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Article: Encrypted wide-field two-photon microscopy with single-pixel detection and compressed sensing

TitleEncrypted wide-field two-photon microscopy with single-pixel detection and compressed sensing
Authors
KeywordsDifferential detection
Femtosecond fiber lasers
Image collections
Orthogonal basis
Patterned illumination
Issue Date2020
PublisherIOP Publishing, published in association with Japan Society of Applied Physics. The Journal's web site is located at http://iopscience.iop.org/1882-0786/
Citation
Applied Physics Express, 2020, v. 13 n. 3, article no. 032007 How to Cite?
AbstractWe demonstrate a single-pixel two-photon microscopy using a compact femtosecond fiber laser by spatially tailoring the beam into orthogonal basis for patterned illumination. Such wide-field illumination excites a weak two-photon fluorescence signal that can be detected by a photomultiplier tube. An encrypted hybrid basis with random element sequence of Hadamard basis is adopted to illuminate the sample. The hybrid basis shares the same differential detection with Hadamard basis, and greatly reduces the number of measurements compared with random basis. The reduced number of measurements was demonstrated by using compressed sensing, which allows the minimum image collection and transfer bandwidth.
Persistent Identifierhttp://hdl.handle.net/10722/289708
ISSN
2019 Impact Factor: 3.086
2015 SCImago Journal Rankings: 0.766
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorRen, YX-
dc.contributor.authorKong, C-
dc.contributor.authorHe, H-
dc.contributor.authorZeng, X-
dc.contributor.authorTsia, KKT-
dc.contributor.authorWong, KKY-
dc.date.accessioned2020-10-22T08:16:18Z-
dc.date.available2020-10-22T08:16:18Z-
dc.date.issued2020-
dc.identifier.citationApplied Physics Express, 2020, v. 13 n. 3, article no. 032007-
dc.identifier.issn1882-0778-
dc.identifier.urihttp://hdl.handle.net/10722/289708-
dc.description.abstractWe demonstrate a single-pixel two-photon microscopy using a compact femtosecond fiber laser by spatially tailoring the beam into orthogonal basis for patterned illumination. Such wide-field illumination excites a weak two-photon fluorescence signal that can be detected by a photomultiplier tube. An encrypted hybrid basis with random element sequence of Hadamard basis is adopted to illuminate the sample. The hybrid basis shares the same differential detection with Hadamard basis, and greatly reduces the number of measurements compared with random basis. The reduced number of measurements was demonstrated by using compressed sensing, which allows the minimum image collection and transfer bandwidth.-
dc.languageeng-
dc.publisherIOP Publishing, published in association with Japan Society of Applied Physics. The Journal's web site is located at http://iopscience.iop.org/1882-0786/-
dc.relation.ispartofApplied Physics Express-
dc.rightsApplied Physics Express. Copyright © IOP Publishing, published in association with Japan Society of Applied Physics.-
dc.rightsThis is an author-created, un-copyedited version of an article published in Applied Physics Express. IOP Publishing Ltd is not responsible for any errors or omissions in this version of the manuscript or any version derived from it. The Version of Record is available online at http://dx.doi.org/10.35848/1882-0786/ab7409-
dc.subjectDifferential detection-
dc.subjectFemtosecond fiber lasers-
dc.subjectImage collections-
dc.subjectOrthogonal basis-
dc.subjectPatterned illumination-
dc.titleEncrypted wide-field two-photon microscopy with single-pixel detection and compressed sensing-
dc.typeArticle-
dc.identifier.emailRen, YX: yxren@hku.hk-
dc.identifier.emailTsia, KKT: tsia@hku.hk-
dc.identifier.emailWong, KKY: kywong@eee.hku.hk-
dc.identifier.authorityTsia, KKT=rp01389-
dc.identifier.authorityWong, KKY=rp00189-
dc.description.naturepostprint-
dc.identifier.doi10.35848/1882-0786/ab7409-
dc.identifier.scopuseid_2-s2.0-85082708626-
dc.identifier.hkuros316961-
dc.identifier.volume13-
dc.identifier.issue3-
dc.identifier.spagearticle no. 032007-
dc.identifier.epagearticle no. 032007-
dc.identifier.isiWOS:000524725900007-
dc.publisher.placeUnited Kingdom-
dc.identifier.issnl1882-0778-

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