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Article: The efficacy of vacuum-ultraviolet light disinfection of some common environmental pathogens

TitleThe efficacy of vacuum-ultraviolet light disinfection of some common environmental pathogens
Authors
KeywordsDisinfection
Microorganism
Ozone
VUV
IAQ
Issue Date2020
PublisherBioMed Central Ltd. The Journal's web site is located at http://www.biomedcentral.com/bmcinfectdis/
Citation
BMC Infectious Diseases, 2020, v. 20, p. article no. 127 How to Cite?
AbstractBackground: This study is to elucidate the disinfection effect of ozone producing low-pressure Hg vapor lamps against human pathogens. Ozone producing low-pressure Hg vapor lamps emit mainly 254 nm ultraviolet light C (UVC) with about 10% power of Vacuum-ultraviolet (VUV) light at 185 nm. The combination of UVC and VUV can inactivate airborne pathogens by disrupting the genetic materials or generation of reactive oxygen species, respectively. In this study, inactivation of common bacteria including Escherichia coli ATCC25922 (E. coli), Extended Spectrum Beta-Lactamase-producing E. coli (ESBL), Methicillin-resistant Staphylococcus aureus (MRSA) and Mycobacterium tuberculosis (MTB), and that of influenza A viruses H1N1 and H3N2 under the radiation from ozone producing low-pressure Hg vapor lamps was examined. Log reduction values at different treatment durations were determined. Methods: In vitro tests were carried out. Various bacterium and virus suspensions were added onto nitrocellulose filter papers and subjected to the illumination from ozone producing low-pressure Hg vapor lamps. The extents of pathogen inactivation at different illumination times were investigated by conducting a series of experiments with increasing duration of illumination. log10 reduction in CFU/ml and reduction at log10(TCID50) were respectively measured for bacteria and viruses. The disinfection effectiveness of this type of lamps against the pathogens under the environment with a moderate barrier to light was therefore evaluated. Results: Ozone producing low-pressure Hg vapor lamp successfully inactivated these human pathogens. Nevertheless, among these pathogens, disinfection of MTB required more intense treatment. In the best tested situation, 3-log10 inactivation of pathogens can be achieved with ≤10 min of VUV treatment except MTB which needed about 20 min. This demonstrated the high resistance against UV disinfection of MTB. Conclusions: Following the criteria that valid germicidal results can be reflected with 3-log10 inactivation for bacteria, 4-log10 inactivation for viruses and 5-log10 inactivation for MTB, most of the bacteria required ≤10 min of VUV treatment, 20 min for the influenza viruses while MTB needed about 30 min VUV treatment. This indicated that VUV light is an effective approach against different environmental microorganisms.
Persistent Identifierhttp://hdl.handle.net/10722/290192
ISSN
2023 Impact Factor: 3.4
2023 SCImago Journal Rankings: 1.031
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorSzeto, W-
dc.contributor.authorYam, WC-
dc.contributor.authorHuang, H-
dc.contributor.authorLeung, DYC-
dc.date.accessioned2020-10-22T08:23:20Z-
dc.date.available2020-10-22T08:23:20Z-
dc.date.issued2020-
dc.identifier.citationBMC Infectious Diseases, 2020, v. 20, p. article no. 127-
dc.identifier.issn1471-2334-
dc.identifier.urihttp://hdl.handle.net/10722/290192-
dc.description.abstractBackground: This study is to elucidate the disinfection effect of ozone producing low-pressure Hg vapor lamps against human pathogens. Ozone producing low-pressure Hg vapor lamps emit mainly 254 nm ultraviolet light C (UVC) with about 10% power of Vacuum-ultraviolet (VUV) light at 185 nm. The combination of UVC and VUV can inactivate airborne pathogens by disrupting the genetic materials or generation of reactive oxygen species, respectively. In this study, inactivation of common bacteria including Escherichia coli ATCC25922 (E. coli), Extended Spectrum Beta-Lactamase-producing E. coli (ESBL), Methicillin-resistant Staphylococcus aureus (MRSA) and Mycobacterium tuberculosis (MTB), and that of influenza A viruses H1N1 and H3N2 under the radiation from ozone producing low-pressure Hg vapor lamps was examined. Log reduction values at different treatment durations were determined. Methods: In vitro tests were carried out. Various bacterium and virus suspensions were added onto nitrocellulose filter papers and subjected to the illumination from ozone producing low-pressure Hg vapor lamps. The extents of pathogen inactivation at different illumination times were investigated by conducting a series of experiments with increasing duration of illumination. log10 reduction in CFU/ml and reduction at log10(TCID50) were respectively measured for bacteria and viruses. The disinfection effectiveness of this type of lamps against the pathogens under the environment with a moderate barrier to light was therefore evaluated. Results: Ozone producing low-pressure Hg vapor lamp successfully inactivated these human pathogens. Nevertheless, among these pathogens, disinfection of MTB required more intense treatment. In the best tested situation, 3-log10 inactivation of pathogens can be achieved with ≤10 min of VUV treatment except MTB which needed about 20 min. This demonstrated the high resistance against UV disinfection of MTB. Conclusions: Following the criteria that valid germicidal results can be reflected with 3-log10 inactivation for bacteria, 4-log10 inactivation for viruses and 5-log10 inactivation for MTB, most of the bacteria required ≤10 min of VUV treatment, 20 min for the influenza viruses while MTB needed about 30 min VUV treatment. This indicated that VUV light is an effective approach against different environmental microorganisms.-
dc.languageeng-
dc.publisherBioMed Central Ltd. The Journal's web site is located at http://www.biomedcentral.com/bmcinfectdis/-
dc.relation.ispartofBMC Infectious Diseases-
dc.rightsBMC Infectious Diseases. Copyright © BioMed Central Ltd.-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.subjectDisinfection-
dc.subjectMicroorganism-
dc.subjectOzone-
dc.subjectVUV-
dc.subjectIAQ-
dc.titleThe efficacy of vacuum-ultraviolet light disinfection of some common environmental pathogens-
dc.typeArticle-
dc.identifier.emailYam, WC: wcyam@hku.hk-
dc.identifier.emailLeung, DYC: ycleung@hku.hk-
dc.identifier.authorityYam, WC=rp00313-
dc.identifier.authorityLeung, DYC=rp00149-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1186/s12879-020-4847-9-
dc.identifier.pmid32046660-
dc.identifier.pmcidPMC7014767-
dc.identifier.scopuseid_2-s2.0-85079339000-
dc.identifier.hkuros317179-
dc.identifier.volume20-
dc.identifier.spagearticle no. 127-
dc.identifier.epagearticle no. 127-
dc.identifier.isiWOS:000514609500006-
dc.publisher.placeUnited Kingdom-
dc.identifier.issnl1471-2334-

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