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Article: One-step immunoassay of C-reactive protein using droplet microfluidics

TitleOne-step immunoassay of C-reactive protein using droplet microfluidics
Authors
Keywordsantigen antibody complex
automation
conjugation
diffusion
droplet microfluidics
Issue Date2016
PublisherRoyal Society of Chemistry. The Journal's web site is located at http://www.rsc.org/loc
Citation
Lab On a Chip, 2016, v. 16 n. 22, p. 4359-4365 How to Cite?
AbstractWe present a wash-free high-sensitivity immunoassay of C-reactive proteins with droplet microfluidics. Microbeads are encapsulated within droplets for the immunoassay, and the droplets are scanned by a fluorescence detection platform to quantify the amount of proteins captured on the microbeads. The limit of detection determined by our platform is 0.01 μg mL−1, which is ten times more sensitive than conventional high-sensitivity C-reactive protein assays. With the decrease in diffusion distance within droplets, the immunoassay requires only half of the time required for similar conventional approaches. This approach for carrying out immunoassays can potentially be applied to other biomarkers beyond C-reactive proteins.
Persistent Identifierhttp://hdl.handle.net/10722/290588
ISSN
2019 Impact Factor: 6.774
2015 SCImago Journal Rankings: 2.373
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorTANG, MYH-
dc.contributor.authorShum, HC-
dc.date.accessioned2020-11-02T05:44:22Z-
dc.date.available2020-11-02T05:44:22Z-
dc.date.issued2016-
dc.identifier.citationLab On a Chip, 2016, v. 16 n. 22, p. 4359-4365-
dc.identifier.issn1473-0197-
dc.identifier.urihttp://hdl.handle.net/10722/290588-
dc.description.abstractWe present a wash-free high-sensitivity immunoassay of C-reactive proteins with droplet microfluidics. Microbeads are encapsulated within droplets for the immunoassay, and the droplets are scanned by a fluorescence detection platform to quantify the amount of proteins captured on the microbeads. The limit of detection determined by our platform is 0.01 μg mL−1, which is ten times more sensitive than conventional high-sensitivity C-reactive protein assays. With the decrease in diffusion distance within droplets, the immunoassay requires only half of the time required for similar conventional approaches. This approach for carrying out immunoassays can potentially be applied to other biomarkers beyond C-reactive proteins.-
dc.languageeng-
dc.publisherRoyal Society of Chemistry. The Journal's web site is located at http://www.rsc.org/loc-
dc.relation.ispartofLab On a Chip-
dc.subjectantigen antibody complex-
dc.subjectautomation-
dc.subjectconjugation-
dc.subjectdiffusion-
dc.subjectdroplet microfluidics-
dc.titleOne-step immunoassay of C-reactive protein using droplet microfluidics-
dc.typeArticle-
dc.identifier.emailShum, HC: ashum@hku.hk-
dc.identifier.authorityShum, HC=rp01439-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1039/C6LC01121G-
dc.identifier.pmid27738692-
dc.identifier.scopuseid_2-s2.0-84994339122-
dc.identifier.hkuros317641-
dc.identifier.volume16-
dc.identifier.issue22-
dc.identifier.spage4359-
dc.identifier.epage4365-
dc.identifier.isiWOS:000387865000010-
dc.publisher.placeUnited Kingdom-
dc.identifier.issnl1473-0189-

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