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- Publisher Website: 10.1093/cid/ciaa995
- Scopus: eid_2-s2.0-85112125364
- PMID: 32667973
- WOS: WOS:000697378800036
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Article: Severe Acute Respiratory Syndrome Coronavirus 2 Infects and Damages the Mature and Immature Olfactory Sensory Neurons of Hamsters
Title | Severe Acute Respiratory Syndrome Coronavirus 2 Infects and Damages the Mature and Immature Olfactory Sensory Neurons of Hamsters |
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Authors | |
Keywords | SARS-CoV-2 COVID-19 olfactory neuron hamster |
Issue Date | 2020 |
Publisher | Oxford University Press. The Journal's web site is located at http://www.oxfordjournals.org/our_journals/cid/ |
Citation | Clinical Infectious Diseases, 2020, Epub 2020-07-15, p. article no. ciaa995 How to Cite? |
Abstract | Background:
Coronavirus disease 2019 (COVID-19) is primarily an acute respiratory tract infection. Distinctively, a substantial proportion of COVID-19 patients develop olfactory dysfunction. Especially in young patients, loss of smell can be the first or only symptom. The roles of inflammatory obstruction of the olfactory clefts, inflammatory cytokines affecting olfactory neuronal function, destruction of olfactory neurons or their supporting cells, and direct invasion of olfactory bulbs in causing olfactory dysfunction are uncertain.
Methods:
We investigated the location for the pathogenesis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) from the olfactory epithelium (OE) to the olfactory bulb in golden Syrian hamsters.
Results:
After intranasal inoculation with SARS-CoV-2, inflammatory cell infiltration and proinflammatory cytokine/chemokine responses were detected in the nasal turbinate tissues. The responses peaked between 2 and 4 days postinfection, with the highest viral load detected at day 2 postinfection. In addition to the pseudo-columnar ciliated respiratory epithelial cells, SARS-CoV-2 viral antigens were also detected in the mature olfactory sensory neurons labeled by olfactory marker protein, in the less mature olfactory neurons labeled by neuron-specific class III β-tubulin at the more basal position, and in the sustentacular cells, resulting in apoptosis and severe destruction of the OE. During the entire course of infection, SARS-CoV-2 viral antigens were not detected in the olfactory bulb.
Conclusions:
In addition to acute inflammation at the OE, infection of mature and immature olfactory neurons and the supporting sustentacular cells by SARS-CoV-2 may contribute to the unique olfactory dysfunction related to COVID-19, which is not reported with SARS-CoV-2. |
Persistent Identifier | http://hdl.handle.net/10722/289588 |
ISSN | 2023 Impact Factor: 8.2 2023 SCImago Journal Rankings: 3.308 |
PubMed Central ID | |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Zhang, AJ | - |
dc.contributor.author | Lee, ACY | - |
dc.contributor.author | Chu, H | - |
dc.contributor.author | Chan, JFW | - |
dc.contributor.author | Fan, Z | - |
dc.contributor.author | Li, C | - |
dc.contributor.author | Liu, F | - |
dc.contributor.author | Chen, Y | - |
dc.contributor.author | Yuan, S | - |
dc.contributor.author | Poon, VKM | - |
dc.contributor.author | Chan, CCS | - |
dc.contributor.author | Cai, JP | - |
dc.contributor.author | Wu, KLK | - |
dc.contributor.author | Sridhar, S | - |
dc.contributor.author | Chan, YS | - |
dc.contributor.author | Yuen, KY | - |
dc.date.accessioned | 2020-10-22T08:14:44Z | - |
dc.date.available | 2020-10-22T08:14:44Z | - |
dc.date.issued | 2020 | - |
dc.identifier.citation | Clinical Infectious Diseases, 2020, Epub 2020-07-15, p. article no. ciaa995 | - |
dc.identifier.issn | 1058-4838 | - |
dc.identifier.uri | http://hdl.handle.net/10722/289588 | - |
