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- Publisher Website: 10.1152/ajpcell.00154.2014
- Scopus: eid_2-s2.0-84907569347
- PMID: 24944200
- WOS: WOS:000343244300004
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Article: High-throughput chemical screening identifies AG-490 as a stimulator of aquaporin 2 membrane expression and urine concentration
Title | High-throughput chemical screening identifies AG-490 as a stimulator of aquaporin 2 membrane expression and urine concentration |
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Authors | |
Keywords | EGFR and JAK-2 inhibitor Drug screening Cell signaling Water channels Diabetes insipidus Vasopressin |
Issue Date | 2014 |
Citation | American Journal of Physiology: Cell Physiology, 2014, v. 307, n. 7, p. C597-C605 How to Cite? |
Abstract | © 2014 the American Physiological Society. A reduction or loss of plasma membrane aquaporin 2 (AQP2) in kidney principal cells due to defective vasopressin (VP) signaling through the VP receptor causes excessive urine production, i.e., diabetes insipidus. The amount of AQP2 on the plasma membrane is regulated by a balance of exocytosis and endocytosis and is the rate limiting step for water reabsorption in the collecting duct. We describe here a systematic approach using high-throughput screening (HTS) fol- lowed by in vitro and in vivo assays to discover novel compounds that enhance vasopressin-independent AQP2 membrane expres- sion. We performed initial chemical library screening with a high-throughput exocytosis fluorescence assay using LLC-PK1 cells expressing soluble secreted yellow fluorescent protein and AQP2. Thirty-six candidate exocytosis enhancers were identified. These compounds were then rescreened in AQP2-expressing cells to determine their ability to increase AQP2 membrane accumula- tion. Effective drugs were then applied to kidney slices in vitro. Three compounds, AG-490, β-lapachone, and HA14-1 increased AQP2 membrane accumulation in LLC-PK1 cells, and both AG-490 and -lapachone were also effective in MDCK cells and principal cells in rat kidney slices. Finally, one compound, AG-490 (an EGF receptor and JAK-2 kinase inhibitor), decreased urine volume and increased urine osmolality significantly in the first 2-4 h after a single injection into VP-deficient Brattleboro rats. In conclusion, we have developed a systematic procedure for identi- fying new compounds that modulate AQP2 trafficking using initial HTS followed by in vitro assays in cells and kidney slices, and concluding with in vivo testing in an animal model. |
Persistent Identifier | http://hdl.handle.net/10722/288636 |
ISSN | 2023 Impact Factor: 5.0 2023 SCImago Journal Rankings: 1.711 |
PubMed Central ID | |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Nomura, Naohiro | - |
dc.contributor.author | Nunes, Paula | - |
dc.contributor.author | Bouley, Richard | - |
dc.contributor.author | Nair, Anil V. | - |
dc.contributor.author | Shaw, Stanley | - |
dc.contributor.author | Ueda, Erica | - |
dc.contributor.author | Pathomthongtaweechai, Nutthapoom | - |
dc.contributor.author | Jenny Lu, Hua A. | - |
dc.contributor.author | Brown, Dennis | - |
dc.date.accessioned | 2020-10-12T08:05:28Z | - |
dc.date.available | 2020-10-12T08:05:28Z | - |
dc.date.issued | 2014 | - |
dc.identifier.citation | American Journal of Physiology: Cell Physiology, 2014, v. 307, n. 7, p. C597-C605 | - |
dc.identifier.issn | 0363-6143 | - |
dc.identifier.uri | http://hdl.handle.net/10722/288636 | - |
dc.description.abstract | © 2014 the American Physiological Society. A reduction or loss of plasma membrane aquaporin 2 (AQP2) in kidney principal cells due to defective vasopressin (VP) signaling through the VP receptor causes excessive urine production, i.e., diabetes insipidus. The amount of AQP2 on the plasma membrane is regulated by a balance of exocytosis and endocytosis and is the rate limiting step for water reabsorption in the collecting duct. We describe here a systematic approach using high-throughput screening (HTS) fol- lowed by in vitro and in vivo assays to discover novel compounds that enhance vasopressin-independent AQP2 membrane expres- sion. We performed initial chemical library screening with a high-throughput exocytosis fluorescence assay using LLC-PK1 cells expressing soluble secreted yellow fluorescent protein and AQP2. Thirty-six candidate exocytosis enhancers were identified. These compounds were then rescreened in AQP2-expressing cells to determine their ability to increase AQP2 membrane accumula- tion. Effective drugs were then applied to kidney slices in vitro. Three compounds, AG-490, β-lapachone, and HA14-1 increased AQP2 membrane accumulation in LLC-PK1 cells, and both AG-490 and -lapachone were also effective in MDCK cells and principal cells in rat kidney slices. Finally, one compound, AG-490 (an EGF receptor and JAK-2 kinase inhibitor), decreased urine volume and increased urine osmolality significantly in the first 2-4 h after a single injection into VP-deficient Brattleboro rats. In conclusion, we have developed a systematic procedure for identi- fying new compounds that modulate AQP2 trafficking using initial HTS followed by in vitro assays in cells and kidney slices, and concluding with in vivo testing in an animal model. | - |
dc.language | eng | - |
dc.relation.ispartof | American Journal of Physiology: Cell Physiology | - |
dc.subject | EGFR and JAK-2 inhibitor | - |
dc.subject | Drug screening | - |
dc.subject | Cell signaling | - |
dc.subject | Water channels | - |
dc.subject | Diabetes insipidus | - |
dc.subject | Vasopressin | - |
dc.title | High-throughput chemical screening identifies AG-490 as a stimulator of aquaporin 2 membrane expression and urine concentration | - |
dc.type | Article | - |
dc.description.nature | link_to_OA_fulltext | - |
dc.identifier.doi | 10.1152/ajpcell.00154.2014 | - |
dc.identifier.pmid | 24944200 | - |
dc.identifier.pmcid | PMC4187055 | - |
dc.identifier.scopus | eid_2-s2.0-84907569347 | - |
dc.identifier.volume | 307 | - |
dc.identifier.issue | 7 | - |
dc.identifier.spage | C597 | - |
dc.identifier.epage | C605 | - |
dc.identifier.eissn | 1522-1563 | - |
dc.identifier.isi | WOS:000343244300004 | - |
dc.identifier.issnl | 0363-6143 | - |