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Article: Specific ablation of the apoptotic functions of cytochrome c reveals a differential requirement for cytochrome c and Apaf-1 in apoptosis

TitleSpecific ablation of the apoptotic functions of cytochrome c reveals a differential requirement for cytochrome c and Apaf-1 in apoptosis
Authors
Issue Date2005
Citation
Cell, 2005, v. 121, n. 4, p. 579-591 How to Cite?
AbstractAs components of the apoptosome, a caspase-activating complex, cytochrome c (Cyt c) and Apaf-1 are thought to play critical roles during apoptosis. Due to the obligate function of Cyt c in electron transport, its requirement for apoptosis in animals has been difficult to establish. We generated "knockin" mice expressing a mutant Cyt c (KA allele), which retains normal electron transfer function but fails to activate Apaf-1. Most KA/KA mice displayed embryonic or perinatal lethality caused by defects in the central nervous system, and surviving mice exhibited impaired lymphocyte homeostasis. Although fibroblasts from the KA/KA mice were resistant to apoptosis, their thymocytes were markedly more sensitive to death stimuli than Apaf-1 -/- thymocytes. Upon treatment with γ irradiation, procaspases were efficiently activated in apoptotic KA/KA thymocytes, but Apaf-1 oligomerization was not observed. These studies indicate the existence of a Cyt c- and apoptosome-independent but Apaf-1-dependent mechanism(s) for caspase activation. Copyright ©2005 by Elsevier Inc.
Persistent Identifierhttp://hdl.handle.net/10722/292190
ISSN
2023 Impact Factor: 45.5
2023 SCImago Journal Rankings: 24.342
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorHao, Zhenyue-
dc.contributor.authorDuncan, Gordon S.-
dc.contributor.authorChang, Chia Che-
dc.contributor.authorElia, Andrew-
dc.contributor.authorFang, Min-
dc.contributor.authorWakeham, Andrew-
dc.contributor.authorOkada, Hitoshi-
dc.contributor.authorCalzascia, Thomas-
dc.contributor.authorJang, Yingju-
dc.contributor.authorYou-Ten, Annick-
dc.contributor.authorYeh, Wen Chen-
dc.contributor.authorOhashi, Pamela-
dc.contributor.authorWang, Xiaodong-
dc.contributor.authorMak, Tak W.-
dc.date.accessioned2020-11-17T14:55:57Z-
dc.date.available2020-11-17T14:55:57Z-
dc.date.issued2005-
dc.identifier.citationCell, 2005, v. 121, n. 4, p. 579-591-
dc.identifier.issn0092-8674-
dc.identifier.urihttp://hdl.handle.net/10722/292190-
dc.description.abstractAs components of the apoptosome, a caspase-activating complex, cytochrome c (Cyt c) and Apaf-1 are thought to play critical roles during apoptosis. Due to the obligate function of Cyt c in electron transport, its requirement for apoptosis in animals has been difficult to establish. We generated "knockin" mice expressing a mutant Cyt c (KA allele), which retains normal electron transfer function but fails to activate Apaf-1. Most KA/KA mice displayed embryonic or perinatal lethality caused by defects in the central nervous system, and surviving mice exhibited impaired lymphocyte homeostasis. Although fibroblasts from the KA/KA mice were resistant to apoptosis, their thymocytes were markedly more sensitive to death stimuli than Apaf-1 -/- thymocytes. Upon treatment with γ irradiation, procaspases were efficiently activated in apoptotic KA/KA thymocytes, but Apaf-1 oligomerization was not observed. These studies indicate the existence of a Cyt c- and apoptosome-independent but Apaf-1-dependent mechanism(s) for caspase activation. Copyright ©2005 by Elsevier Inc.-
dc.languageeng-
dc.relation.ispartofCell-
dc.titleSpecific ablation of the apoptotic functions of cytochrome c reveals a differential requirement for cytochrome c and Apaf-1 in apoptosis-
dc.typeArticle-
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1016/j.cell.2005.03.016-
dc.identifier.pmid15907471-
dc.identifier.scopuseid_2-s2.0-21144451097-
dc.identifier.volume121-
dc.identifier.issue4-
dc.identifier.spage579-
dc.identifier.epage591-
dc.identifier.isiWOS:000229331200014-
dc.identifier.f10001026105-
dc.identifier.issnl0092-8674-

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