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Article: Plasma DNA as a prognostic marker in trauma patients

TitlePlasma DNA as a prognostic marker in trauma patients
Authors
Issue Date2000
Citation
Clinical Chemistry, 2000, v. 46, n. 3, p. 319-323 How to Cite?
AbstractBackground: Recently, much interest has developed in the potential use of plasma DNA as a diagnostic and monitoring tool. We hypothesized that plasma DNA is increased in patients with trauma and may be prognostic in such patients. Methods: We studied 84 patients who had sustained an acute blunt traumatic injury. We measured plasma DNA by a real-time quantitative PCR assay for the β-globin gene. Blood samples were collected at a median time of 60 min following injury. Blood samples were also obtained from 27 control subjects. Results: The median plasma DNA concentrations in the control, minor/moderate trauma (Injury Severity Score <16; n = 47), and major trauma (Injury Severity Score ≥16; n = 37) groups were 3154 kilogenome- equivalents/L, 13 818 kilogenome-equivalents/L, and 181 303 kilogenome- equivalents/L, respectively. Plasma DNA concentrations in patients with adverse outcomes, including acute lung injury, acute respiratory distress syndrome, and death, had 11.6- to 12-fold higher plasma DNA concentrations than those who did not develop these complications. At a cutoff of 232 719 kilogenome-equivalents/L, the sensitivities of plasma DNA analysis for the prediction of acute lung injury, acute respiratory distress syndrome, and death were 100% (95% confidence interval, 100-100%), 100% (95% confidence interval, 100- 100%), and 78% (95% confidence interval, 40-97%), respectively. The respective specificities were 81% (95% confidence interval, 71-89%), 80% (95% confidence interval, 70-88%), and 82% (95% confidence interval, 71-90%). Conclusions: Plasma DNA is increased after trauma and may be a potentially valuable prognostic marker for these patients. (C) 2000 American Association for Clinical Chemistry.
Persistent Identifierhttp://hdl.handle.net/10722/291526
ISSN
2023 Impact Factor: 7.1
2023 SCImago Journal Rankings: 1.460

 

DC FieldValueLanguage
dc.contributor.authorLo, Y. M.Dennis-
dc.contributor.authorRainer, Timothy H.-
dc.contributor.authorChan, Lisa Y.S.-
dc.contributor.authorHjelm, N. Magnus-
dc.contributor.authorCocks, Robert A.-
dc.date.accessioned2020-11-17T14:54:33Z-
dc.date.available2020-11-17T14:54:33Z-
dc.date.issued2000-
dc.identifier.citationClinical Chemistry, 2000, v. 46, n. 3, p. 319-323-
dc.identifier.issn0009-9147-
dc.identifier.urihttp://hdl.handle.net/10722/291526-
dc.description.abstractBackground: Recently, much interest has developed in the potential use of plasma DNA as a diagnostic and monitoring tool. We hypothesized that plasma DNA is increased in patients with trauma and may be prognostic in such patients. Methods: We studied 84 patients who had sustained an acute blunt traumatic injury. We measured plasma DNA by a real-time quantitative PCR assay for the β-globin gene. Blood samples were collected at a median time of 60 min following injury. Blood samples were also obtained from 27 control subjects. Results: The median plasma DNA concentrations in the control, minor/moderate trauma (Injury Severity Score <16; n = 47), and major trauma (Injury Severity Score ≥16; n = 37) groups were 3154 kilogenome- equivalents/L, 13 818 kilogenome-equivalents/L, and 181 303 kilogenome- equivalents/L, respectively. Plasma DNA concentrations in patients with adverse outcomes, including acute lung injury, acute respiratory distress syndrome, and death, had 11.6- to 12-fold higher plasma DNA concentrations than those who did not develop these complications. At a cutoff of 232 719 kilogenome-equivalents/L, the sensitivities of plasma DNA analysis for the prediction of acute lung injury, acute respiratory distress syndrome, and death were 100% (95% confidence interval, 100-100%), 100% (95% confidence interval, 100- 100%), and 78% (95% confidence interval, 40-97%), respectively. The respective specificities were 81% (95% confidence interval, 71-89%), 80% (95% confidence interval, 70-88%), and 82% (95% confidence interval, 71-90%). Conclusions: Plasma DNA is increased after trauma and may be a potentially valuable prognostic marker for these patients. (C) 2000 American Association for Clinical Chemistry.-
dc.languageeng-
dc.relation.ispartofClinical Chemistry-
dc.titlePlasma DNA as a prognostic marker in trauma patients-
dc.typeArticle-
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1093/clinchem/46.3.319-
dc.identifier.pmid10702517-
dc.identifier.scopuseid_2-s2.0-0034020737-
dc.identifier.volume46-
dc.identifier.issue3-
dc.identifier.spage319-
dc.identifier.epage323-
dc.identifier.issnl0009-9147-

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