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Article: Specific deletion of the J‐Cδ locus in murine α/β T cell clones and studies using transgenic mice

TitleSpecific deletion of the J‐C<inf>δ</inf> locus in murine α/β T cell clones and studies using transgenic mice
Authors
Issue Date1990
Citation
European Journal of Immunology, 1990, v. 20, n. 3, p. 517-522 How to Cite?
AbstractA deletion event in the T cell receptor (TcR) δ locus has been characterized in a panel of mouse α/β cytotoxic T lymphocyte (CTL) clones. Data presented here shows that Jδ1, Jδ2 and Cδ are absent from functional CTL clones while a germ‐line Dδ1 fragment is retained, thus suggesting a specific deletion of this region. We have investigated the possible significance of the J‐Cδ deletion by generating T cell lines from TcR α/β transgenic mice. Unlike control T cell lines which included a T cell line derived from a β transgenic mouse, the lines expressing the transgenic α/β heterodimer have not deleted the Cδ region. This strongly suggests that the J‐Cδ deletion event is not responsible for directing T cells to the α/β lineage, but rather is involved in the rearrangement or transcriptional activity of the α locus. In addition, to ensure that the α/β transgene does not have any inhibitory affects on the rearrangement of the δ loci in general, the γ/δ expressing dendritic epithelial Tcell (DETC) population was examined in TcR α/β transgenic mice and alterations in this T cell subset were not found. This finding that normal γ/δ DETC cells are present in α/β transgenic mice, together with the data showing that the Dδ1 region remains in an unrearranged germ‐line configuration in functional α/β CTL, suggests that commitment to the α/β or γ/δ lineage is predetermined at a particular stage in early T cell ontogeny. Copyright © 1990 Wiley‐VCH Verlag GmbH & Co. KGaA, Weinheim
Persistent Identifierhttp://hdl.handle.net/10722/292373
ISSN
2023 Impact Factor: 4.5
2023 SCImago Journal Rankings: 1.627
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorOhashi, Pamela S.-
dc.contributor.authorWallace, Valerie A.-
dc.contributor.authorBroughton, Heather-
dc.contributor.authorOhashi, Cara T.-
dc.contributor.authorFerrick, David A.-
dc.contributor.authorJost, Veronica-
dc.contributor.authorMak, Tak W.-
dc.contributor.authorHengartner, Hans-
dc.contributor.authorPircher, Hanspeter-
dc.date.accessioned2020-11-17T14:56:19Z-
dc.date.available2020-11-17T14:56:19Z-
dc.date.issued1990-
dc.identifier.citationEuropean Journal of Immunology, 1990, v. 20, n. 3, p. 517-522-
dc.identifier.issn0014-2980-
dc.identifier.urihttp://hdl.handle.net/10722/292373-
dc.description.abstractA deletion event in the T cell receptor (TcR) δ locus has been characterized in a panel of mouse α/β cytotoxic T lymphocyte (CTL) clones. Data presented here shows that Jδ1, Jδ2 and Cδ are absent from functional CTL clones while a germ‐line Dδ1 fragment is retained, thus suggesting a specific deletion of this region. We have investigated the possible significance of the J‐Cδ deletion by generating T cell lines from TcR α/β transgenic mice. Unlike control T cell lines which included a T cell line derived from a β transgenic mouse, the lines expressing the transgenic α/β heterodimer have not deleted the Cδ region. This strongly suggests that the J‐Cδ deletion event is not responsible for directing T cells to the α/β lineage, but rather is involved in the rearrangement or transcriptional activity of the α locus. In addition, to ensure that the α/β transgene does not have any inhibitory affects on the rearrangement of the δ loci in general, the γ/δ expressing dendritic epithelial Tcell (DETC) population was examined in TcR α/β transgenic mice and alterations in this T cell subset were not found. This finding that normal γ/δ DETC cells are present in α/β transgenic mice, together with the data showing that the Dδ1 region remains in an unrearranged germ‐line configuration in functional α/β CTL, suggests that commitment to the α/β or γ/δ lineage is predetermined at a particular stage in early T cell ontogeny. Copyright © 1990 Wiley‐VCH Verlag GmbH & Co. KGaA, Weinheim-
dc.languageeng-
dc.relation.ispartofEuropean Journal of Immunology-
dc.titleSpecific deletion of the J‐C<inf>δ</inf> locus in murine α/β T cell clones and studies using transgenic mice-
dc.typeArticle-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1002/eji.1830200309-
dc.identifier.pmid2318248-
dc.identifier.scopuseid_2-s2.0-0025361326-
dc.identifier.volume20-
dc.identifier.issue3-
dc.identifier.spage517-
dc.identifier.epage522-
dc.identifier.eissn1521-4141-
dc.identifier.isiWOS:A1990CY84300008-
dc.identifier.issnl0014-2980-

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