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Article: Cultivation-dependent and high-throughput sequencing approaches studying the co-occurrence of antibiotic resistance genes in municipal sewage system

TitleCultivation-dependent and high-throughput sequencing approaches studying the co-occurrence of antibiotic resistance genes in municipal sewage system
Authors
KeywordsAntibiotic resistance
Municipal sewage
Coliform
High-throughput sequencing
Functional screening
Issue Date2017
PublisherSpringer. The Journal's web site is located at http://link.springer.de/link/service/journals/00253/index.htm
Citation
Applied Microbiology and Biotechnology, 2017, v. 101 n. 22, p. 8197-8207 How to Cite?
AbstractDuring the past years, antibiotic-resistant bacteria (ARB) leading for the spreading of antibiotic resistance genes (ARGs) became a global problem, especially multidrug-resistant (MDR) bacteria are considered the prime culprit of antibiotic resistance. However, the correlation between the antibiotic-resistant phenotype and the ARG profiles remains poorly understood. In the present study, metagenomic functional screening and metagenomic analysis of coliforms were combined to explore the phenotype and genotype of the ARBs from municipal sewage. Our results showed that the ARG co-occurrence was widespread in the municipal sewage. The present study also highlighted the high abundance of ARGs from antibiotic resistance coliforms especially the MDR coliforms with ARG level of 33.8 ± 4.2 copies per cell. The ARG profiles and the antibiotic resistance phenotypes of the isolated antibiotic resistant coliforms were also correlated and indicated that the resistance to the related antibiotic (ampicillin, kanamycin, erythromycin, chloramphenicol, and tetracycline) was mostly contributed by the ARGs belonging to the subtypes of β-lactamase, aminoglycoside 3-phosphotransferase, phosphotransferase type 2, chloramphenicol acetyltransferase, tetA, etc.
Persistent Identifierhttp://hdl.handle.net/10722/293299
ISSN
2019 Impact Factor: 3.53
2015 SCImago Journal Rankings: 1.262
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorLi, AD-
dc.contributor.authorMa, L-
dc.contributor.authorJiang, XT-
dc.contributor.authorZhang, T-
dc.date.accessioned2020-11-23T08:14:45Z-
dc.date.available2020-11-23T08:14:45Z-
dc.date.issued2017-
dc.identifier.citationApplied Microbiology and Biotechnology, 2017, v. 101 n. 22, p. 8197-8207-
dc.identifier.issn0175-7598-
dc.identifier.urihttp://hdl.handle.net/10722/293299-
dc.description.abstractDuring the past years, antibiotic-resistant bacteria (ARB) leading for the spreading of antibiotic resistance genes (ARGs) became a global problem, especially multidrug-resistant (MDR) bacteria are considered the prime culprit of antibiotic resistance. However, the correlation between the antibiotic-resistant phenotype and the ARG profiles remains poorly understood. In the present study, metagenomic functional screening and metagenomic analysis of coliforms were combined to explore the phenotype and genotype of the ARBs from municipal sewage. Our results showed that the ARG co-occurrence was widespread in the municipal sewage. The present study also highlighted the high abundance of ARGs from antibiotic resistance coliforms especially the MDR coliforms with ARG level of 33.8 ± 4.2 copies per cell. The ARG profiles and the antibiotic resistance phenotypes of the isolated antibiotic resistant coliforms were also correlated and indicated that the resistance to the related antibiotic (ampicillin, kanamycin, erythromycin, chloramphenicol, and tetracycline) was mostly contributed by the ARGs belonging to the subtypes of β-lactamase, aminoglycoside 3-phosphotransferase, phosphotransferase type 2, chloramphenicol acetyltransferase, tetA, etc.-
dc.languageeng-
dc.publisherSpringer. The Journal's web site is located at http://link.springer.de/link/service/journals/00253/index.htm-
dc.relation.ispartofApplied Microbiology and Biotechnology-
dc.rightsThis is a post-peer-review, pre-copyedit version of an article published in [insert journal title]. The final authenticated version is available online at: https://doi.org/[insert DOI]-
dc.subjectAntibiotic resistance-
dc.subjectMunicipal sewage-
dc.subjectColiform-
dc.subjectHigh-throughput sequencing-
dc.subjectFunctional screening-
dc.titleCultivation-dependent and high-throughput sequencing approaches studying the co-occurrence of antibiotic resistance genes in municipal sewage system-
dc.typeArticle-
dc.identifier.emailZhang, T: zhangt@hkucc.hku.hk-
dc.identifier.authorityZhang, T=rp00211-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1007/s00253-017-8573-1-
dc.identifier.pmid29034431-
dc.identifier.scopuseid_2-s2.0-85031496602-
dc.identifier.hkuros319392-
dc.identifier.volume101-
dc.identifier.issue22-
dc.identifier.spage8197-
dc.identifier.epage8207-
dc.identifier.isiWOS:000413685300011-
dc.publisher.placeGermany-
dc.identifier.issnl0175-7598-

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