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- Publisher Website: 10.1074/jbc.275.17.12821
- Scopus: eid_2-s2.0-0034724727
- PMID: 10777580
- WOS: WOS:000086762300064
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Article: Cloning and characterization of the murine glucosamine-6-phosphate acetyltransferase EMeg32: Differential expression and intracellular membrane association
Title | Cloning and characterization of the murine glucosamine-6-phosphate acetyltransferase EMeg32: Differential expression and intracellular membrane association |
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Authors | |
Issue Date | 2000 |
Citation | Journal of Biological Chemistry, 2000, v. 275, n. 17, p. 12821-12832 How to Cite? |
Abstract | N-Linked glycosylation is a post-translational modification occurring in many eukaryotic secreted and surface-bound proteins and has impact on diverse physiological and pathological processes. Similarly important is the generation of glycosylphosphatidylinositol linkers, which anchor membrane proteins to the cell. Both protein modifications depend on the central nucleotide sugar UDP-N-acetylglucosamine (UDP-GlcNAc). The enzymatic reactions leading to generation of nucleotide sugars are established, yet most of the respective genes still await cloning. We describe the characterization of such a gene, EMeg32, which we identified based on its differential expression in murine hematopoietic precursor cells. We further demonstrate regulated expression during embryogenesis. EMeg32 codes for a 184-amino acid protein exhibiting glucosamine-6-phosphate acetyltransferase activity. It thereby holds a key position in the pathway toward de novo UDP- GlcNAc synthesis. Surprisingly, the protein associates with the cytoplasmic side of various intracellular membranes, accumulates prior to mitosis, and copurifies with the cdc48 homolog p97/valosin-containing protein. |
Persistent Identifier | http://hdl.handle.net/10722/291549 |
ISSN | 2020 Impact Factor: 5.157 2023 SCImago Journal Rankings: 1.766 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Boehmelt, Guido | - |
dc.contributor.author | Fialka, Irene | - |
dc.contributor.author | Brothers, Greg | - |
dc.contributor.author | McGinley, Michael D. | - |
dc.contributor.author | Patterson, Scott D. | - |
dc.contributor.author | Mo, Rong | - |
dc.contributor.author | Hui, Chi Chung | - |
dc.contributor.author | Chung, Stephen | - |
dc.contributor.author | Huber, Lukas A. | - |
dc.contributor.author | Mak, Tak W. | - |
dc.contributor.author | Iscove, Norman N. | - |
dc.date.accessioned | 2020-11-17T14:54:36Z | - |
dc.date.available | 2020-11-17T14:54:36Z | - |
dc.date.issued | 2000 | - |
dc.identifier.citation | Journal of Biological Chemistry, 2000, v. 275, n. 17, p. 12821-12832 | - |
dc.identifier.issn | 0021-9258 | - |
dc.identifier.uri | http://hdl.handle.net/10722/291549 | - |
dc.description.abstract | N-Linked glycosylation is a post-translational modification occurring in many eukaryotic secreted and surface-bound proteins and has impact on diverse physiological and pathological processes. Similarly important is the generation of glycosylphosphatidylinositol linkers, which anchor membrane proteins to the cell. Both protein modifications depend on the central nucleotide sugar UDP-N-acetylglucosamine (UDP-GlcNAc). The enzymatic reactions leading to generation of nucleotide sugars are established, yet most of the respective genes still await cloning. We describe the characterization of such a gene, EMeg32, which we identified based on its differential expression in murine hematopoietic precursor cells. We further demonstrate regulated expression during embryogenesis. EMeg32 codes for a 184-amino acid protein exhibiting glucosamine-6-phosphate acetyltransferase activity. It thereby holds a key position in the pathway toward de novo UDP- GlcNAc synthesis. Surprisingly, the protein associates with the cytoplasmic side of various intracellular membranes, accumulates prior to mitosis, and copurifies with the cdc48 homolog p97/valosin-containing protein. | - |
dc.language | eng | - |
dc.relation.ispartof | Journal of Biological Chemistry | - |
dc.title | Cloning and characterization of the murine glucosamine-6-phosphate acetyltransferase EMeg32: Differential expression and intracellular membrane association | - |
dc.type | Article | - |
dc.description.nature | link_to_OA_fulltext | - |
dc.identifier.doi | 10.1074/jbc.275.17.12821 | - |
dc.identifier.pmid | 10777580 | - |
dc.identifier.scopus | eid_2-s2.0-0034724727 | - |
dc.identifier.volume | 275 | - |
dc.identifier.issue | 17 | - |
dc.identifier.spage | 12821 | - |
dc.identifier.epage | 12832 | - |
dc.identifier.isi | WOS:000086762300064 | - |
dc.identifier.issnl | 0021-9258 | - |