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Article: Cloning of human lymphocyte-specific interferon regulatory factor (hLSIRF/hIRF4) and mapping of the gene to 6p23-p25

TitleCloning of human lymphocyte-specific interferon regulatory factor (hLSIRF/hIRF4) and mapping of the gene to 6p23-p25
Authors
Issue Date1996
Citation
Genomics, 1996, v. 37, n. 2, p. 229-233 How to Cite?
AbstractThe interferon regulatory factor (IRF) genes encode a family of transcription factors involved in the transcriptional regulation of interferon and the interferon stimulated genes through recognition of the interferon stimulated response element. We previously reported the cloning of a murine lymphocyte-specific IRF (mLSIRF), which was rapidly induced following B- or T-cell receptor crosslinking. To study the role of LSIRF in human lymphocyte development, we have cloned the complete 5.3-kb cDNA for the human homolog (hLSIRF). hLSIRF is a protein of 450 amino acids with a predicted molecular weight of 51.6 kDa and possesses 92% identity at the amino acid level to mLSIRF, including near identity in the DNA-binding domain. In Northern blot analysis, a single transcript of ~5 kb was highly expressed in spleen and peripheral blood lymphocyte. hLSIRF mRNA was rapidly induced in peripheral T cells after crosslinking the T-cell receptor. Analysis of tumor cell lines showed that hLSIRF mRNA was basally expressed in most B- but not T-cell lines. Surprisingly hLSIRF mRNA was also found in the melanoma line G361 and is expressed in normal melanocytes as well. Sequence from a genomic clone for hLSIRF was compared to that from mouse and revealed an identical exon-intron structure and a conserved PU.1-binding motif in the promoter. By FISH analysis, hLSIRF was mapped to 6p23-p25.
Persistent Identifierhttp://hdl.handle.net/10722/291388
ISSN
2023 Impact Factor: 3.4
2023 SCImago Journal Rankings: 0.850
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorGrossman, Alex-
dc.contributor.authorMittrücker, Hans Willi-
dc.contributor.authorNicholl, Jillian-
dc.contributor.authorSuzuki, Akira-
dc.contributor.authorChung, Stephen-
dc.contributor.authorAntonio, Laarni-
dc.contributor.authorSuggs, Sid-
dc.contributor.authorSutherland, Grant R.-
dc.contributor.authorSiderovski, David P.-
dc.contributor.authorMak, Tak W.-
dc.date.accessioned2020-11-17T14:54:15Z-
dc.date.available2020-11-17T14:54:15Z-
dc.date.issued1996-
dc.identifier.citationGenomics, 1996, v. 37, n. 2, p. 229-233-
dc.identifier.issn0888-7543-
dc.identifier.urihttp://hdl.handle.net/10722/291388-
dc.description.abstractThe interferon regulatory factor (IRF) genes encode a family of transcription factors involved in the transcriptional regulation of interferon and the interferon stimulated genes through recognition of the interferon stimulated response element. We previously reported the cloning of a murine lymphocyte-specific IRF (mLSIRF), which was rapidly induced following B- or T-cell receptor crosslinking. To study the role of LSIRF in human lymphocyte development, we have cloned the complete 5.3-kb cDNA for the human homolog (hLSIRF). hLSIRF is a protein of 450 amino acids with a predicted molecular weight of 51.6 kDa and possesses 92% identity at the amino acid level to mLSIRF, including near identity in the DNA-binding domain. In Northern blot analysis, a single transcript of ~5 kb was highly expressed in spleen and peripheral blood lymphocyte. hLSIRF mRNA was rapidly induced in peripheral T cells after crosslinking the T-cell receptor. Analysis of tumor cell lines showed that hLSIRF mRNA was basally expressed in most B- but not T-cell lines. Surprisingly hLSIRF mRNA was also found in the melanoma line G361 and is expressed in normal melanocytes as well. Sequence from a genomic clone for hLSIRF was compared to that from mouse and revealed an identical exon-intron structure and a conserved PU.1-binding motif in the promoter. By FISH analysis, hLSIRF was mapped to 6p23-p25.-
dc.languageeng-
dc.relation.ispartofGenomics-
dc.titleCloning of human lymphocyte-specific interferon regulatory factor (hLSIRF/hIRF4) and mapping of the gene to 6p23-p25-
dc.typeArticle-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1006/geno.1996.0547-
dc.identifier.pmid8921401-
dc.identifier.scopuseid_2-s2.0-0030588140-
dc.identifier.volume37-
dc.identifier.issue2-
dc.identifier.spage229-
dc.identifier.epage233-
dc.identifier.isiWOS:A1996VN66700012-
dc.identifier.issnl0888-7543-

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