File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: A study of human neutrophil antigen genotype frequencies in Hong Kong

TitleA study of human neutrophil antigen genotype frequencies in Hong Kong
Authors
Keywordsallele frequencies
gene frequencies
geographical difference
human neutrophil antigens
Issue Date2018
PublisherWiley-Blackwell Publishing Ltd. The Journal's web site is located at http://onlinelibrary.wiley.com/journal/10.1111/(ISSN)1365-3148
Citation
Transfusion Medicine, 2018, v. 28 n. 4, p. 310-318 How to Cite?
AbstractBackground Alloantibodies against human neutrophil antigens (HNA) are associated with a variety of clinical conditions. Over the past decade, the allelic and genotypic frequencies of the five HNA systems have been evaluated. Although the HNA system is less polymorphic than human leukocyte antigens (HLA), significant differences in the genotypic and allele frequencies still exist in different populations, even those living in close proximity. Objectives To delineate HNA genotypic and allele frequencies to provide vital information on estimating the risk of HNA‐associated diseases for our local population. Methods Using a validated, in‐house‐developed assay, genotyping for HNA‐1, HNA‐3, HLA‐4 and HNA‐5 was performed on 300 samples from Chinese blood donors from Hong Kong. In addition, the frequency of the HNA‐2 c.843A > T allele was also determined. Results The allele frequencies of HNA‐1a, ‐1b and ‐1c alleles were 67·8, 31·5 and 0%, respectively, whereas the frequencies of HNA‐3a and HNA‐3b were 71·0 and 29·0%, respectively. The frequencies of HNA‐4a and ‐4b alleles were 99·5 and 0·5%, respectively, and for HNA‐5a and ‐5b, alleles were 85·2 and 14·8%, respectively. Homozygotes for the HNA‐2 c.843 TT variant were absent in our population, whereas only <4% of the population were c.843AT heterozygote carriers. Conclusions This is the first study to define HNA genotype and allele frequencies using a validated modified in‐house PCR‐SSP method in the Hong Kong Chinese blood donor population. Our approach provides a cost‐effective assay for conducting routine HNA typing and facilitates the incorporation of these assays into routine clinical service. Our results are comparable with those reported in the Guangzhou Chinese population, but the allele frequencies in our Hong Kong Chinese population are significantly different from the reported European frequencies, confirming that a geographical difference exists for HNA allele frequencies.
Persistent Identifierhttp://hdl.handle.net/10722/290634
ISSN
2023 Impact Factor: 1.5
2023 SCImago Journal Rankings: 0.451
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorTam, K-
dc.contributor.authorTang, I-
dc.contributor.authorYeung, W-
dc.contributor.authorLee, CK-
dc.contributor.authorIp, P-
dc.contributor.authorKwok, J-
dc.contributor.authorHo, J-
dc.date.accessioned2020-11-02T05:44:59Z-
dc.date.available2020-11-02T05:44:59Z-
dc.date.issued2018-
dc.identifier.citationTransfusion Medicine, 2018, v. 28 n. 4, p. 310-318-
dc.identifier.issn0958-7578-
dc.identifier.urihttp://hdl.handle.net/10722/290634-
dc.description.abstractBackground Alloantibodies against human neutrophil antigens (HNA) are associated with a variety of clinical conditions. Over the past decade, the allelic and genotypic frequencies of the five HNA systems have been evaluated. Although the HNA system is less polymorphic than human leukocyte antigens (HLA), significant differences in the genotypic and allele frequencies still exist in different populations, even those living in close proximity. Objectives To delineate HNA genotypic and allele frequencies to provide vital information on estimating the risk of HNA‐associated diseases for our local population. Methods Using a validated, in‐house‐developed assay, genotyping for HNA‐1, HNA‐3, HLA‐4 and HNA‐5 was performed on 300 samples from Chinese blood donors from Hong Kong. In addition, the frequency of the HNA‐2 c.843A > T allele was also determined. Results The allele frequencies of HNA‐1a, ‐1b and ‐1c alleles were 67·8, 31·5 and 0%, respectively, whereas the frequencies of HNA‐3a and HNA‐3b were 71·0 and 29·0%, respectively. The frequencies of HNA‐4a and ‐4b alleles were 99·5 and 0·5%, respectively, and for HNA‐5a and ‐5b, alleles were 85·2 and 14·8%, respectively. Homozygotes for the HNA‐2 c.843 TT variant were absent in our population, whereas only <4% of the population were c.843AT heterozygote carriers. Conclusions This is the first study to define HNA genotype and allele frequencies using a validated modified in‐house PCR‐SSP method in the Hong Kong Chinese blood donor population. Our approach provides a cost‐effective assay for conducting routine HNA typing and facilitates the incorporation of these assays into routine clinical service. Our results are comparable with those reported in the Guangzhou Chinese population, but the allele frequencies in our Hong Kong Chinese population are significantly different from the reported European frequencies, confirming that a geographical difference exists for HNA allele frequencies.-
dc.languageeng-
dc.publisherWiley-Blackwell Publishing Ltd. The Journal's web site is located at http://onlinelibrary.wiley.com/journal/10.1111/(ISSN)1365-3148-
dc.relation.ispartofTransfusion Medicine-
dc.rightsPreprint This is the pre-peer reviewed version of the following article: [FULL CITE], which has been published in final form at [Link to final article using the DOI]. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. Postprint This is the peer reviewed version of the following article: [FULL CITE], which has been published in final form at [Link to final article using the DOI]. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.-
dc.subjectallele frequencies-
dc.subjectgene frequencies-
dc.subjectgeographical difference-
dc.subjecthuman neutrophil antigens-
dc.titleA study of human neutrophil antigen genotype frequencies in Hong Kong-
dc.typeArticle-
dc.identifier.emailIp, P: patricip@hku.hk-
dc.identifier.authorityIp, P=rp01337-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1111/tme.12494-
dc.identifier.pmid29280200-
dc.identifier.scopuseid_2-s2.0-85039156880-
dc.identifier.hkuros318396-
dc.identifier.volume28-
dc.identifier.issue4-
dc.identifier.spage310-
dc.identifier.epage318-
dc.identifier.isiWOS:000441578700007-
dc.publisher.placeUnited Kingdom-
dc.identifier.issnl0958-7578-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats