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Article: Surface optimization of gold nanoparticle mass tags for the sensitive detection of protein biomarkers via immuno-capture LI-MS

TitleSurface optimization of gold nanoparticle mass tags for the sensitive detection of protein biomarkers via immuno-capture LI-MS
Authors
TAM, TSCCHENG, YHLok, CNAu Yeung, HYNi, WXWei, XLNg, KM
KeywordsAntigen detections
Cervical cancers
Early diagnosis
HeLa cell lysate
Marker proteins
Issue Date2020
PublisherRoyal Society of Chemistry. The Journal's web site is located at http://www.rsc.org/analyst
Citation
The Analyst, 2020, v. 145 n. 19, p. 6237-6242 How to Cite?
DOI: http://dx.doi.org/10.1039/D0AN01121E
AbstractHPV-induced cervical cancer is one of the most lethal cancers. Therefore, the development of a reliable and accurate method for the early diagnosis of HPV infections is highly important. Here, gold nanoparticles (AuNPs) were utilized as mass tags in an immuno-capture LI-MS assay for the detection of HPV marker proteins. Through the optimization of the amount of antibodies and surface charges on AuNPs, high antigen detection efficiency with minimal non-specific binding was achieved. With optimized antibody-conjugated AuNPs, low attomole amount of HPV proteins in HeLa cell lysate was quantified.
Persistent Identifierhttp://hdl.handle.net/10722/289650
ISSN
0003-2654
2021 Impact Factor: 5.227
2020 SCImago Journal Rankings: 0.998
ISI Accession Number ID
WOS:000573175200024
Grants
Gold@Metal Sulfide (Noble Metal@Semiconductor) Nanoparticles: Enhancement of Laser Desorption/Ionization Efficiency via Plasmon-Exciton Coupling and Its Analytical Application in Mass Spectrometry

 

DC FieldValueLanguage
dc.contributor.authorTAM, TSC-
dc.contributor.authorCHENG, YH-
dc.contributor.authorLok, CN-
dc.contributor.authorAu Yeung, HY-
dc.contributor.authorNi, WX-
dc.contributor.authorWei, XL-
dc.contributor.authorNg, KM-
dc.date.accessioned2020-10-22T08:15:32Z-
dc.date.available2020-10-22T08:15:32Z-
dc.date.issued2020-
dc.identifier.citationThe Analyst, 2020, v. 145 n. 19, p. 6237-6242-
dc.identifier.issn0003-2654-
dc.identifier.urihttp://hdl.handle.net/10722/289650-
dc.description.abstractHPV-induced cervical cancer is one of the most lethal cancers. Therefore, the development of a reliable and accurate method for the early diagnosis of HPV infections is highly important. Here, gold nanoparticles (AuNPs) were utilized as mass tags in an immuno-capture LI-MS assay for the detection of HPV marker proteins. Through the optimization of the amount of antibodies and surface charges on AuNPs, high antigen detection efficiency with minimal non-specific binding was achieved. With optimized antibody-conjugated AuNPs, low attomole amount of HPV proteins in HeLa cell lysate was quantified.-
dc.languageeng-
dc.publisherRoyal Society of Chemistry. The Journal's web site is located at http://www.rsc.org/analyst-
dc.relation.ispartofThe Analyst-
dc.subjectAntigen detections-
dc.subjectCervical cancers-
dc.subjectEarly diagnosis-
dc.subjectHeLa cell lysate-
dc.subjectMarker proteins-
dc.titleSurface optimization of gold nanoparticle mass tags for the sensitive detection of protein biomarkers via immuno-capture LI-MS-
dc.typeArticle-
dc.identifier.emailLok, CN: cnlok@hkucc.hku.hk-
dc.identifier.emailAu Yeung, HY: hoyuay@hku.hk-
dc.identifier.emailNg, KM: kwanmng@hku.hk-
dc.identifier.authorityLok, CN=rp00752-
dc.identifier.authorityAu Yeung, HY=rp01819-
dc.identifier.authorityNg, KM=rp00766-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1039/D0AN01121E-
dc.identifier.pmid32839801-
dc.identifier.scopuseid_2-s2.0-85091808523-
dc.identifier.hkuros315955-
dc.identifier.volume145-
dc.identifier.issue19-
dc.identifier.spage6237-
dc.identifier.epage6242-
dc.identifier.isiWOS:000573175200024-
dc.publisher.placeUnited Kingdom-
dc.relation.projectGold@Metal Sulfide (Noble Metal@Semiconductor) Nanoparticles: Enhancement of Laser Desorption/Ionization Efficiency via Plasmon-Exciton Coupling and Its Analytical Application in Mass Spectrometry-
dc.identifier.issnl0003-2654-

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