File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: CD28-independent, TRAF2-dependent costimulation of resting T cells by 4-1BB ligand

TitleCD28-independent, TRAF2-dependent costimulation of resting T cells by 4-1BB ligand
Authors
KeywordsCostimulation
TRAF2
Signaling
T cells
4-1BB
Issue Date1998
Citation
Journal of Experimental Medicine, 1998, v. 187, n. 11, p. 1849-1862 How to Cite?
Abstract4-1BB ligand (4-1BBL) is a member of the tumor necrosis factor (TNF) family expressed on activated antigen-presenting cells. Its receptor, 4-1BB, is a member of the TNF receptor family expressed on activated CD4 and CD8 T cells. We have produced a soluble form of 4-1BBL using the baculovirus expression system. When coimmobilized on plastic with anti-CD3, soluble 4- 1BBL induces interleukin (IL)-2 production by resting CD28+ or CD28- T cells, indicating that 4-1BBL can function independently of other cell surface molecules, including CD28, in costimulation of resting T cell activation. At low concentrations of anti-CD3, 4-1BBL is inferior to anti- CD28 in T cell activation. However, when 4-1BB ligand is provided together with strong TCR signals, then 4-1BBL and anti-CD28 are equally potent in stimulation of IL-2 production by resting T cells. We find that TNF receptor- associated factor (TRAF)1 or TRAF2 associate with a glutathione S- transferase-4-1BB cytoplasmic domain fusion protein in vitro. In T cells, we find that association of TRAF1 and TRAF2 with 4-1BB requires 4-1BB cross- linking. In support of a functional role for TRAF2 in 4-1BB signaling, we find that resting T cells isolated from TRAF2-deficient mice or from mice expressing a dominant negative form of TRAF2 fail to augment IL-2 production in response to soluble 4-1BBL. Thus 4-1BB, via the TRAF2 molecule, can provide CD28-independent costimulatory signals to resting T cells.
Persistent Identifierhttp://hdl.handle.net/10722/291451
ISSN
2023 Impact Factor: 12.6
2023 SCImago Journal Rankings: 6.838
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorSaoulli, Katina-
dc.contributor.authorLee, Soo Young-
dc.contributor.authorCannons, Jennifer L.-
dc.contributor.authorYeh, Wen Chen-
dc.contributor.authorSantana, Angela-
dc.contributor.authorGoldstein, Marni D.-
dc.contributor.authorBangia, Naveen-
dc.contributor.authorDeBenedette, Mark A.-
dc.contributor.authorMak, Tak W.-
dc.contributor.authorChoi, Yongwon-
dc.contributor.authorWatts, Tania H.-
dc.date.accessioned2020-11-17T14:54:24Z-
dc.date.available2020-11-17T14:54:24Z-
dc.date.issued1998-
dc.identifier.citationJournal of Experimental Medicine, 1998, v. 187, n. 11, p. 1849-1862-
dc.identifier.issn0022-1007-
dc.identifier.urihttp://hdl.handle.net/10722/291451-
dc.description.abstract4-1BB ligand (4-1BBL) is a member of the tumor necrosis factor (TNF) family expressed on activated antigen-presenting cells. Its receptor, 4-1BB, is a member of the TNF receptor family expressed on activated CD4 and CD8 T cells. We have produced a soluble form of 4-1BBL using the baculovirus expression system. When coimmobilized on plastic with anti-CD3, soluble 4- 1BBL induces interleukin (IL)-2 production by resting CD28+ or CD28- T cells, indicating that 4-1BBL can function independently of other cell surface molecules, including CD28, in costimulation of resting T cell activation. At low concentrations of anti-CD3, 4-1BBL is inferior to anti- CD28 in T cell activation. However, when 4-1BB ligand is provided together with strong TCR signals, then 4-1BBL and anti-CD28 are equally potent in stimulation of IL-2 production by resting T cells. We find that TNF receptor- associated factor (TRAF)1 or TRAF2 associate with a glutathione S- transferase-4-1BB cytoplasmic domain fusion protein in vitro. In T cells, we find that association of TRAF1 and TRAF2 with 4-1BB requires 4-1BB cross- linking. In support of a functional role for TRAF2 in 4-1BB signaling, we find that resting T cells isolated from TRAF2-deficient mice or from mice expressing a dominant negative form of TRAF2 fail to augment IL-2 production in response to soluble 4-1BBL. Thus 4-1BB, via the TRAF2 molecule, can provide CD28-independent costimulatory signals to resting T cells.-
dc.languageeng-
dc.relation.ispartofJournal of Experimental Medicine-
dc.subjectCostimulation-
dc.subjectTRAF2-
dc.subjectSignaling-
dc.subjectT cells-
dc.subject4-1BB-
dc.titleCD28-independent, TRAF2-dependent costimulation of resting T cells by 4-1BB ligand-
dc.typeArticle-
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1084/jem.187.11.1849-
dc.identifier.pmid9607925-
dc.identifier.pmcidPMC2212301-
dc.identifier.scopuseid_2-s2.0-0032101119-
dc.identifier.volume187-
dc.identifier.issue11-
dc.identifier.spage1849-
dc.identifier.epage1862-
dc.identifier.isiWOS:000074120200012-
dc.identifier.issnl0022-1007-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats