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- Publisher Website: 10.1128/MCB.1.8.721
- Scopus: eid_2-s2.0-0019793666
- PMID: 9279385
- WOS: WOS:A1981MA40900006
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Article: Clonal analysis of the late stages of erythroleukemia induced by two distinct strains of Friend leukemia virus
Title | Clonal analysis of the late stages of erythroleukemia induced by two distinct strains of Friend leukemia virus |
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Authors | |
Issue Date | 1981 |
Citation | Molecular and Cellular Biology, 1981, v. 1, n. 8, p. 721-730 How to Cite? |
Abstract | We observed striking differences between the tumorigenic colony-forming cells present in the spleens of mice later after infection with the anemia-inducing strain of Friend leukemia virus (strain FV-A) and those present after infection with the polycythemia-inducing strain (strain FV-P). Cells within primary colonies derived from FV-A- and FV-P-transformed cells (CFU-FV-A and CFU-FV-P, respectively) contained hemoglobin and spectrin, indicating that the CFU-FV-A and CFU-FV-P were transformed erythroid progenitor cells. The proportion of cells containing hemoglobin was relatively high (>25%) in newly isolated cells lines derived from CFU-FV-P colonies, whereas cell lines derived from CFU-FV-A colonies had only low levels (0 to 2%) of hemoglobin-containing cells. A high proportion of the cell lines derived from CFU-FV-A colonies responded to pure erythropoietin and accumulated spectrin and hemoglobin, whereas the cell lines derived from CFU-FV-P colonies did not. A cytogenetic analysis indicated that primary CFU-FV-P colony cells were diploid, whereas chromosomal aberrations were observed in the immediate progeny of CFU-FV-A. The presence of unique chromosomal markers in the majority of the cells within individual colonies derived from CFU-FV-A suggested that these colonies originated from single cells. Finally, leukemic progenitor cells transformed by strain FV-A appeared to have an extensive capacity to self-renew (i.e., form secondary colonies in methylcellulose), whereas a significant proportion of the corresponding cells transformed by strain FV-P did not. In addition, the self-renewal capacity of both CFU-FV-A and CFU-FV-P increased as the disease progressed. From these observations, we propose a model for the multistage nature of Friend disease; this model involves clonal evolution and expansion from a differentiating population with limited proliferative capacity to a population with a high capacity for self-renewal and proliferation. |
Persistent Identifier | http://hdl.handle.net/10722/292262 |
ISSN | 2023 Impact Factor: 3.2 2023 SCImago Journal Rankings: 1.452 |
PubMed Central ID | |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Mager, D. | - |
dc.contributor.author | MacDonald, M. E. | - |
dc.contributor.author | Robson, I. B. | - |
dc.contributor.author | Mak, T. W. | - |
dc.contributor.author | Bernstein, A. | - |
dc.date.accessioned | 2020-11-17T14:56:06Z | - |
dc.date.available | 2020-11-17T14:56:06Z | - |
dc.date.issued | 1981 | - |
dc.identifier.citation | Molecular and Cellular Biology, 1981, v. 1, n. 8, p. 721-730 | - |
dc.identifier.issn | 0270-7306 | - |
dc.identifier.uri | http://hdl.handle.net/10722/292262 | - |
dc.description.abstract | We observed striking differences between the tumorigenic colony-forming cells present in the spleens of mice later after infection with the anemia-inducing strain of Friend leukemia virus (strain FV-A) and those present after infection with the polycythemia-inducing strain (strain FV-P). Cells within primary colonies derived from FV-A- and FV-P-transformed cells (CFU-FV-A and CFU-FV-P, respectively) contained hemoglobin and spectrin, indicating that the CFU-FV-A and CFU-FV-P were transformed erythroid progenitor cells. The proportion of cells containing hemoglobin was relatively high (>25%) in newly isolated cells lines derived from CFU-FV-P colonies, whereas cell lines derived from CFU-FV-A colonies had only low levels (0 to 2%) of hemoglobin-containing cells. A high proportion of the cell lines derived from CFU-FV-A colonies responded to pure erythropoietin and accumulated spectrin and hemoglobin, whereas the cell lines derived from CFU-FV-P colonies did not. A cytogenetic analysis indicated that primary CFU-FV-P colony cells were diploid, whereas chromosomal aberrations were observed in the immediate progeny of CFU-FV-A. The presence of unique chromosomal markers in the majority of the cells within individual colonies derived from CFU-FV-A suggested that these colonies originated from single cells. Finally, leukemic progenitor cells transformed by strain FV-A appeared to have an extensive capacity to self-renew (i.e., form secondary colonies in methylcellulose), whereas a significant proportion of the corresponding cells transformed by strain FV-P did not. In addition, the self-renewal capacity of both CFU-FV-A and CFU-FV-P increased as the disease progressed. From these observations, we propose a model for the multistage nature of Friend disease; this model involves clonal evolution and expansion from a differentiating population with limited proliferative capacity to a population with a high capacity for self-renewal and proliferation. | - |
dc.language | eng | - |
dc.relation.ispartof | Molecular and Cellular Biology | - |
dc.title | Clonal analysis of the late stages of erythroleukemia induced by two distinct strains of Friend leukemia virus | - |
dc.type | Article | - |
dc.description.nature | link_to_OA_fulltext | - |
dc.identifier.doi | 10.1128/MCB.1.8.721 | - |
dc.identifier.pmid | 9279385 | - |
dc.identifier.pmcid | PMC369352 | - |
dc.identifier.scopus | eid_2-s2.0-0019793666 | - |
dc.identifier.volume | 1 | - |
dc.identifier.issue | 8 | - |
dc.identifier.spage | 721 | - |
dc.identifier.epage | 730 | - |
dc.identifier.isi | WOS:A1981MA40900006 | - |
dc.identifier.issnl | 0270-7306 | - |