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Article: Induction of differentiation in human T-lymphoblastic leukemia cell lines by 12-O-tetradecanoylphorbol 13-acetate (TPA): Studies with monoclonal antibodies to T cells

TitleInduction of differentiation in human T-lymphoblastic leukemia cell lines by 12-O-tetradecanoylphorbol 13-acetate (TPA): Studies with monoclonal antibodies to T cells
Authors
Issue Date1982
Citation
Cellular Immunology, 1982, v. 71, n. 2, p. 396-403 How to Cite?
AbstractThe induction of differentiation in human malignant T-lymphoblastic cell lines MOLT-3 and Jurkat by the tumor promoter, 12-O-tetradecanoylphorbol 13-acetate (TPA) was examined using the monoclonal antibodies OKT3, OKT4, OKT6, and OKT8 which are known to react with human T-cell differentiation antigens. It was found that in the presence of nanomolar concentrations of TPA the proportion of OKT3+ (mature T-cell marker) cells increased while the proportion of OKT4+, OKT6+, and OKT8+ (relatively immature T-cell markers) cells decreased. These changes in the distribution of the OKT antigens in MOLT-3 cells were found to be more prominent with MOLT-3 cells than when the Jurkat cells were used. In studies using a double labeling approach it was found that although the OKT3+ and E-rosette-positive (E+) cells appeared to belong to the same subpopulations of MOLT-3 cells, the OKT3 antigen was probably not related to the receptor for sheep erythrocytes because adsorption of the OKT3 antibody did not block E-rosette formation. Studies using the DNA synthesis inhibitor, arabinosylcytidine (ara-C) also indicate that DNA synthesis was not required for the induction of more mature T-cell antigens in the malignant T-cell lines by TPA. These studies, taken together with our earlier reports, support the conclusion that namomolar concentrations of TPA can induce differentiation in these malignant T-cell lines. Furthermore we have shown that the T-cell hybridoma antibodies are useful markers to detect differentiation changes in human T cells. © 1982.
Persistent Identifierhttp://hdl.handle.net/10722/292268
ISSN
2023 Impact Factor: 3.7
2023 SCImago Journal Rankings: 1.011
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorNagasawa, Kohei-
dc.contributor.authorMak, Tak W.-
dc.date.accessioned2020-11-17T14:56:07Z-
dc.date.available2020-11-17T14:56:07Z-
dc.date.issued1982-
dc.identifier.citationCellular Immunology, 1982, v. 71, n. 2, p. 396-403-
dc.identifier.issn0008-8749-
dc.identifier.urihttp://hdl.handle.net/10722/292268-
dc.description.abstractThe induction of differentiation in human malignant T-lymphoblastic cell lines MOLT-3 and Jurkat by the tumor promoter, 12-O-tetradecanoylphorbol 13-acetate (TPA) was examined using the monoclonal antibodies OKT3, OKT4, OKT6, and OKT8 which are known to react with human T-cell differentiation antigens. It was found that in the presence of nanomolar concentrations of TPA the proportion of OKT3+ (mature T-cell marker) cells increased while the proportion of OKT4+, OKT6+, and OKT8+ (relatively immature T-cell markers) cells decreased. These changes in the distribution of the OKT antigens in MOLT-3 cells were found to be more prominent with MOLT-3 cells than when the Jurkat cells were used. In studies using a double labeling approach it was found that although the OKT3+ and E-rosette-positive (E+) cells appeared to belong to the same subpopulations of MOLT-3 cells, the OKT3 antigen was probably not related to the receptor for sheep erythrocytes because adsorption of the OKT3 antibody did not block E-rosette formation. Studies using the DNA synthesis inhibitor, arabinosylcytidine (ara-C) also indicate that DNA synthesis was not required for the induction of more mature T-cell antigens in the malignant T-cell lines by TPA. These studies, taken together with our earlier reports, support the conclusion that namomolar concentrations of TPA can induce differentiation in these malignant T-cell lines. Furthermore we have shown that the T-cell hybridoma antibodies are useful markers to detect differentiation changes in human T cells. © 1982.-
dc.languageeng-
dc.relation.ispartofCellular Immunology-
dc.titleInduction of differentiation in human T-lymphoblastic leukemia cell lines by 12-O-tetradecanoylphorbol 13-acetate (TPA): Studies with monoclonal antibodies to T cells-
dc.typeArticle-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/0008-8749(82)90273-8-
dc.identifier.pmid6982771-
dc.identifier.scopuseid_2-s2.0-0019977443-
dc.identifier.volume71-
dc.identifier.issue2-
dc.identifier.spage396-
dc.identifier.epage403-
dc.identifier.eissn1090-2163-
dc.identifier.isiWOS:A1982PJ73100017-
dc.identifier.issnl0008-8749-

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