dc.description.abstract | Background: Coronavirus disease 2019 (COVID-19) is primarily an acute respiratory tract infection. Distinctively, a substantial proportion of COVID-19 patients develop olfactory dysfunction. Especially in young patients, loss of smell can be the first or only symptom. The roles of inflammatory obstruction of the olfactory clefts, inflammatory cytokines affecting olfactory neuronal function, destruction of olfactory neurons or their supporting cells, and direct invasion of olfactory bulbs in causing olfactory dysfunction are uncertain. Methods: We investigated the location for the pathogenesis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) from the olfactory epithelium (OE) to the olfactory bulb in golden Syrian hamsters. Results: After intranasal inoculation with SARS-CoV-2, inflammatory cell infiltration and proinflammatory cytokine/chemokine responses were detected in the nasal turbinate tissues. The responses peaked between 2 and 4 days postinfection, with the highest viral load detected at day 2 postinfection. In addition to the pseudo-columnar ciliated respiratory epithelial cells, SARS-CoV-2 viral antigens were also detected in the mature olfactory sensory neurons labeled by olfactory marker protein, in the less mature olfactory neurons labeled by neuron-specific class III β-tubulin at the more basal position, and in the sustentacular cells, resulting in apoptosis and severe destruction of the OE. During the entire course of infection, SARS-CoV-2 viral antigens were not detected in the olfactory bulb. Conclusions: In addition to acute inflammation at the OE, infection of mature and immature olfactory neurons and the supporting sustentacular cells by SARS-CoV-2 may contribute to the unique olfactory dysfunction related to COVID-19, which is not reported with SARS-CoV-2. | - |
dc.language | eng | - |
dc.publisher | Oxford University Press. The Journal's web site is located at http://www.oxfordjournals.org/our_journals/cid/ | - |
dc.relation.ispartof | Clinical Infectious Diseases | - |
dc.rights | Pre-print: Journal Title] ©: [year] [owner as specified on the article] Published by Oxford University Press [on behalf of xxxxxx]. All rights reserved. Pre-print (Once an article is published, preprint notice should be amended to): This is an electronic version of an article published in [include the complete citation information for the final version of the Article as published in the print edition of the Journal.] Post-print: This is a pre-copy-editing, author-produced PDF of an article accepted for publication in [insert journal title] following peer review. The definitive publisher-authenticated version [insert complete citation information here] is available online at: xxxxxxx [insert URL that the author will receive upon publication here]. | - |
dc.subject | SARS-CoV-2 | - |
dc.subject | COVID-19 | - |
dc.subject | olfactory neuron | - |
dc.subject | hamster | - |
dc.title | Severe Acute Respiratory Syndrome Coronavirus 2 Infects and Damages the Mature and Immature Olfactory Sensory Neurons of Hamsters | - |
dc.type | Article | - |
dc.identifier.email | Zhang, AJ: zhangajx@hkucc.hku.hk | - |
dc.identifier.email | Lee, ACY: cyalee@hku.hk | - |
dc.identifier.email | Chu, H: hinchu@hku.hk | - |
dc.identifier.email | Chan, JFW: jfwchan@hku.hk | - |
dc.identifier.email | Fan, Z: fanzm@HKUCC-COM.hku.hk | - |
dc.identifier.email | Li, C: canlee@hku.hk | - |
dc.identifier.email | Liu, F: liuts89@hku.hk | - |
dc.identifier.email | Yuan, S: yuansf@hku.hk | - |
dc.identifier.email | Poon, VKM: vinpoon@hku.hk | - |
dc.identifier.email | Chan, CCS: cschan@hku.hk | - |
dc.identifier.email | Cai, JP: caijuice@hku.hk | - |
dc.identifier.email | Wu, KLK: lwu03@hku.hk | - |
dc.identifier.email | Sridhar, S: sid8998@hku.hk | - |
dc.identifier.email | Chan, YS: yschan@hku.hk | - |
dc.identifier.email | Yuen, KY: kyyuen@hkucc.hku.hk | - |
dc.identifier.authority | Zhang, AJ=rp00413 | - |
dc.identifier.authority | Chu, H=rp02125 | - |
dc.identifier.authority | Chan, JFW=rp01736 | - |
dc.identifier.authority | Yuan, S=rp02640 | - |
dc.identifier.authority | Sridhar, S=rp02249 | - |
dc.identifier.authority | Chan, YS=rp00318 | - |
dc.identifier.authority | Yuen, KY=rp00366 | - |
dc.description.nature | link_to_OA_fulltext | - |
dc.identifier.doi | 10.1093/cid/ciaa995 | - |
dc.identifier.pmid | 32667973 | - |
dc.identifier.pmcid | PMC7454453 | - |
dc.identifier.scopus | eid_2-s2.0-85112125364 | - |
dc.identifier.hkuros | 316904 | - |
dc.identifier.volume | Epub 2020-07-15 | - |
dc.identifier.spage | article no. ciaa995 | - |
dc.identifier.epage | article no. ciaa995 | - |
dc.identifier.isi | WOS:000697378800036 | - |
dc.publisher.place | United States | - |
dc.identifier.issnl | 1058-4838 | - |