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Article: MiR-497-5p Regulates Osteo/Odontogenic Differentiation of Stem Cells From Apical Papilla via the Smad Signaling Pathway by Targeting Smurf2
Title | MiR-497-5p Regulates Osteo/Odontogenic Differentiation of Stem Cells From Apical Papilla via the Smad Signaling Pathway by Targeting Smurf2 |
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Authors | |
Keywords | miR-497-5p stem cells from apical papilla Smurf2 Smad signaling pathway osteo/odontogenic differentiation |
Issue Date | 2020 |
Publisher | Frontiers Research Foundation. The Journal's web site is located at http://www.frontiersin.org/genetics |
Citation | Frontiers in Genetics, 2020, v. 11, p. article no. 582366 How to Cite? |
Abstract | Osteo/odontogenic differentiation is a key process of human stem cells from apical papilla (SCAP) in tooth root development. Emerging evidence indicates microRNAs (miRNAs) play diverse roles in osteogenesis. However, their functions in osteo/odontogenic differentiation of SCAP require further elucidation. To investigate the role of miRNA in SCAP osteo/odontogenic differentiation and underlying mechanisms, miRNA microarray analysis was performed to screen differentially expressed miRNAs between control and osteo/odontogenic-induced group. Quantitative real-time PCR (qRT-PCR) and western blot were used to detected osteo/odontogenic differentiation-related markers and possible signaling pathway SCAP-associated genes. Alizarin Red Staining (ARS) were applied to evaluated osteogenic capacity. The results showed that miR-497-5p increased during SCAP osteo/odontogenic differentiation. Overexpression of miR-497-5p enhanced the osteo/odontogenic differentiation of SCAP, whereas downregulation of miR-497-5p elicited the opposite effect, thus suggesting that miR-497-5p is a positive regulator of the osteo/odontogenic differentiation of SCAP. Bioinformatic analysis and dual luciferase reporter assay identified that SMAD specific E3 ubiquitin protein ligase 2 (Smurf2) is a direct target of miR-497-5p. Further study demonstrated that Smurf2 negatively regulates SCAP osteo/odontogenic differentiation, and silencing Smurf2 could block the inhibitory effect of the miR-497-5p inhibitor. Meanwhile, pathway detection manifested that miR-497-5p promotes osteo/odontogenic differentiation via Smad signaling pathway. Collectively, our findings demostrate that miR-497-5p promotes osteo/odontogenic differentiation of SCAP via Smad signaling pathway by targeting Smurf2. © Copyright © 2020 Liu, Wang, Song, Du, Yu, Zheng, Zhang and Wang. |
Persistent Identifier | http://hdl.handle.net/10722/293516 |
ISSN | 2021 Impact Factor: 4.772 2020 SCImago Journal Rankings: 1.413 |
PubMed Central ID | |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | LIU, J | - |
dc.contributor.author | Wang, X | - |
dc.contributor.author | Song, M | - |
dc.contributor.author | Du, J | - |
dc.contributor.author | Yu, J | - |
dc.contributor.author | Zheng, W | - |
dc.contributor.author | Zhang, C | - |
dc.contributor.author | Wang, Y | - |
dc.date.accessioned | 2020-11-23T08:17:54Z | - |
dc.date.available | 2020-11-23T08:17:54Z | - |
dc.date.issued | 2020 | - |
dc.identifier.citation | Frontiers in Genetics, 2020, v. 11, p. article no. 582366 | - |
dc.identifier.issn | 1664-8021 | - |
dc.identifier.uri | http://hdl.handle.net/10722/293516 | - |
dc.description.abstract | Osteo/odontogenic differentiation is a key process of human stem cells from apical papilla (SCAP) in tooth root development. Emerging evidence indicates microRNAs (miRNAs) play diverse roles in osteogenesis. However, their functions in osteo/odontogenic differentiation of SCAP require further elucidation. To investigate the role of miRNA in SCAP osteo/odontogenic differentiation and underlying mechanisms, miRNA microarray analysis was performed to screen differentially expressed miRNAs between control and osteo/odontogenic-induced group. Quantitative real-time PCR (qRT-PCR) and western blot were used to detected osteo/odontogenic differentiation-related markers and possible signaling pathway SCAP-associated genes. Alizarin Red Staining (ARS) were applied to evaluated osteogenic capacity. The results showed that miR-497-5p increased during SCAP osteo/odontogenic differentiation. Overexpression of miR-497-5p enhanced the osteo/odontogenic differentiation of SCAP, whereas downregulation of miR-497-5p elicited the opposite effect, thus suggesting that miR-497-5p is a positive regulator of the osteo/odontogenic differentiation of SCAP. Bioinformatic analysis and dual luciferase reporter assay identified that SMAD specific E3 ubiquitin protein ligase 2 (Smurf2) is a direct target of miR-497-5p. Further study demonstrated that Smurf2 negatively regulates SCAP osteo/odontogenic differentiation, and silencing Smurf2 could block the inhibitory effect of the miR-497-5p inhibitor. Meanwhile, pathway detection manifested that miR-497-5p promotes osteo/odontogenic differentiation via Smad signaling pathway. Collectively, our findings demostrate that miR-497-5p promotes osteo/odontogenic differentiation of SCAP via Smad signaling pathway by targeting Smurf2. © Copyright © 2020 Liu, Wang, Song, Du, Yu, Zheng, Zhang and Wang. | - |
dc.language | eng | - |
dc.publisher | Frontiers Research Foundation. The Journal's web site is located at http://www.frontiersin.org/genetics | - |
dc.relation.ispartof | Frontiers in Genetics | - |
dc.rights | This Document is Protected by copyright and was first published by Frontiers. All rights reserved. It is reproduced with permission. | - |
dc.rights | This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License. | - |
dc.subject | miR-497-5p | - |
dc.subject | stem cells from apical papilla | - |
dc.subject | Smurf2 | - |
dc.subject | Smad signaling pathway | - |
dc.subject | osteo/odontogenic differentiation | - |
dc.title | MiR-497-5p Regulates Osteo/Odontogenic Differentiation of Stem Cells From Apical Papilla via the Smad Signaling Pathway by Targeting Smurf2 | - |
dc.type | Article | - |
dc.identifier.email | Zhang, C: zhangcf@hku.hk | - |
dc.identifier.authority | Zhang, C=rp01408 | - |
dc.description.nature | published_or_final_version | - |
dc.identifier.doi | 10.3389/fgene.2020.582366 | - |
dc.identifier.pmid | 33193708 | - |
dc.identifier.pmcid | PMC7662069 | - |
dc.identifier.scopus | eid_2-s2.0-85096013495 | - |
dc.identifier.hkuros | 318812 | - |
dc.identifier.volume | 11 | - |
dc.identifier.spage | article no. 582366 | - |
dc.identifier.epage | article no. 582366 | - |
dc.identifier.isi | WOS:000588785700001 | - |
dc.publisher.place | Switzerland | - |
dc.identifier.issnl | 1664-8021 | - |