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- Publisher Website: 10.1002/jcp.25516
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- PMID: 27509531
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Article: DJ-1/Park7 Sensitive Na+/H+ Exchanger 1 (NHE1) in CD4+ T Cells
Title | DJ-1/Park7 Sensitive Na<sup>+</sup>/H<sup>+</sup> Exchanger 1 (NHE1) in CD4<sup>+</sup> T Cells |
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Authors | |
Issue Date | 2017 |
Citation | Journal of Cellular Physiology, 2017, v. 232, n. 11, p. 3050-3059 How to Cite? |
Abstract | © 2016 Wiley Periodicals, Inc. DJ-1/Park7 is a redox-sensitive chaperone protein counteracting oxidation and presumably contributing to the control of oxidative stress responses and thus inflammation. DJ-1 gene deletion exacerbates the progression of Parkinson's disease presumably by augmenting oxidative stress. Formation of reactive oxygen species (ROS) is paralleled by activation of the Na+/H+ exchanger 1 (NHE1). ROS formation in CD4+ T cells plays a decisive role in regulating inflammatory responses. In the present study, we explored whether DJ-1 is expressed in CD4+ T cells, and affects ROS production as well as NHE1 in those cells. To this end, DJ-1 and NHE1 transcript, and protein levels were quantified by qRT-PCR and Western blotting, respectively, intracellular pH (pHi) utilizing bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein (BCECF) fluorescence, NHE activity from realkalinization after an ammonium pulse, and ROS production utilizing 2′,7′ −dichlorofluorescin diacetate (DCFDA) fluorescence. As a result DJ-1 was expressed in CD4+ T cells. ROS formation, NHE1 transcript levels, NHE1 protein, and NHE activity were higher in CD4+ T cells from DJ-1 deficient mice than in CD4+ T cells from wild type mice. Antioxidant N-acetyl-cysteine (NAC) and protein tyrosine kinase (PTK) inhibitor staurosporine decreased the NHE activity in DJ-1 deficient CD4+ T cells, and blunted the difference between DJ-1−/− and DJ-1+/+ CD4+ T cells, an observation pointing to a role of ROS in the up-regulation of NHE1 in DJ-1−/− CD4+ T cells. In conclusion, DJ-1 is a powerful regulator of ROS production as well as NHE1 expression and activity in CD4+ T cells. J. Cell. Physiol. 232: 3050–3059, 2017. © 2016 Wiley Periodicals, Inc. |
Persistent Identifier | http://hdl.handle.net/10722/293028 |
ISSN | 2023 Impact Factor: 4.5 2023 SCImago Journal Rankings: 1.321 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Zhou, Yuetao | - |
dc.contributor.author | Shi, Xiaolong | - |
dc.contributor.author | Chen, Hong | - |
dc.contributor.author | Zhang, Shaqiu | - |
dc.contributor.author | Salker, Madhuri S. | - |
dc.contributor.author | Mack, Andreas F. | - |
dc.contributor.author | Föller, Michael | - |
dc.contributor.author | Mak, Tak W. | - |
dc.contributor.author | Singh, Yogesh | - |
dc.contributor.author | Lang, Florian | - |
dc.date.accessioned | 2020-11-17T14:57:43Z | - |
dc.date.available | 2020-11-17T14:57:43Z | - |
dc.date.issued | 2017 | - |
dc.identifier.citation | Journal of Cellular Physiology, 2017, v. 232, n. 11, p. 3050-3059 | - |
dc.identifier.issn | 0021-9541 | - |
dc.identifier.uri | http://hdl.handle.net/10722/293028 | - |
dc.description.abstract | © 2016 Wiley Periodicals, Inc. DJ-1/Park7 is a redox-sensitive chaperone protein counteracting oxidation and presumably contributing to the control of oxidative stress responses and thus inflammation. DJ-1 gene deletion exacerbates the progression of Parkinson's disease presumably by augmenting oxidative stress. Formation of reactive oxygen species (ROS) is paralleled by activation of the Na+/H+ exchanger 1 (NHE1). ROS formation in CD4+ T cells plays a decisive role in regulating inflammatory responses. In the present study, we explored whether DJ-1 is expressed in CD4+ T cells, and affects ROS production as well as NHE1 in those cells. To this end, DJ-1 and NHE1 transcript, and protein levels were quantified by qRT-PCR and Western blotting, respectively, intracellular pH (pHi) utilizing bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein (BCECF) fluorescence, NHE activity from realkalinization after an ammonium pulse, and ROS production utilizing 2′,7′ −dichlorofluorescin diacetate (DCFDA) fluorescence. As a result DJ-1 was expressed in CD4+ T cells. ROS formation, NHE1 transcript levels, NHE1 protein, and NHE activity were higher in CD4+ T cells from DJ-1 deficient mice than in CD4+ T cells from wild type mice. Antioxidant N-acetyl-cysteine (NAC) and protein tyrosine kinase (PTK) inhibitor staurosporine decreased the NHE activity in DJ-1 deficient CD4+ T cells, and blunted the difference between DJ-1−/− and DJ-1+/+ CD4+ T cells, an observation pointing to a role of ROS in the up-regulation of NHE1 in DJ-1−/− CD4+ T cells. In conclusion, DJ-1 is a powerful regulator of ROS production as well as NHE1 expression and activity in CD4+ T cells. J. Cell. Physiol. 232: 3050–3059, 2017. © 2016 Wiley Periodicals, Inc. | - |
dc.language | eng | - |
dc.relation.ispartof | Journal of Cellular Physiology | - |
dc.title | DJ-1/Park7 Sensitive Na<sup>+</sup>/H<sup>+</sup> Exchanger 1 (NHE1) in CD4<sup>+</sup> T Cells | - |
dc.type | Article | - |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1002/jcp.25516 | - |
dc.identifier.pmid | 27509531 | - |
dc.identifier.scopus | eid_2-s2.0-85026297565 | - |
dc.identifier.volume | 232 | - |
dc.identifier.issue | 11 | - |
dc.identifier.spage | 3050 | - |
dc.identifier.epage | 3059 | - |
dc.identifier.eissn | 1097-4652 | - |
dc.identifier.isi | WOS:000407020800015 | - |
dc.identifier.issnl | 0021-9541 | - |