinal epithelial cell integrity |
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Authors | |
Keywords | Citrulline Lactobacillus helveticus Cell integrity Tight junction Anti-adhesion effect |
Issue Date | 2020 |
Publisher | Elsevier BV. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/717426/description#description |
Citation | Journal of Functional Foods, 2020, v. 64, p. article no. 103571 How to Cite? |
Abstract | Citrulline is a precursor of arginine and is believed to have the same beneficial effects as arginine. Lactobacillus helveticus ASCC 511 (LH511) utilizes arginine to produce extra energy for cell growth via arginine deiminase (ADI) pathway. Supplementation of both citrulline and LH511 is considered to be beneficial to intestinal tract. LH511 + Cit-2 mM was tested with IPEC-J2 cells to determine (i) the anti-adhesion effect against pathogenic infection (ii) effect on cell integrity by measuring transepithelial electrical resistance (TEER) and (iii) effect on tight junction (TJ)-proteins expression by qPCR and western-blot analyses. LH511 + Cit-2 mM exhibited a protective effect against adhesion of enterohemorrhagic (EHEC) and enteroinvasive (EIEC) Escherichia coli; it stimulated nitric oxide (NO) production, improved TEER and stimulated TJ-proteins expression. LH511 + Cit-2 mM showed better effects than that with arginine and citrulline alone. This study suggests supplementation of citrulline with LH511 has a synergistic effect and it might be a potential supplement for enhancing the health of the intestine. |
Description | Hybrid open access |
Persistent Identifier | http://hdl.handle.net/10722/293630 |
ISSN | 2023 Impact Factor: 3.8 2023 SCImago Journal Rankings: 0.900 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | HO, SW | - |
dc.contributor.author | El-Nezamy, H | - |
dc.contributor.author | Shah, NP | - |
dc.date.accessioned | 2020-11-23T08:19:33Z | - |
dc.date.available | 2020-11-23T08:19:33Z | - |
dc.date.issued | 2020 | - |
dc.identifier.citation | Journal of Functional Foods, 2020, v. 64, p. article no. 103571 | - |
dc.identifier.issn | 1756-4646 | - |
dc.identifier.uri | http://hdl.handle.net/10722/293630 | - |
dc.description | Hybrid open access | - |
dc.description.abstract | Citrulline is a precursor of arginine and is believed to have the same beneficial effects as arginine. Lactobacillus helveticus ASCC 511 (LH511) utilizes arginine to produce extra energy for cell growth via arginine deiminase (ADI) pathway. Supplementation of both citrulline and LH511 is considered to be beneficial to intestinal tract. LH511 + Cit-2 mM was tested with IPEC-J2 cells to determine (i) the anti-adhesion effect against pathogenic infection (ii) effect on cell integrity by measuring transepithelial electrical resistance (TEER) and (iii) effect on tight junction (TJ)-proteins expression by qPCR and western-blot analyses. LH511 + Cit-2 mM exhibited a protective effect against adhesion of enterohemorrhagic (EHEC) and enteroinvasive (EIEC) Escherichia coli; it stimulated nitric oxide (NO) production, improved TEER and stimulated TJ-proteins expression. LH511 + Cit-2 mM showed better effects than that with arginine and citrulline alone. This study suggests supplementation of citrulline with LH511 has a synergistic effect and it might be a potential supplement for enhancing the health of the intestine. | - |
dc.language | eng | - |
dc.publisher | Elsevier BV. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/717426/description#description | - |
dc.relation.ispartof | Journal of Functional Foods | - |
dc.rights | This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License. | - |
dc.subject | Citrulline | - |
dc.subject | Lactobacillus helveticus | - |
dc.subject | Cell integrity | - |
dc.subject | Tight junction | - |
dc.subject | Anti-adhesion effect | - |
dc.title | Effects of supplementation of citrulline and Lactobacillus helveticus ASCC 511 on intestinal epithelial cell integrity | - |
dc.type | Article | - |
dc.identifier.email | El-Nezamy, H: elnezami@hkucc.hku.hk | - |
dc.identifier.email | Shah, NP: npshah@hku.hk | - |
dc.identifier.authority | El-Nezamy, H=rp00694 | - |
dc.identifier.authority | Shah, NP=rp01571 | - |
dc.description.nature | published_or_final_version | - |
dc.identifier.doi | 10.1016/j.jff.2019.103571 | - |
dc.identifier.scopus | eid_2-s2.0-85074531627 | - |
dc.identifier.hkuros | 320133 | - |
dc.identifier.volume | 64 | - |
dc.identifier.spage | article no. 103571 | - |
dc.identifier.epage | article no. 103571 | - |
dc.identifier.isi | WOS:000508491000010 | - |
dc.publisher.place | Netherlands | - |