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Article: 1-phenyl 2-thiourea (PTU) activates autophagy in zebrafish embryos

Title1-phenyl 2-thiourea (PTU) activates autophagy in zebrafish embryos
Authors
Keywords1-phenyl 2-thiourea
autophagy
melanogenesis
tyrosinase
zebrafish embryo
Issue Date2020
PublisherTaylor & Francis: STM, Behavioural Science and Public Health Titles. The Journal's web site is located at http://www.tandfonline.com/loi/kaup20#.VfwhQUaFOnI
Citation
Autophagy, 2020, Epub 2020-04-22 How to Cite?
Abstract1-phenyl 2-thiourea (PTU) is a Tyr (tyrosinase) inhibitor that is extensively used to block pigmentation and improve optical transparency in zebrafish (Danio rerio) embryo. Here, we reported a previously undescribed effect of PTU on macroautophagy/autophagy in zebrafish embryos. Upon 0.003% PTU treatment, aberrant autophagosome and autolysosome formation, accumulation of lysosomes, and elevated autophagic flux were observed in various tissues and organs of zebrafish embryos, such as skin, brain, and muscle. Similar to PTU treatment, autophagic activation and lysosomal accumulation were also observed in the somatic tyr mutant zebrafish embryos, which suggest that Tyr inhibition may contribute to PTU-induced autophagic activation. Furthermore, we demonstrated that autophagy contributes to pigmentation inhibition, but is not essential to the PTU-induced pigmentation inhibition. With the involvement of autophagy in a wide range of physiological and pathological processes and the routine use of PTU in zebrafish research of autophagy-related processes, these observations raise a novel concern in autophagy-related studies using PTU-treated zebrafish embryos.
Descriptionlink_to_subscribed_fulltext
Persistent Identifierhttp://hdl.handle.net/10722/289594
ISSN
2023 Impact Factor: 14.6
2023 SCImago Journal Rankings: 4.035
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorChen, XK-
dc.contributor.authorKwan, JSK-
dc.contributor.authorChang, RCC-
dc.contributor.authorMa, ACH-
dc.date.accessioned2020-10-22T08:14:49Z-
dc.date.available2020-10-22T08:14:49Z-
dc.date.issued2020-
dc.identifier.citationAutophagy, 2020, Epub 2020-04-22-
dc.identifier.issn1554-8627-
dc.identifier.urihttp://hdl.handle.net/10722/289594-
dc.descriptionlink_to_subscribed_fulltext-
dc.description.abstract1-phenyl 2-thiourea (PTU) is a Tyr (tyrosinase) inhibitor that is extensively used to block pigmentation and improve optical transparency in zebrafish (Danio rerio) embryo. Here, we reported a previously undescribed effect of PTU on macroautophagy/autophagy in zebrafish embryos. Upon 0.003% PTU treatment, aberrant autophagosome and autolysosome formation, accumulation of lysosomes, and elevated autophagic flux were observed in various tissues and organs of zebrafish embryos, such as skin, brain, and muscle. Similar to PTU treatment, autophagic activation and lysosomal accumulation were also observed in the somatic tyr mutant zebrafish embryos, which suggest that Tyr inhibition may contribute to PTU-induced autophagic activation. Furthermore, we demonstrated that autophagy contributes to pigmentation inhibition, but is not essential to the PTU-induced pigmentation inhibition. With the involvement of autophagy in a wide range of physiological and pathological processes and the routine use of PTU in zebrafish research of autophagy-related processes, these observations raise a novel concern in autophagy-related studies using PTU-treated zebrafish embryos.-
dc.languageeng-
dc.publisherTaylor & Francis: STM, Behavioural Science and Public Health Titles. The Journal's web site is located at http://www.tandfonline.com/loi/kaup20#.VfwhQUaFOnI-
dc.relation.ispartofAutophagy-
dc.rightsThis is an electronic version of an article published in [include the complete citation information for the final version of the article as published in the print edition of the journal]. [JOURNAL TITLE] is available online at: http://www.informaworld.com/smpp/ with the open URL of your article.-
dc.subject1-phenyl 2-thiourea-
dc.subjectautophagy-
dc.subjectmelanogenesis-
dc.subjecttyrosinase-
dc.subjectzebrafish embryo-
dc.title1-phenyl 2-thiourea (PTU) activates autophagy in zebrafish embryos-
dc.typeArticle-
dc.identifier.emailChen, XK: kxkchen@HKUCC-COM.hku.hk-
dc.identifier.emailChang, RCC: rccchang@hku.hk-
dc.identifier.authorityChang, RCC=rp00470-
dc.identifier.doi10.1080/15548627.2020.1755119-
dc.identifier.pmid32286915-
dc.identifier.scopuseid_2-s2.0-85083699558-
dc.identifier.hkuros317234-
dc.identifier.volumeEpub 2020-04-22-
dc.identifier.spage1-
dc.identifier.epage10-
dc.identifier.isiWOS:000528180200001-
dc.publisher.placeUnited States-
dc.identifier.issnl1554-8627-

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