File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: The Prevalence of Integrons as the Carrier of Antibiotic Resistance Genes in Natural and Man-Made Environments

TitleThe Prevalence of Integrons as the Carrier of Antibiotic Resistance Genes in Natural and Man-Made Environments
Authors
KeywordsAminoglycoside resistance
Anthropogenic pollution
Antibiotic resistance genes
Class 1 integrons
Environmental sample
Issue Date2017
PublisherAmerican Chemical Society. The Journal's web site is located at http://pubs.acs.org/journal/esthag
Citation
Environmental Science & Technology, 2017, v. 51 n. 10, p. 5721-5728 How to Cite?
AbstractClass 1 integrase intI1 has been considered as a good proxy for anthropogenic pollution because of being linked to genes conferring resistance to antibiotics. The gene cassettes of class 1 integrons could carry diverse antibiotic resistance genes (ARGs) and conduct horizontal gene transfer among microorganisms. The present study applied high-throughput sequencing technique combined with an intI1 database and genome assembly to quantify the abundance of intI1 in 64 environmental samples from 8 ecosystems, and to investigate the diverse arrangements of ARG-carrying gene cassettes (ACGCs) carried by class 1 integrons. The abundance of detected intI1 ranged from 3.83 × 10–4 to 4.26 × 10° intI1/cell. High correlation (Pearson’s r = 0.852) between intI1 and ARG abundance indicated that intI1 could be considered as an important indicator of ARGs in environments. Aminoglycoside resistance genes were most frequently observed on gene cassettes, carried by 57% assembled ACGCs, followed by trimethoprim and beta-lactam resistance genes. This study established the pipeline for broad monitoring of intI1 in various environmental samples and scanning the ARGs carried by integrons. These findings supplemented our knowledge on the distribution of class 1 integrons and ARGs carried on mobile genetic elements, benefiting future studies on horizontal gene transfer of ARGs.
Persistent Identifierhttp://hdl.handle.net/10722/293563
ISSN
2023 Impact Factor: 10.8
2023 SCImago Journal Rankings: 3.516
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorMa, L-
dc.contributor.authorLi, AD-
dc.contributor.authorYin, XL-
dc.contributor.authorZhang, T-
dc.date.accessioned2020-11-23T08:18:36Z-
dc.date.available2020-11-23T08:18:36Z-
dc.date.issued2017-
dc.identifier.citationEnvironmental Science & Technology, 2017, v. 51 n. 10, p. 5721-5728-
dc.identifier.issn0013-936X-
dc.identifier.urihttp://hdl.handle.net/10722/293563-
dc.description.abstractClass 1 integrase intI1 has been considered as a good proxy for anthropogenic pollution because of being linked to genes conferring resistance to antibiotics. The gene cassettes of class 1 integrons could carry diverse antibiotic resistance genes (ARGs) and conduct horizontal gene transfer among microorganisms. The present study applied high-throughput sequencing technique combined with an intI1 database and genome assembly to quantify the abundance of intI1 in 64 environmental samples from 8 ecosystems, and to investigate the diverse arrangements of ARG-carrying gene cassettes (ACGCs) carried by class 1 integrons. The abundance of detected intI1 ranged from 3.83 × 10–4 to 4.26 × 10° intI1/cell. High correlation (Pearson’s r = 0.852) between intI1 and ARG abundance indicated that intI1 could be considered as an important indicator of ARGs in environments. Aminoglycoside resistance genes were most frequently observed on gene cassettes, carried by 57% assembled ACGCs, followed by trimethoprim and beta-lactam resistance genes. This study established the pipeline for broad monitoring of intI1 in various environmental samples and scanning the ARGs carried by integrons. These findings supplemented our knowledge on the distribution of class 1 integrons and ARGs carried on mobile genetic elements, benefiting future studies on horizontal gene transfer of ARGs.-
dc.languageeng-
dc.publisherAmerican Chemical Society. The Journal's web site is located at http://pubs.acs.org/journal/esthag-
dc.relation.ispartofEnvironmental Science & Technology-
dc.rightsThis document is the Accepted Manuscript version of a Published Work that appeared in final form in [JournalTitle], copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see [insert ACS Articles on Request author-directed link to Published Work, see http://pubs.acs.org/page/policy/articlesonrequest/index.html].-
dc.subjectAminoglycoside resistance-
dc.subjectAnthropogenic pollution-
dc.subjectAntibiotic resistance genes-
dc.subjectClass 1 integrons-
dc.subjectEnvironmental sample-
dc.titleThe Prevalence of Integrons as the Carrier of Antibiotic Resistance Genes in Natural and Man-Made Environments-
dc.typeArticle-
dc.identifier.emailZhang, T: zhangt@hkucc.hku.hk-
dc.identifier.authorityZhang, T=rp00211-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1021/acs.est.6b05887-
dc.identifier.pmid28426231-
dc.identifier.scopuseid_2-s2.0-85020931661-
dc.identifier.hkuros319378-
dc.identifier.volume51-
dc.identifier.issue10-
dc.identifier.spage5721-
dc.identifier.epage5728-
dc.identifier.isiWOS:000401674400041-
dc.publisher.placeUnited States-
dc.identifier.issnl0013-936X-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats