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Article: Distinct roles for LFA-1 and CD28 during activation of naive T cells: Adhesion versus costimulation

TitleDistinct roles for LFA-1 and CD28 during activation of naive T cells: Adhesion versus costimulation
Authors
Issue Date1997
Citation
Immunity, 1997, v. 7, n. 4, p. 549-557 How to Cite?
AbstractEfficient T cell activation requires the engagement of a variety of ligand/receptor molecules in addition to T cell receptor (TCR)-major histocompatibility complex (MHC)/peptide interactions. The leukocyte function antigen 1 (LFA-1) and the CD28 glycoprotein have both been implicated in T cell activation. The present study dissects the roles of LFA-1 and CD28 in the activation of naive virus-specific CD8+ T cells. We demonstrate that LFA-1 facilitates T cell activation by lowering the amounts of antigen necessary for T cell activation. In the absence of LFA-1, 100-fold more antigen was required for T cell-antigen-presenting cell (APC) conjugation and all subsequent events of T cell activation, including TCR down-regulation, Ca2+-flux, T cell proliferation, and lytic effector cell induction. Thus, LFA-1 facilitates the function triggering of TCRs by promoting adhesion of T cells to APCs but does not effect T cell activation otherwise. In contrast, CD28 played an entirely different role during T cell activation. CD28 reduced the number of TCRs that had to be triggered for T cell activation and allowed activation of T cells by low affinity ligands. CD28 but not LFA-1 prevented induction of T cell unresponsiveness after stimulation of TCRs. These results demonstrate that LFA-1 and CD28 exhibit distinct, nonoverlapping ways to influence T cell activation and suggest that the terms costimulation and signal 2 should be revisited.
Persistent Identifierhttp://hdl.handle.net/10722/292173
ISSN
2023 Impact Factor: 25.5
2023 SCImago Journal Rankings: 13.578
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorBachmann, Martin F.-
dc.contributor.authorMcKall-Faienza, Kim-
dc.contributor.authorSchmits, Rudolf-
dc.contributor.authorBouchard, Denis-
dc.contributor.authorBeach, Janine-
dc.contributor.authorSpeiser, Daniel E.-
dc.contributor.authorMak, Tak W.-
dc.contributor.authorOhashi, Pamela S.-
dc.date.accessioned2020-11-17T14:55:55Z-
dc.date.available2020-11-17T14:55:55Z-
dc.date.issued1997-
dc.identifier.citationImmunity, 1997, v. 7, n. 4, p. 549-557-
dc.identifier.issn1074-7613-
dc.identifier.urihttp://hdl.handle.net/10722/292173-
dc.description.abstractEfficient T cell activation requires the engagement of a variety of ligand/receptor molecules in addition to T cell receptor (TCR)-major histocompatibility complex (MHC)/peptide interactions. The leukocyte function antigen 1 (LFA-1) and the CD28 glycoprotein have both been implicated in T cell activation. The present study dissects the roles of LFA-1 and CD28 in the activation of naive virus-specific CD8+ T cells. We demonstrate that LFA-1 facilitates T cell activation by lowering the amounts of antigen necessary for T cell activation. In the absence of LFA-1, 100-fold more antigen was required for T cell-antigen-presenting cell (APC) conjugation and all subsequent events of T cell activation, including TCR down-regulation, Ca2+-flux, T cell proliferation, and lytic effector cell induction. Thus, LFA-1 facilitates the function triggering of TCRs by promoting adhesion of T cells to APCs but does not effect T cell activation otherwise. In contrast, CD28 played an entirely different role during T cell activation. CD28 reduced the number of TCRs that had to be triggered for T cell activation and allowed activation of T cells by low affinity ligands. CD28 but not LFA-1 prevented induction of T cell unresponsiveness after stimulation of TCRs. These results demonstrate that LFA-1 and CD28 exhibit distinct, nonoverlapping ways to influence T cell activation and suggest that the terms costimulation and signal 2 should be revisited.-
dc.languageeng-
dc.relation.ispartofImmunity-
dc.titleDistinct roles for LFA-1 and CD28 during activation of naive T cells: Adhesion versus costimulation-
dc.typeArticle-
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1016/S1074-7613(00)80376-3-
dc.identifier.pmid9354475-
dc.identifier.scopuseid_2-s2.0-0030701952-
dc.identifier.volume7-
dc.identifier.issue4-
dc.identifier.spage549-
dc.identifier.epage557-
dc.identifier.isiWOS:A1997YD59300011-
dc.identifier.issnl1074-7613-

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