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Article: Kilohertz two-photon fluorescence microscopy imaging of neural activity in vivo

TitleKilohertz two-photon fluorescence microscopy imaging of neural activity in vivo
Authors
Issue Date2020
PublisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/nmeth
Citation
Nature Methods, 2020, v. 17 n. 3, p. 287-290 How to Cite?
AbstractUnderstanding information processing in the brain requires monitoring neuronal activity at high spatiotemporal resolution. Using an ultrafast two-photon fluorescence microscope empowered by all-optical laser scanning, we imaged neuronal activity in vivo at up to 3,000 frames per second and submicrometer spatial resolution. This imaging method enabled monitoring of both supra- and subthreshold electrical activity down to 345 μm below the brain surface in head-fixed awake mice.
Persistent Identifierhttp://hdl.handle.net/10722/293154
ISSN
2023 Impact Factor: 36.1
2023 SCImago Journal Rankings: 14.796
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorWu, J-
dc.contributor.authorLiang, Y-
dc.contributor.authorChen, S-
dc.contributor.authorHsu, CL-
dc.contributor.authorChavarha, M-
dc.contributor.authorEvans, SW-
dc.contributor.authorShi, D-
dc.contributor.authorLin, MZ-
dc.contributor.authorTsia, KK-
dc.contributor.authorJi, N-
dc.date.accessioned2020-11-23T08:12:36Z-
dc.date.available2020-11-23T08:12:36Z-
dc.date.issued2020-
dc.identifier.citationNature Methods, 2020, v. 17 n. 3, p. 287-290-
dc.identifier.issn1548-7091-
dc.identifier.urihttp://hdl.handle.net/10722/293154-
dc.description.abstractUnderstanding information processing in the brain requires monitoring neuronal activity at high spatiotemporal resolution. Using an ultrafast two-photon fluorescence microscope empowered by all-optical laser scanning, we imaged neuronal activity in vivo at up to 3,000 frames per second and submicrometer spatial resolution. This imaging method enabled monitoring of both supra- and subthreshold electrical activity down to 345 μm below the brain surface in head-fixed awake mice.-
dc.languageeng-
dc.publisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/nmeth-
dc.relation.ispartofNature Methods-
dc.titleKilohertz two-photon fluorescence microscopy imaging of neural activity in vivo-
dc.typeArticle-
dc.identifier.emailTsia, KK: tsia@hku.hk-
dc.identifier.authorityTsia, KK=rp01389-
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1038/s41592-020-0762-7-
dc.identifier.pmid32123392-
dc.identifier.pmcidPMC7199528-
dc.identifier.scopuseid_2-s2.0-85081080377-
dc.identifier.hkuros318979-
dc.identifier.volume17-
dc.identifier.issue3-
dc.identifier.spage287-
dc.identifier.epage290-
dc.identifier.isiWOS:000517743000007-
dc.publisher.placeUnited Kingdom-
dc.identifier.issnl1548-7091-

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