File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Interleukin 13 is secreted by and stimulates the growth of Hodgkin and Reed-Sternberg cells

TitleInterleukin 13 is secreted by and stimulates the growth of Hodgkin and Reed-Sternberg cells
Authors
KeywordsGene expression
Lymphoma
Proliferation
Cytokines
cDNA microarray
Issue Date1999
Citation
Journal of Experimental Medicine, 1999, v. 189, n. 12, p. 1939-1946 How to Cite?
AbstractGene expression patterns can provide vital clues to the pathogenesis of neoplastic diseases. We investigated the expression of 950 genes in Hodgkin's disease (HD) by analyzing differential mRNA expression using microarrays. In two independent microarray experiments, the HD-derived cell lines L428 and KMH2 were compared with an Epstein-Barr virus (EBV)-immortalized lymphoblastoid B cell line, LCL-GK. Interleukin (IL)-13 and IL-5 were found to be highly expressed in the HD-derived cell lines. Examination of lL-13 and IL-5 expression by Northern blot analysis and enzyme-linked immunosorbent assay confirmed these results and revealed the expression of IL-13 in a third HD-derived cell line, HDLM2. Control LCL and EBV-negative non-Hodgkin lymphoma-derived cell lines did not express IL-13. In situ hybridization of lymph node tissue from HD patients showed that elevated levels of IL-13 were specifically expressed by Hodgkin/Reed-Sternberg (H/RS) tumor cells. Treatment of a HD-derived cell line with a neutralizing antibody to IL-13 resulted in a dose-dependent inhibition of H/RS cell proliferation. These data suggest that H/RS cells produce IL-13 and that IL-13 plays an important role in the stimulation of H/RS cell growth, possibly by an autocrine mechanism. Modulation of the IL-13 signaling pathway may be a logical objective for future therapeutic strategies.
Persistent Identifierhttp://hdl.handle.net/10722/291506
ISSN
2023 Impact Factor: 12.6
2023 SCImago Journal Rankings: 6.838
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorKapp, Ursula-
dc.contributor.authorYeh, Wen Chen-
dc.contributor.authorPatterson, Bruce-
dc.contributor.authorElia, Andrew J.-
dc.contributor.authorKägi, David-
dc.contributor.authorHo, Alexandra-
dc.contributor.authorHessel, Andrew-
dc.contributor.authorTipsword, Mike-
dc.contributor.authorWilliams, Alexia-
dc.contributor.authorMirtsos, Christine-
dc.contributor.authorItie, Annick-
dc.contributor.authorMoyle, Matthew-
dc.contributor.authorMak, Tak W.-
dc.date.accessioned2020-11-17T14:54:31Z-
dc.date.available2020-11-17T14:54:31Z-
dc.date.issued1999-
dc.identifier.citationJournal of Experimental Medicine, 1999, v. 189, n. 12, p. 1939-1946-
dc.identifier.issn0022-1007-
dc.identifier.urihttp://hdl.handle.net/10722/291506-
dc.description.abstractGene expression patterns can provide vital clues to the pathogenesis of neoplastic diseases. We investigated the expression of 950 genes in Hodgkin's disease (HD) by analyzing differential mRNA expression using microarrays. In two independent microarray experiments, the HD-derived cell lines L428 and KMH2 were compared with an Epstein-Barr virus (EBV)-immortalized lymphoblastoid B cell line, LCL-GK. Interleukin (IL)-13 and IL-5 were found to be highly expressed in the HD-derived cell lines. Examination of lL-13 and IL-5 expression by Northern blot analysis and enzyme-linked immunosorbent assay confirmed these results and revealed the expression of IL-13 in a third HD-derived cell line, HDLM2. Control LCL and EBV-negative non-Hodgkin lymphoma-derived cell lines did not express IL-13. In situ hybridization of lymph node tissue from HD patients showed that elevated levels of IL-13 were specifically expressed by Hodgkin/Reed-Sternberg (H/RS) tumor cells. Treatment of a HD-derived cell line with a neutralizing antibody to IL-13 resulted in a dose-dependent inhibition of H/RS cell proliferation. These data suggest that H/RS cells produce IL-13 and that IL-13 plays an important role in the stimulation of H/RS cell growth, possibly by an autocrine mechanism. Modulation of the IL-13 signaling pathway may be a logical objective for future therapeutic strategies.-
dc.languageeng-
dc.relation.ispartofJournal of Experimental Medicine-
dc.subjectGene expression-
dc.subjectLymphoma-
dc.subjectProliferation-
dc.subjectCytokines-
dc.subjectcDNA microarray-
dc.titleInterleukin 13 is secreted by and stimulates the growth of Hodgkin and Reed-Sternberg cells-
dc.typeArticle-
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1084/jem.189.12.1939-
dc.identifier.pmid10377189-
dc.identifier.pmcidPMC2192965-
dc.identifier.scopuseid_2-s2.0-0033591689-
dc.identifier.volume189-
dc.identifier.issue12-
dc.identifier.spage1939-
dc.identifier.epage1946-
dc.identifier.isiWOS:000081157000009-
dc.identifier.issnl0022-1007-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats