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Article: Early-Morning vs Spot Posterior Oropharyngeal Saliva for Diagnosis of SARS-CoV-2 Infection: Implication of Timing of Specimen Collection for Community-Wide Screening

TitleEarly-Morning vs Spot Posterior Oropharyngeal Saliva for Diagnosis of SARS-CoV-2 Infection: Implication of Timing of Specimen Collection for Community-Wide Screening
Authors
Keywordscoronavirus
diagnosis
SARS-CoV-2
saliva
screening
Issue Date2020
PublisherOxford University Press (OUP): Policy C. The Journal's web site is located at http://ofid.oxfordjournals.org/
Citation
Open Forum Infectious Diseases, 2020, v. 7 n. 6, article no. ofaa210 How to Cite?
AbstractBackground: Posterior oropharyngeal saliva is increasingly recognized as a valid respiratory specimen for SARS-CoV-2 diagnosis. It is easy to collect and suitable for community-wide screening. The optimal timing of collection is currently unknown, and we speculate that an early-morning specimen before oral hygiene and breakfast would increase the diagnostic yield. Methods: Posterior oropharyngeal saliva was collected at 5 different time points within the same day from 18 patients with previously confirmed SARS-CoV-2 infection by molecular testing. Cycle threshold (Ct) values were compared. Results: There was an overall trend of lower Ct values from specimens collected in the early morning, with a gradual decrease of viral load towards nighttime, but reaching statistical significance only when compared with the specimens collected at bedtime. Eight out of 13 subjects had a higher viral load in the early morning than the rest of the 4 time points (before lunch, before teatime at 3 PM, before dinner, before bedtime). Conclusions: The result suggests a diurnal variation of viral shedding from the upper respiratory tract with a trend showing higher viral load in the early morning. For community screening purposes, posterior oropharyngeal saliva could be taken throughout the day, but preferably in the early morning to maximize the yield.
Persistent Identifierhttp://hdl.handle.net/10722/289781
ISSN
2020 Impact Factor: 3.835
2020 SCImago Journal Rankings: 1.546
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorHung, DLL-
dc.contributor.authorLi, X-
dc.contributor.authorChiu, KHY-
dc.contributor.authorYip, CCY-
dc.contributor.authorTo, KKW-
dc.contributor.authorChan, JFW-
dc.contributor.authorSridhar, S-
dc.contributor.authorChung, TWH-
dc.contributor.authorLung, KC-
dc.contributor.authorLiu, RWT-
dc.contributor.authorKwan, GSW-
dc.contributor.authorHung, IFN-
dc.contributor.authorCheng, VCC-
dc.contributor.authorYuen, KY-
dc.date.accessioned2020-10-22T08:17:23Z-
dc.date.available2020-10-22T08:17:23Z-
dc.date.issued2020-
dc.identifier.citationOpen Forum Infectious Diseases, 2020, v. 7 n. 6, article no. ofaa210-
dc.identifier.issn2328-8957-
dc.identifier.urihttp://hdl.handle.net/10722/289781-
dc.description.abstractBackground: Posterior oropharyngeal saliva is increasingly recognized as a valid respiratory specimen for SARS-CoV-2 diagnosis. It is easy to collect and suitable for community-wide screening. The optimal timing of collection is currently unknown, and we speculate that an early-morning specimen before oral hygiene and breakfast would increase the diagnostic yield. Methods: Posterior oropharyngeal saliva was collected at 5 different time points within the same day from 18 patients with previously confirmed SARS-CoV-2 infection by molecular testing. Cycle threshold (Ct) values were compared. Results: There was an overall trend of lower Ct values from specimens collected in the early morning, with a gradual decrease of viral load towards nighttime, but reaching statistical significance only when compared with the specimens collected at bedtime. Eight out of 13 subjects had a higher viral load in the early morning than the rest of the 4 time points (before lunch, before teatime at 3 PM, before dinner, before bedtime). Conclusions: The result suggests a diurnal variation of viral shedding from the upper respiratory tract with a trend showing higher viral load in the early morning. For community screening purposes, posterior oropharyngeal saliva could be taken throughout the day, but preferably in the early morning to maximize the yield.-
dc.languageeng-
dc.publisherOxford University Press (OUP): Policy C. The Journal's web site is located at http://ofid.oxfordjournals.org/-
dc.relation.ispartofOpen Forum Infectious Diseases-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.subjectcoronavirus-
dc.subjectdiagnosis-
dc.subjectSARS-CoV-2-
dc.subjectsaliva-
dc.subjectscreening-
dc.titleEarly-Morning vs Spot Posterior Oropharyngeal Saliva for Diagnosis of SARS-CoV-2 Infection: Implication of Timing of Specimen Collection for Community-Wide Screening-
dc.typeArticle-
dc.identifier.emailHung, DLL: dh2552@hku.hk-
dc.identifier.emailLi, X: xinli@hku.hk-
dc.identifier.emailChiu, KHY: hychiu14@hku.hk-
dc.identifier.emailYip, CCY: yipcyril@hku.hk-
dc.identifier.emailTo, KKW: kelvinto@hku.hk-
dc.identifier.emailChan, JFW: jfwchan@hku.hk-
dc.identifier.emailSridhar, S: sid8998@hku.hk-
dc.identifier.emailHung, IFN: ivanhung@hkucc.hku.hk-
dc.identifier.emailCheng, VCC: vcccheng@hkucc.hku.hk-
dc.identifier.emailYuen, KY: kyyuen@hkucc.hku.hk-
dc.identifier.authorityYip, CCY=rp01721-
dc.identifier.authorityTo, KKW=rp01384-
dc.identifier.authorityChan, JFW=rp01736-
dc.identifier.authoritySridhar, S=rp02249-
dc.identifier.authorityHung, IFN=rp00508-
dc.identifier.authorityYuen, KY=rp00366-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1093/ofid/ofaa210-
dc.identifier.pmid32577428-
dc.identifier.pmcidPMC7299521-
dc.identifier.scopuseid_2-s2.0-85091103273-
dc.identifier.hkuros317161-
dc.identifier.volume7-
dc.identifier.issue6-
dc.identifier.spagearticle no. ofaa210-
dc.identifier.epagearticle no. ofaa210-
dc.identifier.isiWOS:000565181000043-
dc.publisher.placeUnited Kingdom-
dc.identifier.issnl2328-8957-

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