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- Publisher Website: 10.1016/S0896-6273(01)00263-X
- Scopus: eid_2-s2.0-0035025619
- PMID: 11343645
- WOS: WOS:000168412900011
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Article: Direct neural fate specification from embryonic stem cells: A primitive mammalian neural stem cell stage acquired through a default mechanism
Title | Direct neural fate specification from embryonic stem cells: A primitive mammalian neural stem cell stage acquired through a default mechanism |
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Authors | |
Issue Date | 2001 |
Citation | Neuron, 2001, v. 30, n. 1, p. 65-78 How to Cite? |
Abstract | Little is known about how neural stem cells are formed initially during development. We investigated whether a default mechanism of neural specification could regulate acquisition of neural stem cell identity directly from embryonic stem (ES) cells. ES cells cultured in defined, low-density conditions readily acquire a neural identity. We characterize a novel primitive neural stem cell as a component of neural lineage specification that is negatively regulated by TGFβ-related signaling. Primitive neural stem cells have distinct growth factor requirements, express neural precursor markers, generate neurons and glia in vitro, and have neural and non-neural lineage potential in vivo. These results are consistent with a default mechanism for neural fate specification and support a model whereby definitive neural stem cell formation is preceded by a primitive neural stem cell stage during neural lineage commitment. |
Persistent Identifier | http://hdl.handle.net/10722/291563 |
ISSN | 2023 Impact Factor: 14.7 2023 SCImago Journal Rankings: 7.728 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Tropepe, Vincent | - |
dc.contributor.author | Hitoshi, Seiji | - |
dc.contributor.author | Sirard, Christian | - |
dc.contributor.author | Mak, Tak W. | - |
dc.contributor.author | Rossant, Janet | - |
dc.contributor.author | Van Der Kooy, Derek | - |
dc.date.accessioned | 2020-11-17T14:54:38Z | - |
dc.date.available | 2020-11-17T14:54:38Z | - |
dc.date.issued | 2001 | - |
dc.identifier.citation | Neuron, 2001, v. 30, n. 1, p. 65-78 | - |
dc.identifier.issn | 0896-6273 | - |
dc.identifier.uri | http://hdl.handle.net/10722/291563 | - |
dc.description.abstract | Little is known about how neural stem cells are formed initially during development. We investigated whether a default mechanism of neural specification could regulate acquisition of neural stem cell identity directly from embryonic stem (ES) cells. ES cells cultured in defined, low-density conditions readily acquire a neural identity. We characterize a novel primitive neural stem cell as a component of neural lineage specification that is negatively regulated by TGFβ-related signaling. Primitive neural stem cells have distinct growth factor requirements, express neural precursor markers, generate neurons and glia in vitro, and have neural and non-neural lineage potential in vivo. These results are consistent with a default mechanism for neural fate specification and support a model whereby definitive neural stem cell formation is preceded by a primitive neural stem cell stage during neural lineage commitment. | - |
dc.language | eng | - |
dc.relation.ispartof | Neuron | - |
dc.title | Direct neural fate specification from embryonic stem cells: A primitive mammalian neural stem cell stage acquired through a default mechanism | - |
dc.type | Article | - |
dc.description.nature | link_to_OA_fulltext | - |
dc.identifier.doi | 10.1016/S0896-6273(01)00263-X | - |
dc.identifier.pmid | 11343645 | - |
dc.identifier.scopus | eid_2-s2.0-0035025619 | - |
dc.identifier.volume | 30 | - |
dc.identifier.issue | 1 | - |
dc.identifier.spage | 65 | - |
dc.identifier.epage | 78 | - |
dc.identifier.isi | WOS:000168412900011 | - |
dc.identifier.issnl | 0896-6273 | - |