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- Publisher Website: 10.1101/gad.14.7.854
- Scopus: eid_2-s2.0-0034175632
- PMID: 10766741
- WOS: WOS:000086569000010
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Article: Severe liver degeneration and lack of NF-κB activation in NEMO/IKK γ- deficient mice
Title | Severe liver degeneration and lack of NF-κB activation in NEMO/IKK γ- deficient mice |
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Authors | |
Keywords | NEMO/IKKγ NF-γB IKK Liver apoptosis |
Issue Date | 2000 |
Citation | Genes and Development, 2000, v. 14, n. 7, p. 854-862 How to Cite? |
Abstract | Phosphorylation of IκB, an inhibitor of NF-κB, is an important step in the activation of the transcription factor NF-κB. Phosphorylation is mediated by the IκB kinase (IKK) complex, known to contain two catalytic subunits: IKKα and IKKβ. A novel, noncatalytic component of this kinase complex called NEMO (NF-κB essential modulator)/IKKγ was identified recently. We have generated NEMO/IKKγ-deficient mice by gene targeting. Mutant embryos die at E12.5-E13.0 from severe liver damage due to apoptosis. NEMO/IKKγ-deficient primary murine embryonic fibroblasts (MEFs) lack detectable NF-γB DNA-binding activity in response to TNFα, IL-1, LPS, and Poly(IC) and do not show stimulus-dependent IκB kinase activity, which correlates with a lack of phosphorylation and degradation of IκBα. Consistent with these data, mutant MEFs show increased sensitivity to TNFα- induced apoptosis. Our data provide in vivo evidence that NEMO/IKKγ is the first essential, noncatalytic component of the IKK complex. |
Persistent Identifier | http://hdl.handle.net/10722/291530 |
ISSN | 2023 Impact Factor: 7.5 2023 SCImago Journal Rankings: 5.015 |
PubMed Central ID | |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Rudolph, Dorothea | - |
dc.contributor.author | Yeh, Wen Chen | - |
dc.contributor.author | Wakeham, Andrew | - |
dc.contributor.author | Rudolph, Bettina | - |
dc.contributor.author | Nallainathan, Dhani | - |
dc.contributor.author | Potter, Julia | - |
dc.contributor.author | Elia, Andrew J. | - |
dc.contributor.author | Mak, Tak W. | - |
dc.date.accessioned | 2020-11-17T14:54:34Z | - |
dc.date.available | 2020-11-17T14:54:34Z | - |
dc.date.issued | 2000 | - |
dc.identifier.citation | Genes and Development, 2000, v. 14, n. 7, p. 854-862 | - |
dc.identifier.issn | 0890-9369 | - |
dc.identifier.uri | http://hdl.handle.net/10722/291530 | - |
dc.description.abstract | Phosphorylation of IκB, an inhibitor of NF-κB, is an important step in the activation of the transcription factor NF-κB. Phosphorylation is mediated by the IκB kinase (IKK) complex, known to contain two catalytic subunits: IKKα and IKKβ. A novel, noncatalytic component of this kinase complex called NEMO (NF-κB essential modulator)/IKKγ was identified recently. We have generated NEMO/IKKγ-deficient mice by gene targeting. Mutant embryos die at E12.5-E13.0 from severe liver damage due to apoptosis. NEMO/IKKγ-deficient primary murine embryonic fibroblasts (MEFs) lack detectable NF-γB DNA-binding activity in response to TNFα, IL-1, LPS, and Poly(IC) and do not show stimulus-dependent IκB kinase activity, which correlates with a lack of phosphorylation and degradation of IκBα. Consistent with these data, mutant MEFs show increased sensitivity to TNFα- induced apoptosis. Our data provide in vivo evidence that NEMO/IKKγ is the first essential, noncatalytic component of the IKK complex. | - |
dc.language | eng | - |
dc.relation.ispartof | Genes and Development | - |
dc.subject | NEMO/IKKγ | - |
dc.subject | NF-γB | - |
dc.subject | IKK | - |
dc.subject | Liver apoptosis | - |
dc.title | Severe liver degeneration and lack of NF-κB activation in NEMO/IKK γ- deficient mice | - |
dc.type | Article | - |
dc.description.nature | link_to_OA_fulltext | - |
dc.identifier.doi | 10.1101/gad.14.7.854 | - |
dc.identifier.pmid | 10766741 | - |
dc.identifier.pmcid | PMC316493 | - |
dc.identifier.scopus | eid_2-s2.0-0034175632 | - |
dc.identifier.volume | 14 | - |
dc.identifier.issue | 7 | - |
dc.identifier.spage | 854 | - |
dc.identifier.epage | 862 | - |
dc.identifier.isi | WOS:000086569000010 | - |
dc.identifier.issnl | 0890-9369 | - |