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Article: RAMHA: A PC-based Monte-Carlo simulation of random saturation mutagenesis

TitleRAMHA: A PC-based Monte-Carlo simulation of random saturation mutagenesis
Authors
KeywordsDNA systhesis
Monte Carlo simulation
Modeling
Computer program
Anti-termination strategies
Random saturation mutagenesis
HIV-1 Tat
Issue Date1993
Citation
Computers in Biology and Medicine, 1993, v. 23, n. 6, p. 463-474 How to Cite?
AbstractRandom mutagenesis is a powerful tool in protein structure-function analyses. One approach to random mutagenesis is the de novo synthesis of polypeptide-encoding oligodeoxy-nucleotides using doped nucleoside phosphoramidites. A Turbo PASCAL program, RAMHA, is described for modeling such mutagenesis. Upon entering the target sequence and the desired level of nucleotide contamination, RAMHA performs a Monte Carlo simulation of the mutagenesis, compiling statistics on the similarity of resultant mutant polypeptides to the wild-type sequence, the frequency of premature open-reading frame terminations, and other relevant outcomes. Simulated mutagenesis of two DNA targets has led to the development of two different strategies to avoid the random introduction of stop codons within mutagenized gene segments. © 1993.
Persistent Identifierhttp://hdl.handle.net/10722/292421
ISSN
2023 Impact Factor: 7.0
2023 SCImago Journal Rankings: 1.481
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorSiderovski, David P.-
dc.contributor.authorMak, Tak W.-
dc.date.accessioned2020-11-17T14:56:27Z-
dc.date.available2020-11-17T14:56:27Z-
dc.date.issued1993-
dc.identifier.citationComputers in Biology and Medicine, 1993, v. 23, n. 6, p. 463-474-
dc.identifier.issn0010-4825-
dc.identifier.urihttp://hdl.handle.net/10722/292421-
dc.description.abstractRandom mutagenesis is a powerful tool in protein structure-function analyses. One approach to random mutagenesis is the de novo synthesis of polypeptide-encoding oligodeoxy-nucleotides using doped nucleoside phosphoramidites. A Turbo PASCAL program, RAMHA, is described for modeling such mutagenesis. Upon entering the target sequence and the desired level of nucleotide contamination, RAMHA performs a Monte Carlo simulation of the mutagenesis, compiling statistics on the similarity of resultant mutant polypeptides to the wild-type sequence, the frequency of premature open-reading frame terminations, and other relevant outcomes. Simulated mutagenesis of two DNA targets has led to the development of two different strategies to avoid the random introduction of stop codons within mutagenized gene segments. © 1993.-
dc.languageeng-
dc.relation.ispartofComputers in Biology and Medicine-
dc.subjectDNA systhesis-
dc.subjectMonte Carlo simulation-
dc.subjectModeling-
dc.subjectComputer program-
dc.subjectAnti-termination strategies-
dc.subjectRandom saturation mutagenesis-
dc.subjectHIV-1 Tat-
dc.titleRAMHA: A PC-based Monte-Carlo simulation of random saturation mutagenesis-
dc.typeArticle-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/0010-4825(93)90094-H-
dc.identifier.pmid8306625-
dc.identifier.scopuseid_2-s2.0-0027495561-
dc.identifier.volume23-
dc.identifier.issue6-
dc.identifier.spage463-
dc.identifier.epage474-
dc.identifier.isiWOS:A1993MM32200005-
dc.identifier.issnl0010-4825-

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