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Conference Paper: The immunological roles of human γδ-T cells in Epstein-Barr virus infection-induced diseases.

TitleThe immunological roles of human γδ-T cells in Epstein-Barr virus infection-induced diseases.
Authors
Issue Date2019
PublisherWiley for European Federation of Immunological Societies (EFIS). The Journal's web site is located at http://onlinelibrary.wiley.com/journal/10.1002/(ISSN)1521-4141
Citation
17th International Congress of Immunology, International Union of Immunological Societies (IUIS), Beijing, China, 19-23 October 2019. Abstracts of IUIS 2019 Beijing in European Journal of Immunology, 2019, v. 49 n. Suppl. 3, p. 996 How to Cite?
AbstractEpstein-Barr virus (EBV) is a herpesvirus that latently infects human B cells in most individuals and a leading cause of several human malignancies, such as EBV-induced B cell lymphoproliferative disorders (EBV-LPD). Human Vγ9Vδ2-T cells represent a minor but crucial population in immune system and act as an important component of immune effector cells that contribute to immunosurveillance against viral infection. However, the immunological roles of Vγ9Vδ2-T cells in EBV infection and EBV-related disorders are not well studied and characterized. In our study, we demonstrated that phosphoantigen-activated human Vγ9Vδ2 -T cells have potent cytotoxic activity against EBV-transformed B lymphoblastoid cell lines (EBV-LCLs) through TCR γδ and NKG2D triggering, and FasL and TRAIL engagement. By targeting human Vγ9Vδ2-T cells, we further demonstrated that pamidronate can control EBV-LPD in humanized mice through boosting Vγ9Vδ2-T cell immunity. Furthermore, the human macrophage plays a very critical role in triggering the immune surveillance against the EBV infection. But, the relationship between the Vγ9Vδ2-T cells and macrophages is still unknown during EBV infection. Accordingly, our data showed that human Monocyte-driven-Macrophages (MDMs) could be infected by EBV. Subsequently, Vγ9Vδ2-T cells displayed potential cytotoxic activities against EBV-infected MDMs in vitro. Using the blocking experiment, the result demonstrated that special monoclonal antibodies against TCR γδ and NKG2D could significantly inhibit the killing of infected-MDMs by Vγ9Vδ2-T cells. Moreover, Vγ9Vδ2-T cells could impair viral replication in the infected-MDMs through producing IFN-γ. These findings provide proof-of-principle for a therapeutic approach using Vγ9Vδ2-T cells to control EBV infection and EBVassociated human malignancies.
DescriptionOral presentation - no. O200
Persistent Identifierhttp://hdl.handle.net/10722/290231
ISSN
2023 Impact Factor: 4.5
2023 SCImago Journal Rankings: 1.627

 

DC FieldValueLanguage
dc.contributor.authorXiang, Z-
dc.contributor.authorLiu, Y-
dc.contributor.authorLam, KT-
dc.contributor.authorWang, X-
dc.contributor.authorMu, X-
dc.contributor.authorWen, K-
dc.contributor.authorPei, Y-
dc.contributor.authorTu, W-
dc.date.accessioned2020-10-22T08:23:52Z-
dc.date.available2020-10-22T08:23:52Z-
dc.date.issued2019-
dc.identifier.citation17th International Congress of Immunology, International Union of Immunological Societies (IUIS), Beijing, China, 19-23 October 2019. Abstracts of IUIS 2019 Beijing in European Journal of Immunology, 2019, v. 49 n. Suppl. 3, p. 996-
dc.identifier.issn0014-2980-
dc.identifier.urihttp://hdl.handle.net/10722/290231-
dc.descriptionOral presentation - no. O200-
dc.description.abstractEpstein-Barr virus (EBV) is a herpesvirus that latently infects human B cells in most individuals and a leading cause of several human malignancies, such as EBV-induced B cell lymphoproliferative disorders (EBV-LPD). Human Vγ9Vδ2-T cells represent a minor but crucial population in immune system and act as an important component of immune effector cells that contribute to immunosurveillance against viral infection. However, the immunological roles of Vγ9Vδ2-T cells in EBV infection and EBV-related disorders are not well studied and characterized. In our study, we demonstrated that phosphoantigen-activated human Vγ9Vδ2 -T cells have potent cytotoxic activity against EBV-transformed B lymphoblastoid cell lines (EBV-LCLs) through TCR γδ and NKG2D triggering, and FasL and TRAIL engagement. By targeting human Vγ9Vδ2-T cells, we further demonstrated that pamidronate can control EBV-LPD in humanized mice through boosting Vγ9Vδ2-T cell immunity. Furthermore, the human macrophage plays a very critical role in triggering the immune surveillance against the EBV infection. But, the relationship between the Vγ9Vδ2-T cells and macrophages is still unknown during EBV infection. Accordingly, our data showed that human Monocyte-driven-Macrophages (MDMs) could be infected by EBV. Subsequently, Vγ9Vδ2-T cells displayed potential cytotoxic activities against EBV-infected MDMs in vitro. Using the blocking experiment, the result demonstrated that special monoclonal antibodies against TCR γδ and NKG2D could significantly inhibit the killing of infected-MDMs by Vγ9Vδ2-T cells. Moreover, Vγ9Vδ2-T cells could impair viral replication in the infected-MDMs through producing IFN-γ. These findings provide proof-of-principle for a therapeutic approach using Vγ9Vδ2-T cells to control EBV infection and EBVassociated human malignancies.-
dc.languageeng-
dc.publisherWiley for European Federation of Immunological Societies (EFIS). The Journal's web site is located at http://onlinelibrary.wiley.com/journal/10.1002/(ISSN)1521-4141-
dc.relation.ispartofEuropean Journal of Immunology-
dc.relation.ispartof17th International Congress of Immunology, 2019-
dc.titleThe immunological roles of human γδ-T cells in Epstein-Barr virus infection-induced diseases.-
dc.typeConference_Paper-
dc.identifier.emailTu, W: wwtu@hku.hk-
dc.identifier.authorityTu, W=rp00416-
dc.description.natureabstract-
dc.identifier.hkuros316639-
dc.identifier.volume49-
dc.identifier.issueSuppl. 3-
dc.identifier.spage996-
dc.identifier.epage996-
dc.publisher.placeGermany-
dc.identifier.partofdoi10.1002/eji.201970400-
dc.identifier.issnl0014-2980-

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