Sixty-two new microsatellite markers for an endemic frog Indirana beddomii from the Western Ghats biodiversity hotspot

Abstract

Frogs in the genus Indirana are endemic to Western Ghats biodiversity hotspot. The species are poorly studied and in many cases threatened or endangered. Here we describe primers and polymerase chain reactions for 62 microsatellite loci for Indirana beddomii, one of the commonest frogs in the genus. Fifty-six of the primers were polymorphic on sample of 23 individuals from a single sampling site (Ponmudi, Kerala) with an average 9.11 alleles per locus (range = 2-20). The average observed and expected heterozygosities were 0.64 and 0.71, respectively. The loci should be useful in conservation genetic studies of Indirana frogs. © 2010 Springer Science+Business Media B.V.